BRENDA - Enzyme Database show
show all sequences of 1.1.1.122

Isolation of diphosphopyridine nucleotide-dependent L-fucose dehydrogenase from pork liver

Schacter, H.; Sarney, J.; McGuire, E.J.; Roseman, S.; J. Biol. Chem. 244, 4785-4792 (1969)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
hydroxylamine
stimulation at high concentrations
Sus scrofa
Inhibitors
Inhibitors
Commentary
Organism
Structure
3-Amino-3-deoxy-D-arabinose
-
Sus scrofa
D-arabinose
competitive inhibition of L-fucose oxidation at 2.5 mM
Sus scrofa
p-hydroxymercuribenzoate
20% loss of activity, saturated
Sus scrofa
sodium iodoacetate
20% loss of activity at 20 mM, pH 8.0
Sus scrofa
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.02
-
NAD+
-
Sus scrofa
0.32
-
6-deoxy-L-galactose
-
Sus scrofa
2.1
-
D-arabinose
-
Sus scrofa
8
-
3-amino-D-arabinose
-
Sus scrofa
8
-
L-galactose
-
Sus scrofa
44
-
L-Xylose
-
Sus scrofa
48
-
D-Lyxose
-
Sus scrofa
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3-amino-3-deoxy-D-arabinose + NAD+
Sus scrofa
15% of the activity compared to L-fucose
3-amino-3-deoxy-D-arabino-1,5-lactone + NADH
-
Sus scrofa
?
6-deoxy-L-galactose + NAD+
Sus scrofa
i.e. L-fucose
6-deoxy-L-galactono-1,5-lactone + NADH
-
Sus scrofa
?
D-arabinose + NAD+
Sus scrofa
66% of the activity compared to L-fucose
D-arabinono-1,4-lactone + NADH
-
Sus scrofa
?
D-lyxose + NAD+
Sus scrofa
4% of the activity compared to L-fucose
? + NADH
-
Sus scrofa
?
L-galactose + NAD+
Sus scrofa
13% of the activity compared to L-fucose
L-galactono-1,5-lactone + NADH
-
Sus scrofa
?
L-xylose + NAD+
Sus scrofa
4% of the activity compared to L-fucose
L-xylono-1,5-lactone + NADH
-
Sus scrofa
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Sus scrofa
-
porcine
-
Purification (Commentary)
Commentary
Organism
to homogeneity, chromatography steps, preparative gel electrophoresis
Sus scrofa
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Sus scrofa
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
433
-
purified enzyme
Sus scrofa
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-amino-3-deoxy-D-arabinose + NAD+
15% of the activity compared to L-fucose
285806
Sus scrofa
3-amino-3-deoxy-D-arabino-1,5-lactone + NADH
-
285806
Sus scrofa
?
6-deoxy-L-galactose + NAD+
i.e. L-fucose
285806
Sus scrofa
6-deoxy-L-galactono-1,5-lactone + NADH
-
285806
Sus scrofa
?
D-arabinose + NAD+
66% of the activity compared to L-fucose
285806
Sus scrofa
D-arabinono-1,4-lactone + NADH
-
285806
Sus scrofa
?
D-lyxose + NAD+
4% of the activity compared to L-fucose
285806
Sus scrofa
? + NADH
-
285806
Sus scrofa
?
L-galactose + NAD+
13% of the activity compared to L-fucose
285806
Sus scrofa
L-galactono-1,5-lactone + NADH
-
285806
Sus scrofa
?
L-xylose + NAD+
4% of the activity compared to L-fucose
285806
Sus scrofa
L-xylono-1,5-lactone + NADH
-
285806
Sus scrofa
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.7
-
-
Sus scrofa
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
NAD-specific
Sus scrofa
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
hydroxylamine
stimulation at high concentrations
Sus scrofa
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
NAD-specific
Sus scrofa
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
3-Amino-3-deoxy-D-arabinose
-
Sus scrofa
D-arabinose
competitive inhibition of L-fucose oxidation at 2.5 mM
Sus scrofa
p-hydroxymercuribenzoate
20% loss of activity, saturated
Sus scrofa
sodium iodoacetate
20% loss of activity at 20 mM, pH 8.0
Sus scrofa
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.02
-
NAD+
-
Sus scrofa
0.32
-
6-deoxy-L-galactose
-
Sus scrofa
2.1
-
D-arabinose
-
Sus scrofa
8
-
3-amino-D-arabinose
-
Sus scrofa
8
-
L-galactose
-
Sus scrofa
44
-
L-Xylose
-
Sus scrofa
48
-
D-Lyxose
-
Sus scrofa
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3-amino-3-deoxy-D-arabinose + NAD+
Sus scrofa
15% of the activity compared to L-fucose
3-amino-3-deoxy-D-arabino-1,5-lactone + NADH
-
Sus scrofa
?
6-deoxy-L-galactose + NAD+
Sus scrofa
i.e. L-fucose
6-deoxy-L-galactono-1,5-lactone + NADH
-
Sus scrofa
?
D-arabinose + NAD+
Sus scrofa
66% of the activity compared to L-fucose
D-arabinono-1,4-lactone + NADH
-
Sus scrofa
?
D-lyxose + NAD+
Sus scrofa
4% of the activity compared to L-fucose
? + NADH
-
Sus scrofa
?
L-galactose + NAD+
Sus scrofa
13% of the activity compared to L-fucose
L-galactono-1,5-lactone + NADH
-
Sus scrofa
?
L-xylose + NAD+
Sus scrofa
4% of the activity compared to L-fucose
L-xylono-1,5-lactone + NADH
-
Sus scrofa
?
Purification (Commentary) (protein specific)
Commentary
Organism
to homogeneity, chromatography steps, preparative gel electrophoresis
Sus scrofa
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Sus scrofa
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
433
-
purified enzyme
Sus scrofa
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-amino-3-deoxy-D-arabinose + NAD+
15% of the activity compared to L-fucose
285806
Sus scrofa
3-amino-3-deoxy-D-arabino-1,5-lactone + NADH
-
285806
Sus scrofa
?
6-deoxy-L-galactose + NAD+
i.e. L-fucose
285806
Sus scrofa
6-deoxy-L-galactono-1,5-lactone + NADH
-
285806
Sus scrofa
?
D-arabinose + NAD+
66% of the activity compared to L-fucose
285806
Sus scrofa
D-arabinono-1,4-lactone + NADH
-
285806
Sus scrofa
?
D-lyxose + NAD+
4% of the activity compared to L-fucose
285806
Sus scrofa
? + NADH
-
285806
Sus scrofa
?
L-galactose + NAD+
13% of the activity compared to L-fucose
285806
Sus scrofa
L-galactono-1,5-lactone + NADH
-
285806
Sus scrofa
?
L-xylose + NAD+
4% of the activity compared to L-fucose
285806
Sus scrofa
L-xylono-1,5-lactone + NADH
-
285806
Sus scrofa
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.7
-
-
Sus scrofa
Other publictions for EC 1.1.1.122
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
681445
Brouns
Crystal structure and biochemi ...
Sulfolobus solfataricus, Sulfolobus solfataricus P2
J. Mol. Biol.
371
1249-1260
2007
-
-
-
1
-
-
-
-
-
1
-
-
-
3
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
719837
Brouns
Identification of the missing ...
Sulfolobus solfataricus, Sulfolobus solfataricus P2
J. Biol. Chem.
281
27378-27388
2006
-
-
1
-
-
-
-
-
-
1
2
-
-
3
-
-
-
-
-
-
-
-
2
1
1
-
2
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
2
1
1
-
2
-
1
-
-
-
-
-
-
-
-
-
655086
Bakke
-
Improvement in thermal stabili ...
Pseudomonas sp., Pseudomonas sp. No. 1143
Biotechnol. Lett.
24
1075-1078
2002
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
-
-
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2
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2
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1
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1
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1
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-
-
-
-
-
-
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
285798
Itoh
-
Purification and characterizat ...
Agrobacterium tumefaciens, Agrobacterium tumefaciens K-28
J. Ferment. Bioeng.
77
100-102
1994
-
-
-
-
-
-
4
5
-
-
1
6
-
2
-
-
1
-
-
1
2
-
8
-
1
-
1
-
1
-
1
1
-
-
-
-
-
-
1
-
-
-
-
4
-
5
-
-
1
6
-
-
-
1
-
1
2
-
8
-
1
-
1
-
1
-
1
-
-
-
-
-
-
-
285799
Yamamoto-Otake
Cloning and sequencing of the ...
Pseudomonas sp.
Biosci. Biotechnol. Biochem.
58
2281-2282
1994
-
-
1
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
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-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285800
Tsuji
Purification and some properti ...
Agrobacterium tumefaciens
Biochim. Biophys. Acta
1117
167-173
1992
-
1
-
-
-
1
4
4
-
-
3
3
-
4
-
-
1
-
-
1
1
1
4
1
-
-
1
-
-
1
1
2
-
-
-
-
1
-
2
-
-
1
-
4
-
4
-
-
3
3
-
-
-
1
-
1
1
1
4
1
-
-
1
-
-
1
1
-
-
-
-
-
-
-
285801
Guimaraes
-
Purification and general prope ...
Aureobasidium pullulans
Arq. Biol. Tecnol. (Curitiba)
32
575-587
1989
-
-
-
-
-
-
2
4
-
-
1
3
-
1
-
-
1
-
-
1
-
1
3
1
-
-
1
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
2
-
4
-
-
1
3
-
-
-
1
-
1
-
1
3
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
285802
Sasajima
Oxidation of L-glucose by a ps ...
Paraburkholderia caryophylli
Biochim. Biophys. Acta
571
120-126
1979
-
-
-
-
-
1
4
4
-
-
1
7
-
1
-
-
1
-
-
1
1
1
7
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
1
-
4
-
4
-
-
1
7
-
-
-
1
-
1
1
1
7
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
285805
Endo
Isolation and characterization ...
Oryctolagus cuniculus
J. Biochem.
86
1559-1565
1979
-
-
-
-
-
-
4
3
-
-
1
4
-
2
-
-
1
-
-
2
1
1
4
-
1
-
1
-
1
-
1
1
-
-
-
-
-
-
1
-
-
-
-
4
-
3
-
-
1
4
-
-
-
1
-
2
1
1
4
-
1
-
1
-
1
-
1
-
-
-
-
-
-
-
285803
Mobley
The physical properties of NAD ...
Ovis aries aries
Arch. Biochem. Biophys.
186
184-188
1978
5
-
-
-
-
1
3
1
-
-
3
2
-
2
-
-
1
-
-
2
-
1
2
1
-
-
2
-
-
-
-
1
-
-
-
5
-
-
1
-
-
1
-
3
-
1
-
-
3
2
-
-
-
1
-
2
-
1
2
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
285804
Mobley
L-Fucose dehydrogenase from sh ...
Ovis aries aries
Methods Enzymol.
41B
173-177
1975
-
-
-
-
-
1
-
6
-
-
1
4
-
2
-
-
1
-
-
2
-
1
4
-
-
-
1
-
1
-
-
1
-
-
-
-
-
-
1
-
-
1
-
-
-
6
-
-
1
4
-
-
-
1
-
2
-
1
4
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
285806
Schacter
Isolation of diphosphopyridine ...
Sus scrofa
J. Biol. Chem.
244
4785-4792
1969
1
-
-
-
-
-
4
7
-
-
-
6
-
2
-
-
1
-
-
2
1
-
6
-
-
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-
-
1
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1
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1
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1
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4
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7
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6
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1
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2
1
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6
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1
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