BRENDA - Enzyme Database show
show all sequences of 1.1.1.124

5-keto-D-Fructose reductase of Gluconobacter industrius: Purification, crystallization and properties

Ameyama, M.; Matsushita, K.; Shinagawa, E.; Adachi, O.; Agric. Biol. Chem. 45, 863-869 (1981)
No PubMed abstract available

Data extracted from this reference:

Crystallization (Commentary)
Crystallization
Organism
ammonium sulfate precipitation
Gluconobacter oxydans
Inhibitors
Inhibitors
Commentary
Organism
Structure
sulfhydryl reagents
-
Gluconobacter oxydans
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.008
-
NADPH
-
Gluconobacter oxydans
4.5
-
5-keto-D-fructose
-
Gluconobacter oxydans
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
25000
-
4 * 25000, SDS-PAGE
Gluconobacter oxydans
100000
-
gel filtration
Gluconobacter oxydans
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-fructose + NADP+
Gluconobacter oxydans
predominant reduction of 5-keto-D-fructose
5-dehydro-D-fructose + NADPH
-
Gluconobacter oxydans
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Gluconobacter oxydans
-
-
-
Purification (Commentary)
Commentary
Organism
high purity to crystalline form, chromatography techniques
Gluconobacter oxydans
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Gluconobacter oxydans
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3
5
D-fructose oxidation
Gluconobacter oxydans
620
-
5-keto-D-fructose reduction
Gluconobacter oxydans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-fructose + NADP+
predominant reduction of 5-keto-D-fructose
285815
Gluconobacter oxydans
5-dehydro-D-fructose + NADPH
-
285815
Gluconobacter oxydans
r
Subunits
Subunits
Commentary
Organism
tetramer
4 * 25000, SDS-PAGE
Gluconobacter oxydans
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
5-keto-D-fructose reduction
Gluconobacter oxydans
8.5
10
D-fructose oxidation
Gluconobacter oxydans
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
4
8
stable between
Gluconobacter oxydans
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
highly specific
Gluconobacter oxydans
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
highly specific
Gluconobacter oxydans
Crystallization (Commentary) (protein specific)
Crystallization
Organism
ammonium sulfate precipitation
Gluconobacter oxydans
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
sulfhydryl reagents
-
Gluconobacter oxydans
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.008
-
NADPH
-
Gluconobacter oxydans
4.5
-
5-keto-D-fructose
-
Gluconobacter oxydans
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
25000
-
4 * 25000, SDS-PAGE
Gluconobacter oxydans
100000
-
gel filtration
Gluconobacter oxydans
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-fructose + NADP+
Gluconobacter oxydans
predominant reduction of 5-keto-D-fructose
5-dehydro-D-fructose + NADPH
-
Gluconobacter oxydans
r
Purification (Commentary) (protein specific)
Commentary
Organism
high purity to crystalline form, chromatography techniques
Gluconobacter oxydans
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Gluconobacter oxydans
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3
5
D-fructose oxidation
Gluconobacter oxydans
620
-
5-keto-D-fructose reduction
Gluconobacter oxydans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-fructose + NADP+
predominant reduction of 5-keto-D-fructose
285815
Gluconobacter oxydans
5-dehydro-D-fructose + NADPH
-
285815
Gluconobacter oxydans
r
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 25000, SDS-PAGE
Gluconobacter oxydans
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
5-keto-D-fructose reduction
Gluconobacter oxydans
8.5
10
D-fructose oxidation
Gluconobacter oxydans
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
4
8
stable between
Gluconobacter oxydans
Other publictions for EC 1.1.1.124
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
285814
Schrimsher
Purification and characterizat ...
Erwinia sp.
Biochem. J.
253
511-516
1988
-
-
-
-
-
-
3
7
-
-
2
1
-
1
-
-
1
-
-
1
1
1
2
1
-
-
-
-
2
-
-
2
-
-
-
-
-
-
2
-
-
-
-
3
-
7
-
-
2
1
-
-
-
1
-
1
1
1
2
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
285815
Ameyama
-
5-keto-D-Fructose reductase of ...
Gluconobacter oxydans
Agric. Biol. Chem.
45
863-869
1981
-
-
-
1
-
-
1
2
-
-
2
1
-
1
-
-
1
-
-
1
2
-
1
1
-
-
-
-
2
-
1
1
-
-
-
-
-
-
1
1
-
-
-
1
-
2
-
-
2
1
-
-
-
1
-
1
2
-
1
1
-
-
-
-
2
-
1
-
-
-
-
-
-
-
285816
Englard
-
5-keto-D-Fructose reductase fr ...
Gluconobacter cerinus
Methods Enzymol.
41B
127-131
1978
-
-
-
-
-
-
1
5
-
-
-
2
-
1
-
-
1
-
-
1
1
1
3
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
5
-
-
-
2
-
-
-
1
-
1
1
1
3
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
285817
Avigad
5-Keto-D-fructose. IV. A speci ...
Gluconobacter cerinus
J. Biol. Chem.
241
373-378
1966
-
-
-
-
-
-
1
5
-
-
-
2
-
1
-
-
1
-
-
1
1
1
3
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
5
-
-
-
2
-
-
-
1
-
1
1
1
3
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-