BRENDA - Enzyme Database show
show all sequences of 1.1.1.124

5-Keto-D-fructose. IV. A specific reduced nicotinamide adenine dinucleotide phosphate-linked reductase from Gluconobacter cerinus

Avigad, G.; Englard, S.; Pifko, S.; J. Biol. Chem. 241, 373-378 (1966)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
D-fructose
inhibition of 5-keto-D-frucotse reduction, Ki: 82 mM
Gluconobacter cerinus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.002
-
NADPH
-
Gluconobacter cerinus
1.3
-
NADP+
-
Gluconobacter cerinus
4.2
-
5-Keto-D-fructose 1-phosphate
-
Gluconobacter cerinus
4.5
-
5-keto-D-fructose
-
Gluconobacter cerinus
70
-
D-fructose
-
Gluconobacter cerinus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-fructose + NADP+
Gluconobacter cerinus
predominant formation of D-fructose
5-dehydro-D-fructose + NADPH
-
Gluconobacter cerinus
r
D-fructose + NADP+
Gluconobacter cerinus
highly specific for D-fructose
5-dehydro-D-fructose + NADPH
-
Gluconobacter cerinus
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Gluconobacter cerinus
-
-
-
Purification (Commentary)
Commentary
Organism
high purity
Gluconobacter cerinus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Gluconobacter cerinus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
627
-
-
Gluconobacter cerinus
Storage Stability
Storage Stability
Organism
4°C, stable for 3-4 weeks
Gluconobacter cerinus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5-keto-D-fructose 1-phosphate + NADPH + H+
16% of the rate compared to 5-keto-D-fructose
285817
Gluconobacter cerinus
D-fructose 1-phosphate + NADP+
-
285817
Gluconobacter cerinus
?
D-fructose + NADP+
predominant formation of D-fructose
285817
Gluconobacter cerinus
5-dehydro-D-fructose + NADPH
-
285817
Gluconobacter cerinus
r
D-fructose + NADP+
highly specific for D-fructose
285817
Gluconobacter cerinus
5-dehydro-D-fructose + NADPH
-
285817
Gluconobacter cerinus
r
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
NADPH oxidation in phosphate buffer
Gluconobacter cerinus
7.4
-
NADPH oxidation in Tris-HCl buffer
Gluconobacter cerinus
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
cannot be replaced by NADH
Gluconobacter cerinus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
cannot be replaced by NADH
Gluconobacter cerinus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
D-fructose
inhibition of 5-keto-D-frucotse reduction, Ki: 82 mM
Gluconobacter cerinus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.002
-
NADPH
-
Gluconobacter cerinus
1.3
-
NADP+
-
Gluconobacter cerinus
4.2
-
5-Keto-D-fructose 1-phosphate
-
Gluconobacter cerinus
4.5
-
5-keto-D-fructose
-
Gluconobacter cerinus
70
-
D-fructose
-
Gluconobacter cerinus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-fructose + NADP+
Gluconobacter cerinus
predominant formation of D-fructose
5-dehydro-D-fructose + NADPH
-
Gluconobacter cerinus
r
D-fructose + NADP+
Gluconobacter cerinus
highly specific for D-fructose
5-dehydro-D-fructose + NADPH
-
Gluconobacter cerinus
r
Purification (Commentary) (protein specific)
Commentary
Organism
high purity
Gluconobacter cerinus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Gluconobacter cerinus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
627
-
-
Gluconobacter cerinus
Storage Stability (protein specific)
Storage Stability
Organism
4°C, stable for 3-4 weeks
Gluconobacter cerinus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5-keto-D-fructose 1-phosphate + NADPH + H+
16% of the rate compared to 5-keto-D-fructose
285817
Gluconobacter cerinus
D-fructose 1-phosphate + NADP+
-
285817
Gluconobacter cerinus
?
D-fructose + NADP+
predominant formation of D-fructose
285817
Gluconobacter cerinus
5-dehydro-D-fructose + NADPH
-
285817
Gluconobacter cerinus
r
D-fructose + NADP+
highly specific for D-fructose
285817
Gluconobacter cerinus
5-dehydro-D-fructose + NADPH
-
285817
Gluconobacter cerinus
r
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
NADPH oxidation in phosphate buffer
Gluconobacter cerinus
7.4
-
NADPH oxidation in Tris-HCl buffer
Gluconobacter cerinus
Other publictions for EC 1.1.1.124
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
285814
Schrimsher
Purification and characterizat ...
Erwinia sp.
Biochem. J.
253
511-516
1988
-
-
-
-
-
-
3
7
-
-
2
1
-
1
-
-
1
-
-
1
1
1
2
1
-
-
-
-
2
-
-
2
-
-
-
-
-
-
2
-
-
-
-
3
-
7
-
-
2
1
-
-
-
1
-
1
1
1
2
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
285815
Ameyama
-
5-keto-D-Fructose reductase of ...
Gluconobacter oxydans
Agric. Biol. Chem.
45
863-869
1981
-
-
-
1
-
-
1
2
-
-
2
1
-
1
-
-
1
-
-
1
2
-
1
1
-
-
-
-
2
-
1
1
-
-
-
-
-
-
1
1
-
-
-
1
-
2
-
-
2
1
-
-
-
1
-
1
2
-
1
1
-
-
-
-
2
-
1
-
-
-
-
-
-
-
285816
Englard
-
5-keto-D-Fructose reductase fr ...
Gluconobacter cerinus
Methods Enzymol.
41B
127-131
1978
-
-
-
-
-
-
1
5
-
-
-
2
-
1
-
-
1
-
-
1
1
1
3
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
5
-
-
-
2
-
-
-
1
-
1
1
1
3
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
285817
Avigad
5-Keto-D-fructose. IV. A speci ...
Gluconobacter cerinus
J. Biol. Chem.
241
373-378
1966
-
-
-
-
-
-
1
5
-
-
-
2
-
1
-
-
1
-
-
1
1
1
3
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
5
-
-
-
2
-
-
-
1
-
1
1
1
3
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-