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show all sequences of 1.1.1.124

Purification and characterization of 5-ketofructose reductase from Erwinia citreus

Schrimsher, J.L.; Wingfield, P.T.; Bernard, A.; Mattaliano, R.; Payton, M.A.; Biochem. J. 253, 511-516 (1988)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
D-fructose
non-competitive inhibition
Erwinia sp.
NADP+
competitive inhibition of D-fructose synthesis, non-competitive inhibition of 5-keto-fructose synthesis
Erwinia sp.
NADPH
competitive inhibition of D-fructose oxidation
Erwinia sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.053
-
NADP+
-
Erwinia sp.
0.071
-
NADPH
-
Erwinia sp.
0.32
-
NADH
-
Erwinia sp.
1.97
-
NAD+
-
Erwinia sp.
9.1
-
D-fructose
-
Erwinia sp.
12.7
-
5-keto-D-fructose
-
Erwinia sp.
140
-
D-arabinose
-
Erwinia sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
1 * 40000, SDS-PAGE
Erwinia sp.
50000
-
gel filtration, sedimentation equilibrium measurement
Erwinia sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-fructose + NADP+
Erwinia sp.
highly specific for D-fructose
5-dehydro-D-fructose + NADPH
-
Erwinia sp.
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Erwinia sp.
-
-
-
Purification (Commentary)
Commentary
Organism
to homogeneity, chromatography techniques
Erwinia sp.
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Erwinia sp.
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2450
-
-
Erwinia sp.
Storage Stability
Storage Stability
Organism
-20°C, 30 mM Tris-HCl, pH 7.8, 3 months
Erwinia sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-arabinose + NADP+
5fold lower activity than for D-fructose
285814
Erwinia sp.
? + NADPH
-
285814
Erwinia sp.
?
D-fructose + NADP+
highly specific for D-fructose
285814
Erwinia sp.
5-dehydro-D-fructose + NADPH
-
285814
Erwinia sp.
r
Subunits
Subunits
Commentary
Organism
monomer
1 * 40000, SDS-PAGE
Erwinia sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
reduction of 5-keto-D-fructose
Erwinia sp.
9.5
-
oxidation of D-fructose
Erwinia sp.
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
substitutes for NADPH
Erwinia sp.
NADPH
oxidized and reduced cofactors bind to the same site of the enzyme
Erwinia sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
substitutes for NADPH
Erwinia sp.
NADPH
oxidized and reduced cofactors bind to the same site of the enzyme
Erwinia sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
D-fructose
non-competitive inhibition
Erwinia sp.
NADP+
competitive inhibition of D-fructose synthesis, non-competitive inhibition of 5-keto-fructose synthesis
Erwinia sp.
NADPH
competitive inhibition of D-fructose oxidation
Erwinia sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.053
-
NADP+
-
Erwinia sp.
0.071
-
NADPH
-
Erwinia sp.
0.32
-
NADH
-
Erwinia sp.
1.97
-
NAD+
-
Erwinia sp.
9.1
-
D-fructose
-
Erwinia sp.
12.7
-
5-keto-D-fructose
-
Erwinia sp.
140
-
D-arabinose
-
Erwinia sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
1 * 40000, SDS-PAGE
Erwinia sp.
50000
-
gel filtration, sedimentation equilibrium measurement
Erwinia sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-fructose + NADP+
Erwinia sp.
highly specific for D-fructose
5-dehydro-D-fructose + NADPH
-
Erwinia sp.
r
Purification (Commentary) (protein specific)
Commentary
Organism
to homogeneity, chromatography techniques
Erwinia sp.
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Erwinia sp.
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2450
-
-
Erwinia sp.
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, 30 mM Tris-HCl, pH 7.8, 3 months
Erwinia sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-arabinose + NADP+
5fold lower activity than for D-fructose
285814
Erwinia sp.
? + NADPH
-
285814
Erwinia sp.
?
D-fructose + NADP+
highly specific for D-fructose
285814
Erwinia sp.
5-dehydro-D-fructose + NADPH
-
285814
Erwinia sp.
r
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 40000, SDS-PAGE
Erwinia sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
reduction of 5-keto-D-fructose
Erwinia sp.
9.5
-
oxidation of D-fructose
Erwinia sp.
Other publictions for EC 1.1.1.124
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
285814
Schrimsher
Purification and characterizat ...
Erwinia sp.
Biochem. J.
253
511-516
1988
-
-
-
-
-
-
3
7
-
-
2
1
-
1
-
-
1
-
-
1
1
1
2
1
-
-
-
-
2
-
-
2
-
-
-
-
-
-
2
-
-
-
-
3
-
7
-
-
2
1
-
-
-
1
-
1
1
1
2
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
285815
Ameyama
-
5-keto-D-Fructose reductase of ...
Gluconobacter oxydans
Agric. Biol. Chem.
45
863-869
1981
-
-
-
1
-
-
1
2
-
-
2
1
-
1
-
-
1
-
-
1
2
-
1
1
-
-
-
-
2
-
1
1
-
-
-
-
-
-
1
1
-
-
-
1
-
2
-
-
2
1
-
-
-
1
-
1
2
-
1
1
-
-
-
-
2
-
1
-
-
-
-
-
-
-
285816
Englard
-
5-keto-D-Fructose reductase fr ...
Gluconobacter cerinus
Methods Enzymol.
41B
127-131
1978
-
-
-
-
-
-
1
5
-
-
-
2
-
1
-
-
1
-
-
1
1
1
3
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
5
-
-
-
2
-
-
-
1
-
1
1
1
3
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
285817
Avigad
5-Keto-D-fructose. IV. A speci ...
Gluconobacter cerinus
J. Biol. Chem.
241
373-378
1966
-
-
-
-
-
-
1
5
-
-
-
2
-
1
-
-
1
-
-
1
1
1
3
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
5
-
-
-
2
-
-
-
1
-
1
1
1
3
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-