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Literature summary for 1.1.1.123 extracted from

  • Yagi, S.; Kobayahi, K.; Sonoyama, T.
    Purification and properties of 5-keto-D-fructose reductase from a mutant strain derived from Corynebacterium sp. (1989), J. Ferment. Bioeng., 67, 212-214.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.9
-
5-keto-D-fructose pH 7.0, 30°C Corynebacterium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
gel filtration Corynebacterium sp.

Organism

Organism UniProt Comment Textmining
Corynebacterium sp.
-
mutant strain, SHS 752001
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, DEAE-Sepharose-, Gel Red A-, Phenyl Sepharose column chromatography Corynebacterium sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
67.3
-
-
Corynebacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-keto-D-fructose + NADPH
-
Corynebacterium sp. L-sorbose + NADP+
-
ir

Subunits

Subunits Comment Organism
monomer 1 * 33000, SDS-PAGE Corynebacterium sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
-
38 activity decreases gradually with increasing temperature from 0°C to 27°C, falls rapidly above 27°C and is completely lost at 38°C Corynebacterium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7
-
Corynebacterium sp.

pH Range

pH Minimum pH Maximum Comment Organism
5 9 pH 5: about 20% of activity maximum, pH 9: about 35% of activity maximum Corynebacterium sp.

Cofactor

Cofactor Comment Organism Structure
additional information no activity with NADH Corynebacterium sp.
NADPH
-
Corynebacterium sp.