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show all sequences of 1.1.1.123

Purification and properties of 5-keto-D-fructose reductase from a mutant strain derived from Corynebacterium sp.

Yagi, S.; Kobayahi, K.; Sonoyama, T.; J. Ferment. Bioeng. 67, 212-214 (1989)
No PubMed abstract available

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.9
-
5-keto-D-fructose
pH 7.0, 30°C
Corynebacterium sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
33000
-
gel filtration
Corynebacterium sp.
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Corynebacterium sp.
-
mutant strain, SHS 752001
-
Purification (Commentary)
Commentary
Organism
ammonium sulfate precipitation, DEAE-Sepharose-, Gel Red A-, Phenyl Sepharose column chromatography
Corynebacterium sp.
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
67.3
-
-
Corynebacterium sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5-keto-D-fructose + NADPH
-
285807
Corynebacterium sp.
L-sorbose + NADP+
-
285807
Corynebacterium sp.
ir
Subunits
Subunits
Commentary
Organism
monomer
1 * 33000, SDS-PAGE
Corynebacterium sp.
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
-
38
activity decreases gradually with increasing temperature from 0°C to 27°C, falls rapidly above 27°C and is completely lost at 38°C
Corynebacterium sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
7
-
Corynebacterium sp.
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5
9
pH 5: about 20% of activity maximum, pH 9: about 35% of activity maximum
Corynebacterium sp.
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
no activity with NADH
Corynebacterium sp.
NADPH
-
Corynebacterium sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
no activity with NADH
Corynebacterium sp.
NADPH
-
Corynebacterium sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.9
-
5-keto-D-fructose
pH 7.0, 30°C
Corynebacterium sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
33000
-
gel filtration
Corynebacterium sp.
Purification (Commentary) (protein specific)
Commentary
Organism
ammonium sulfate precipitation, DEAE-Sepharose-, Gel Red A-, Phenyl Sepharose column chromatography
Corynebacterium sp.
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
67.3
-
-
Corynebacterium sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5-keto-D-fructose + NADPH
-
285807
Corynebacterium sp.
L-sorbose + NADP+
-
285807
Corynebacterium sp.
ir
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 33000, SDS-PAGE
Corynebacterium sp.
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
-
38
activity decreases gradually with increasing temperature from 0°C to 27°C, falls rapidly above 27°C and is completely lost at 38°C
Corynebacterium sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
7
-
Corynebacterium sp.
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5
9
pH 5: about 20% of activity maximum, pH 9: about 35% of activity maximum
Corynebacterium sp.
Other publictions for EC 1.1.1.123
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
285812
Banfield
Crystal structure of the NADP( ...
Bemisia argentifolii
J. Mol. Biol.
306
239-250
2001
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2
1
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1
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1
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1
1
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2
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2
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2
1
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1
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1
1
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285810
Salvucci
Effect of high temperature on ...
Bemisia argentifolii
J. Insect Physiol.
45
21-27
1999
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1
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1
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1
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2
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2
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1
1
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1
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1
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1
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1
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2
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2
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1
1
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285811
Wolfe
Molecular basis for thermoprot ...
Bemisia argentifolii
Insect Biochem. Mol. Biol.
29
113-120
1999
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1
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1
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1
4
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1
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1
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1
1
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1
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1
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1
1
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1
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1
4
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1
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1
1
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1
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285813
Salvucci
-
Purification and properties of ...
Bemisia argentifolii
Insect Biochem. Mol. Biol.
28
357-363
1998
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2
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1
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2
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1
1
2
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2
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2
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2
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2
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2
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-
1
1
2
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-
2
-
-
-
-
-
-
-
-
-
285807
Yagi
-
Purification and properties of ...
Corynebacterium sp.
J. Ferment. Bioeng.
67
212-214
1989
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
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1
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-
-
1
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1
1
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1
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1
1
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2
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2
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1
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1
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1
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1
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1
1
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1
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1
1
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285808
Englard
5-keto-D-Fructose reductase fr ...
Bacillus megaterium, Bacillus subtilis 168-2, Bacillus subtilis, Brevibacillus brevis, Cyberlindnera jadinii, Enterobacter aerogenes, Enterobacter cloacae, Gluconobacter oxydans, no activity in Candida tropicalis, Proteus vulgaris, Saccharomyces cerevisiae
Methods Enzymol.
41B
132-138
1975
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2
3
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12
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1
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1
1
2
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1
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2
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2
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2
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2
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3
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1
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1
1
2
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1
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2
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285809
Englard
5-keto-D-Fructose. VI. A speci ...
Bacillus megaterium, Bacillus subtilis 168-2, Bacillus subtilis, Brevibacillus brevis, Cyberlindnera jadinii, Enterobacter aerogenes, Enterobacter cloacae, Gluconobacter cerinus, Gluconobacter oxydans, no activity in Bacillus cereus, no activity in Candida tropicalis, no activity in Escherichia coli K12, Proteus vulgaris, Saccharomyces cerevisiae
J. Biol. Chem.
245
1311-1318
1970
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2
3
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16
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1
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1
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2
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1
1
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1
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1
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2
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3
-
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1
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1
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2
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1
1
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