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Literature summary for 1.1.1.123 extracted from

  • Englard, S.; Avigad, G.
    5-keto-D-Fructose reductase from yeast (1975), Methods Enzymol., 41B, 132-138.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
NADP+ competitive inhibitor Saccharomyces cerevisiae
p-hydroxymercuribenzoate 0.4 mM and 1 mM inhibit activity to 21% and 41% respectively Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.046
-
NADPH pH 7.5, 30°C Saccharomyces cerevisiae
0.29
-
5-Keto-D-fructose 1-phosphate
-
Saccharomyces cerevisiae
1
-
5-keto-D-fructose pH 7.5, 30°C Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
var. aterneum
-
Bacillus subtilis
-
168-2
-
Bacillus subtilis 168-2
-
168-2
-
Brevibacillus brevis
-
9999
-
Cyberlindnera jadinii
-
-
-
Enterobacter cloacae
-
-
-
Gluconobacter oxydans
-
Acetobacter melanogenus
-
Klebsiella aerogenes
-
-
-
no activity in Candida tropicalis
-
-
-
Priestia megaterium
-
-
-
Proteus vulgaris
-
-
-
Saccharomyces cerevisiae
-
Boehringer, baker's and brewer's yeast
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, affinity chromatography, ion exchange chromatography Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
28.4
-
-
Saccharomyces cerevisiae

Storage Stability

Storage Stability Organism
4°C, purified preparations, stable for at least 4 months Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-keto-D-fructose + NADPH
-
Saccharomyces cerevisiae L-sorbose + NADP+
-
ir
5-keto-D-fructose 1-phosphate + NADPH low activity, maximal rate of 1.8% that observed for 5-keto-D-fructose Saccharomyces cerevisiae L-sorbose 1-phosphate + NADP+
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Saccharomyces cerevisiae
7.4 8.5 Tris-acetate buffer Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
NADH
-
Gluconobacter oxydans
NADPH
-
Saccharomyces cerevisiae