BRENDA - Enzyme Database show
show all sequences of 1.1.1.126

Alginic acid metabolism in bacteria. II. The enzymatic reduction of 4-deoxy-L-erythro-5-hexoseulose uronic acid to 2-keto-3-deoxy-D-gluconic acid

Preiss, J.; Ashwell, G.; J. Biol. Chem. 237, 317-321 (1962)

Data extracted from this reference:

General Stability
General Stability
Organism
unstable in the absence of NADP+
Pseudomonas sp.
Inhibitors
Inhibitors
Commentary
Organism
Structure
p-hydroxymercuribenzoate
26% inhibition at 0.1 mM
Pseudomonas sp.
Tris-HCl
67% inhibition at pH 7.5, 50 mM
Pseudomonas sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.032
-
NADPH
-
Pseudomonas sp.
0.36
-
(4S,5S)-4,5-dihydroxy-2,6-dioxohexanoate
-
Pseudomonas sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(4S,5S)-4,5-dihydroxy-2,6-dioxohexanoate + NADPH
Pseudomonas sp.
irreversible at pH 7.0, 1% reversibility at pH 10.0
2-dehydro-3-deoxy-D-gluconate + NADP+
-
Pseudomonas sp.
ir
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas sp.
-
alginate-adapted
-
Purification (Commentary)
Commentary
Organism
to homogeneity, chromatography techniques
Pseudomonas sp.
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
inducible enzyme synthesis by growth in alginate-containing medium
Pseudomonas sp.
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
15.9
-
-
Pseudomonas sp.
Storage Stability
Storage Stability
Organism
0-3°C, 1 mM NADP+, 90% retaining activity after 3 weeks
Pseudomonas sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(4S,5S)-4,5-dihydroxy-2,6-dioxohexanoate + NADPH
irreversible at pH 7.0, 1% reversibility at pH 10.0
285819
Pseudomonas sp.
2-dehydro-3-deoxy-D-gluconate + NADP+
-
285819
Pseudomonas sp.
ir
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
7.5
-
Pseudomonas sp.
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
7.2% of the rate compared to NADPH
Pseudomonas sp.
NADPH
-
Pseudomonas sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
7.2% of the rate compared to NADPH
Pseudomonas sp.
NADPH
-
Pseudomonas sp.
General Stability (protein specific)
General Stability
Organism
unstable in the absence of NADP+
Pseudomonas sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
p-hydroxymercuribenzoate
26% inhibition at 0.1 mM
Pseudomonas sp.
Tris-HCl
67% inhibition at pH 7.5, 50 mM
Pseudomonas sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.032
-
NADPH
-
Pseudomonas sp.
0.36
-
(4S,5S)-4,5-dihydroxy-2,6-dioxohexanoate
-
Pseudomonas sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(4S,5S)-4,5-dihydroxy-2,6-dioxohexanoate + NADPH
Pseudomonas sp.
irreversible at pH 7.0, 1% reversibility at pH 10.0
2-dehydro-3-deoxy-D-gluconate + NADP+
-
Pseudomonas sp.
ir
Purification (Commentary) (protein specific)
Commentary
Organism
to homogeneity, chromatography techniques
Pseudomonas sp.
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
inducible enzyme synthesis by growth in alginate-containing medium
Pseudomonas sp.
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
15.9
-
-
Pseudomonas sp.
Storage Stability (protein specific)
Storage Stability
Organism
0-3°C, 1 mM NADP+, 90% retaining activity after 3 weeks
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(4S,5S)-4,5-dihydroxy-2,6-dioxohexanoate + NADPH
irreversible at pH 7.0, 1% reversibility at pH 10.0
285819
Pseudomonas sp.
2-dehydro-3-deoxy-D-gluconate + NADP+
-
285819
Pseudomonas sp.
ir
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
7.5
-
Pseudomonas sp.
Other publictions for EC 1.1.1.126
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
285819
Preiss
Alginic acid metabolism in bac ...
Pseudomonas sp.
J. Biol. Chem.
237
317-321
1962
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