BRENDA - Enzyme Database show
show all sequences of 1.1.1.123

Crystal structure of the NADP(H)-dependent ketose reductase from Bemisia argentifolii at 2.3 A resolution

Banfield, M.J.; Salvucci, M.E.; Baker, E.N.; Smith, C.A.; J. Mol. Biol. 306, 239-250 (2001)

Data extracted from this reference:

Metals/Ions
Metals/Ions
Commentary
Organism
Structure
phosphate
in active site, likely position for the adenyl phasphate of NADP(H)
Bemisia argentifolii
Zn2+
catalytical, conserved sites of the sorbitol dehydrogenase sub-family
Bemisia argentifolii
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
additional information
-
monomer has a very similar fold to the alcohol dehydrogenases
Bemisia argentifolii
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bemisia argentifolii
-
adult silverleaf whitefly
-
Purification (Commentary)
Commentary
Organism
-
Bemisia argentifolii
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
fructose + NADPH
-
285812
Bemisia argentifolii
sorbitol + NADP+
-
285812
Bemisia argentifolii
-
Subunits
Subunits
Commentary
Organism
tetramer
-
Bemisia argentifolii
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Bemisia argentifolii
NADPH
-
Bemisia argentifolii
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Bemisia argentifolii
NADPH
-
Bemisia argentifolii
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
phosphate
in active site, likely position for the adenyl phasphate of NADP(H)
Bemisia argentifolii
Zn2+
catalytical, conserved sites of the sorbitol dehydrogenase sub-family
Bemisia argentifolii
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
additional information
-
monomer has a very similar fold to the alcohol dehydrogenases
Bemisia argentifolii
Purification (Commentary) (protein specific)
Commentary
Organism
-
Bemisia argentifolii
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
fructose + NADPH
-
285812
Bemisia argentifolii
sorbitol + NADP+
-
285812
Bemisia argentifolii
-
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
-
Bemisia argentifolii
Other publictions for EC 1.1.1.123
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
285812
Banfield
Crystal structure of the NADP( ...
Bemisia argentifolii
J. Mol. Biol.
306
239-250
2001
-
-
-
-
-
-
-
-
-
2
1
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
1
-
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285810
Salvucci
Effect of high temperature on ...
Bemisia argentifolii
J. Insect Physiol.
45
21-27
1999
-
-
-
-
-
-
-
1
-
-
-
1
-
1
-
-
-
-
-
-
2
-
2
-
1
1
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
2
-
2
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
285811
Wolfe
Molecular basis for thermoprot ...
Bemisia argentifolii
Insect Biochem. Mol. Biol.
29
113-120
1999
-
-
1
-
1
-
-
-
-
1
4
-
-
1
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
1
4
-
-
-
-
1
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
285813
Salvucci
-
Purification and properties of ...
Bemisia argentifolii
Insect Biochem. Mol. Biol.
28
357-363
1998
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
2
-
-
1
1
2
-
-
-
2
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
1
1
2
-
-
-
2
-
-
-
-
-
-
-
-
-
285807
Yagi
-
Purification and properties of ...
Corynebacterium sp.
J. Ferment. Bioeng.
67
212-214
1989
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
-
1
-
-
-
1
-
1
1
-
1
-
-
1
1
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
1
-
-
-
-
1
-
-
1
-
1
1
-
1
-
-
1
1
-
-
-
-
-
-
-
-
285808
Englard
5-keto-D-Fructose reductase fr ...
Bacillus megaterium, Bacillus subtilis 168-2, Bacillus subtilis, Brevibacillus brevis, Cyberlindnera jadinii, Enterobacter aerogenes, Enterobacter cloacae, Gluconobacter oxydans, no activity in Candida tropicalis, Proteus vulgaris, Saccharomyces cerevisiae
Methods Enzymol.
41B
132-138
1975
-
-
-
-
-
-
2
3
-
-
-
-
-
12
-
-
1
-
-
-
1
1
2
-
1
-
-
-
2
-
-
2
-
-
-
-
-
-
2
-
-
-
-
2
-
3
-
-
-
-
-
-
-
1
-
-
1
1
2
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
285809
Englard
5-keto-D-Fructose. VI. A speci ...
Bacillus megaterium, Bacillus subtilis 168-2, Bacillus subtilis, Brevibacillus brevis, Cyberlindnera jadinii, Enterobacter aerogenes, Enterobacter cloacae, Gluconobacter cerinus, Gluconobacter oxydans, no activity in Bacillus cereus, no activity in Candida tropicalis, no activity in Escherichia coli K12, Proteus vulgaris, Saccharomyces cerevisiae
J. Biol. Chem.
245
1311-1318
1970
-
-
-
-
-
-
2
3
-
-
-
-
-
16
-
-
1
-
-
-
1
-
2
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
1
-
-
-
-
2
-
3
-
-
-
-
-
-
-
1
-
-
1
-
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-