BRENDA - Enzyme Database show
show all sequences of 1.1.1.125

Microbial metabolism of 2-deoxyglucose; 2-deoxygluconic acid dehydrogenase

Eichhorn, M.M.; Cynkin, M.A.; Biochemistry 4, 159-165 (1965)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
FMN
stimulatory effect
Pseudomonas sp.
Inhibitors
Inhibitors
Commentary
Organism
Structure
NAD+
inhibition at high concentrations
Pseudomonas sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.54
5
2-deoxy-D-gluconate
-
Pseudomonas sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2-deoxy-D-gluconate + NAD+
Pseudomonas sp.
-
3-dehydro-2-deoxy-D-gluconate + NADH
-
Pseudomonas sp.
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas sp.
-
-
-
Purification (Commentary)
Commentary
Organism
partial
Pseudomonas sp.
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
inducible enzyme synthesis by growth in 2-deoxy-D-gluconate containing medium
Pseudomonas sp.
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
32
-
-
Pseudomonas sp.
Storage Stability
Storage Stability
Organism
-5░C, 10 days
Pseudomonas sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-deoxy-D-gluconate + NAD+
-
285818
Pseudomonas sp.
3-dehydro-2-deoxy-D-gluconate + NADH
-
285818
Pseudomonas sp.
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.9
8.9
-
Pseudomonas sp.
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
can not be replaced by NADP+
Pseudomonas sp.
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
FMN
stimulatory effect
Pseudomonas sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
can not be replaced by NADP+
Pseudomonas sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
NAD+
inhibition at high concentrations
Pseudomonas sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.54
5
2-deoxy-D-gluconate
-
Pseudomonas sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2-deoxy-D-gluconate + NAD+
Pseudomonas sp.
-
3-dehydro-2-deoxy-D-gluconate + NADH
-
Pseudomonas sp.
?
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Pseudomonas sp.
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
inducible enzyme synthesis by growth in 2-deoxy-D-gluconate containing medium
Pseudomonas sp.
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
32
-
-
Pseudomonas sp.
Storage Stability (protein specific)
Storage Stability
Organism
-5░C, 10 days
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-deoxy-D-gluconate + NAD+
-
285818
Pseudomonas sp.
3-dehydro-2-deoxy-D-gluconate + NADH
-
285818
Pseudomonas sp.
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.9
8.9
-
Pseudomonas sp.
Other publictions for EC 1.1.1.125
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
726324
Rothe
Novel insights into E. colis h ...
Escherichia coli
PLoS ONE
8
e56906
2013
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4
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3
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3
-
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-
-
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-
1
1
1
1
-
-
285818
Eichhorn
Microbial metabolism of 2-deox ...
Pseudomonas sp.
Biochemistry
4
159-165
1965
1
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1
1
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1
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1
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1
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1
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1
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1
1
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1
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1
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