General Stability | Organism |
---|---|
unstable in the absence of NADP+ | Pseudomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
p-hydroxymercuribenzoate | 26% inhibition at 0.1 mM | Pseudomonas sp. | |
Tris-HCl | 67% inhibition at pH 7.5, 50 mM | Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.032 | - |
NADPH | - |
Pseudomonas sp. | |
0.36 | - |
(4S,5S)-4,5-dihydroxy-2,6-dioxohexanoate | - |
Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(4S,5S)-4,5-dihydroxy-2,6-dioxohexanoate + NADPH | Pseudomonas sp. | irreversible at pH 7.0, 1% reversibility at pH 10.0 | 2-dehydro-3-deoxy-D-gluconate + NADP+ | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
alginate-adapted | - |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography techniques | Pseudomonas sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | inducible enzyme synthesis by growth in alginate-containing medium | Pseudomonas sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
15.9 | - |
- |
Pseudomonas sp. |
Storage Stability | Organism |
---|---|
0-3°C, 1 mM NADP+, 90% retaining activity after 3 weeks | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(4S,5S)-4,5-dihydroxy-2,6-dioxohexanoate + NADPH | irreversible at pH 7.0, 1% reversibility at pH 10.0 | Pseudomonas sp. | 2-dehydro-3-deoxy-D-gluconate + NADP+ | - |
ir |
Synonyms | Comment | Organism |
---|---|---|
ketodeoxygluconate dehydrogenase | - |
Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | - |
Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | 7.2% of the rate compared to NADPH | Pseudomonas sp. | |
NADPH | - |
Pseudomonas sp. |