Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli XL1-Blue | Bemisia argentifolii |
Protein Variants | Comment | Organism |
---|---|---|
additional information | addition of Arg and loss of Asp decrease the electric potential of the adenosine ribose-binding-pocket, creating an environment favorable for NADPH-binding, the replacement of an Asp with an Ala and the adjacent Leu with an Arg change the potential | Bemisia argentifolii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | catalytical and structural zinc-coordinating residues | Bemisia argentifolii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
amino acid sequence has 60% identity with sheep liver, 41% with Bombyx mori sorbitol dehydrogenase and 21% with horse liver alcohol dehydrogenase | Bemisia argentifolii |
38160 | - |
calculation from polypeptide sequence of 352 amino acids | Bemisia argentifolii |
38700 | - |
4 * 38700, SDS-PAGE | Bemisia argentifolii |
39000 | - |
4 * 39000, recombinant enzyme, SDS-PAGE | Bemisia argentifolii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bemisia argentifolii | - |
adult silverleaf whitefly | - |
Purification (Comment) | Organism |
---|---|
- |
Bemisia argentifolii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
fructose + NADPH | - |
Bemisia argentifolii | sorbitol + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 38700, SDS-PAGE | Bemisia argentifolii |
tetramer | 4 * 39000, recombinant enzyme, SDS-PAGE | Bemisia argentifolii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 41 | sorbitol content increases in a temperature dependent manner | Bemisia argentifolii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | almost exclusive preference/requirement for NADPH | Bemisia argentifolii |