BRENDA - Enzyme Database show
show all sequences of 1.1.1.123

5-keto-D-Fructose reductase from yeast

Englard, S.; Avigad, G.; Methods Enzymol. 41B, 132-138 (1975)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
NADP+
competitive inhibitor
Saccharomyces cerevisiae
p-hydroxymercuribenzoate
0.4 mM and 1 mM inhibit activity to 21% and 41% respectively
Saccharomyces cerevisiae
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.046
-
NADPH
pH 7.5, 30°C
Saccharomyces cerevisiae
0.29
-
5-Keto-D-fructose 1-phosphate
-
Saccharomyces cerevisiae
1
-
5-keto-D-fructose
pH 7.5, 30°C
Saccharomyces cerevisiae
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bacillus megaterium
-
-
-
Bacillus subtilis
-
168-2; var. aterneum
-
Bacillus subtilis 168-2
-
168-2
-
Brevibacillus brevis
-
9999
-
Cyberlindnera jadinii
-
-
-
Enterobacter aerogenes
-
-
-
Enterobacter cloacae
-
-
-
Gluconobacter oxydans
-
Acetobacter melanogenus
-
no activity in Candida tropicalis
-
-
-
Proteus vulgaris
-
-
-
Saccharomyces cerevisiae
-
Boehringer, baker's and brewer's yeast
-
Purification (Commentary)
Commentary
Organism
ammonium sulfate precipitation, affinity chromatography, ion exchange chromatography
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
28.4
-
-
Saccharomyces cerevisiae
Storage Stability
Storage Stability
Organism
4°C, purified preparations, stable for at least 4 months
Saccharomyces cerevisiae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5-keto-D-fructose + NADPH
-
285808
Saccharomyces cerevisiae
L-sorbose + NADP+
-
285808
Saccharomyces cerevisiae
ir
5-keto-D-fructose 1-phosphate + NADPH
low activity, maximal rate of 1.8% that observed for 5-keto-D-fructose
285808
Saccharomyces cerevisiae
L-sorbose 1-phosphate + NADP+
-
285808
Saccharomyces cerevisiae
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Saccharomyces cerevisiae
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
8.5
Tris-acetate buffer
Saccharomyces cerevisiae
7.4
-
assay at
Saccharomyces cerevisiae
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
-
Gluconobacter oxydans
NADPH
-
Saccharomyces cerevisiae
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
-
Gluconobacter oxydans
NADPH
-
Saccharomyces cerevisiae
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
NADP+
competitive inhibitor
Saccharomyces cerevisiae
p-hydroxymercuribenzoate
0.4 mM and 1 mM inhibit activity to 21% and 41% respectively
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.046
-
NADPH
pH 7.5, 30°C
Saccharomyces cerevisiae
0.29
-
5-Keto-D-fructose 1-phosphate
-
Saccharomyces cerevisiae
1
-
5-keto-D-fructose
pH 7.5, 30°C
Saccharomyces cerevisiae
Purification (Commentary) (protein specific)
Commentary
Organism
ammonium sulfate precipitation, affinity chromatography, ion exchange chromatography
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
28.4
-
-
Saccharomyces cerevisiae
Storage Stability (protein specific)
Storage Stability
Organism
4°C, purified preparations, stable for at least 4 months
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5-keto-D-fructose + NADPH
-
285808
Saccharomyces cerevisiae
L-sorbose + NADP+
-
285808
Saccharomyces cerevisiae
ir
5-keto-D-fructose 1-phosphate + NADPH
low activity, maximal rate of 1.8% that observed for 5-keto-D-fructose
285808
Saccharomyces cerevisiae
L-sorbose 1-phosphate + NADP+
-
285808
Saccharomyces cerevisiae
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Saccharomyces cerevisiae
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
8.5
Tris-acetate buffer
Saccharomyces cerevisiae
7.4
-
assay at
Saccharomyces cerevisiae
Other publictions for EC 1.1.1.123
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
285812
Banfield
Crystal structure of the NADP( ...
Bemisia argentifolii
J. Mol. Biol.
306
239-250
2001
-
-
-
-
-
-
-
-
-
2
1
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
1
-
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285810
Salvucci
Effect of high temperature on ...
Bemisia argentifolii
J. Insect Physiol.
45
21-27
1999
-
-
-
-
-
-
-
1
-
-
-
1
-
1
-
-
-
-
-
-
2
-
2
-
1
1
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
2
-
2
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
285811
Wolfe
Molecular basis for thermoprot ...
Bemisia argentifolii
Insect Biochem. Mol. Biol.
29
113-120
1999
-
-
1
-
1
-
-
-
-
1
4
-
-
1
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
1
4
-
-
-
-
1
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
285813
Salvucci
-
Purification and properties of ...
Bemisia argentifolii
Insect Biochem. Mol. Biol.
28
357-363
1998
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
2
-
-
1
1
2
-
-
-
2
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
1
1
2
-
-
-
2
-
-
-
-
-
-
-
-
-
285807
Yagi
-
Purification and properties of ...
Corynebacterium sp.
J. Ferment. Bioeng.
67
212-214
1989
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
-
1
-
-
-
1
-
1
1
-
1
-
-
1
1
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
1
-
-
-
-
1
-
-
1
-
1
1
-
1
-
-
1
1
-
-
-
-
-
-
-
-
285808
Englard
5-keto-D-Fructose reductase fr ...
Bacillus megaterium, Bacillus subtilis 168-2, Bacillus subtilis, Brevibacillus brevis, Cyberlindnera jadinii, Enterobacter aerogenes, Enterobacter cloacae, Gluconobacter oxydans, no activity in Candida tropicalis, Proteus vulgaris, Saccharomyces cerevisiae
Methods Enzymol.
41B
132-138
1975
-
-
-
-
-
-
2
3
-
-
-
-
-
12
-
-
1
-
-
-
1
1
2
-
1
-
-
-
2
-
-
2
-
-
-
-
-
-
2
-
-
-
-
2
-
3
-
-
-
-
-
-
-
1
-
-
1
1
2
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
285809
Englard
5-keto-D-Fructose. VI. A speci ...
Bacillus megaterium, Bacillus subtilis 168-2, Bacillus subtilis, Brevibacillus brevis, Cyberlindnera jadinii, Enterobacter aerogenes, Enterobacter cloacae, Gluconobacter cerinus, Gluconobacter oxydans, no activity in Bacillus cereus, no activity in Candida tropicalis, no activity in Escherichia coli K12, Proteus vulgaris, Saccharomyces cerevisiae
J. Biol. Chem.
245
1311-1318
1970
-
-
-
-
-
-
2
3
-
-
-
-
-
16
-
-
1
-
-
-
1
-
2
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
1
-
-
-
-
2
-
3
-
-
-
-
-
-
-
1
-
-
1
-
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-