BRENDA - Enzyme Database show
show all sequences of 1.1.1.127

An enzyme in the pectinolytic pathway of Erwinia chrysanthemi: 2-Keto-3-deoxygluconate oxidoreductase

Condemine, G.; Hugouvieux-Cotte-Pattat, N.; Robert-Baudouy, J.; J. Gen. Microbiol. 130, 2839-2844 (1984)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Ag+
72% inhibition at 0.01 mM
Dickeya chrysanthemi
ATP
30% inhibition for both reduction and oxidation reaction at 1 mM
Dickeya chrysanthemi
p-chloromercuribenzoate
50% inhibition at 0.02 mM
Dickeya chrysanthemi
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.03
-
NADH
-
Dickeya chrysanthemi
0.34
-
(4S)-4,6-dihydroxy-2,5-dioxohexanoate
-
Dickeya chrysanthemi
0.4
-
NAD+
-
Dickeya chrysanthemi
7.7
-
2-dehydro-3-deoxy-D-gluconate
-
Dickeya chrysanthemi
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
20% activation at 1 mM
Dickeya chrysanthemi
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2-dehydro-3-deoxy-D-gluconate + NAD+
Dickeya chrysanthemi
involved in pectinolysis by some phytopathogenic bacteria
(4S)-4,6-dihydroxy-2,5-dioxohexanoate + NADH
-
Dickeya chrysanthemi
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Dickeya chrysanthemi
-
-
-
Purification (Commentary)
Commentary
Organism
to homogeneity, 2step chromatography
Dickeya chrysanthemi
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Dickeya chrysanthemi
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
39
-
-
Dickeya chrysanthemi
Storage Stability
Storage Stability
Organism
-20C, 2 mM DTT, 6 months
Dickeya chrysanthemi
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-dehydro-3-deoxy-D-gluconate + NAD+
involved in pectinolysis by some phytopathogenic bacteria
285820
Dickeya chrysanthemi
(4S)-4,6-dihydroxy-2,5-dioxohexanoate + NADH
-
285820
Dickeya chrysanthemi
r
pyruvate + NAD+
10% of the activity compared to 2-dehydro-3-deoxy-D-gluconate
285820
Dickeya chrysanthemi
? + NADH
-
285820
Dickeya chrysanthemi
?
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
45
-
-
Dickeya chrysanthemi
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
20
-
stable for 15 h
Dickeya chrysanthemi
46
-
half-life: 10 min, NADH increases thermal stability
Dickeya chrysanthemi
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
7.5
reduction reaction
Dickeya chrysanthemi
10
-
oxidation reaction
Dickeya chrysanthemi
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Dickeya chrysanthemi
NADH
-
Dickeya chrysanthemi
NADP+
53% lower activity than with NAD+
Dickeya chrysanthemi
NADPH
62% lower activity than with NADH
Dickeya chrysanthemi
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Dickeya chrysanthemi
NADH
-
Dickeya chrysanthemi
NADP+
53% lower activity than with NAD+
Dickeya chrysanthemi
NADPH
62% lower activity than with NADH
Dickeya chrysanthemi
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ag+
72% inhibition at 0.01 mM
Dickeya chrysanthemi
ATP
30% inhibition for both reduction and oxidation reaction at 1 mM
Dickeya chrysanthemi
p-chloromercuribenzoate
50% inhibition at 0.02 mM
Dickeya chrysanthemi
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.03
-
NADH
-
Dickeya chrysanthemi
0.34
-
(4S)-4,6-dihydroxy-2,5-dioxohexanoate
-
Dickeya chrysanthemi
0.4
-
NAD+
-
Dickeya chrysanthemi
7.7
-
2-dehydro-3-deoxy-D-gluconate
-
Dickeya chrysanthemi
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
20% activation at 1 mM
Dickeya chrysanthemi
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2-dehydro-3-deoxy-D-gluconate + NAD+
Dickeya chrysanthemi
involved in pectinolysis by some phytopathogenic bacteria
(4S)-4,6-dihydroxy-2,5-dioxohexanoate + NADH
-
Dickeya chrysanthemi
r
Purification (Commentary) (protein specific)
Commentary
Organism
to homogeneity, 2step chromatography
Dickeya chrysanthemi
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Dickeya chrysanthemi
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
39
-
-
Dickeya chrysanthemi
Storage Stability (protein specific)
Storage Stability
Organism
-20C, 2 mM DTT, 6 months
Dickeya chrysanthemi
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-dehydro-3-deoxy-D-gluconate + NAD+
involved in pectinolysis by some phytopathogenic bacteria
285820
Dickeya chrysanthemi
(4S)-4,6-dihydroxy-2,5-dioxohexanoate + NADH
-
285820
Dickeya chrysanthemi
r
pyruvate + NAD+
10% of the activity compared to 2-dehydro-3-deoxy-D-gluconate
285820
Dickeya chrysanthemi
? + NADH
-
285820
Dickeya chrysanthemi
?
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
45
-
-
Dickeya chrysanthemi
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
20
-
stable for 15 h
Dickeya chrysanthemi
46
-
half-life: 10 min, NADH increases thermal stability
Dickeya chrysanthemi
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
7.5
reduction reaction
Dickeya chrysanthemi
10
-
oxidation reaction
Dickeya chrysanthemi
Other publictions for EC 1.1.1.127
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
285820
Condemine
-
An enzyme in the pectinolytic ...
Dickeya chrysanthemi
J. Gen. Microbiol.
130
2839-2844
1984
-
-
-
-
-
-
3
4
-
1
-
1
-
1
-
-
1
-
-
1
1
1
2
-
1
-
2
-
2
-
-
4
-
-
-
-
-
-
4
-
-
-
-
3
-
4
-
1
-
1
-
-
-
1
-
1
1
1
2
-
1
-
2
-
2
-
-
-
-
-
-
-
-
-
285821
Preiss
Polygalacturonic acid metaboli ...
Pseudomonas sp.
J. Biol. Chem.
238
1577-1583
1963
-
-
-
-
-
1
-
-
-
-
-
1
-
1
-
-
1
-
-
1
1
1
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
1
-
-
-
1
-
1
1
1
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-