BRENDA - Enzyme Database show
show all sequences of 1.1.1.122

L-Fucose dehydrogenase from sheep liver

Mobley, P.W.; Metzger, R.P.; Wick, A.N.; Methods Enzymol. 41B, 173-177 (1975)

Data extracted from this reference:

General Stability
General Stability
Organism
stable to refrigeration at 5°C for a week
Ovis aries aries
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.01
-
NAD+
at pH 10.4
Ovis aries aries
0.04
-
NAD+
at pH 8.6
Ovis aries aries
0.07
-
6-deoxy-L-galactose
at pH 8.6
Ovis aries aries
0.4
-
D-arabinose
at pH 8.6
Ovis aries aries
1.5
-
6-deoxy-L-galactose
at pH 10.4
Ovis aries aries
7.3
-
D-arabinose
at pH 10.4
Ovis aries aries
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
96000
-
electro focusing
Ovis aries aries
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2-deoxy-D-ribose + NAD+
Ovis aries aries
10% of the activity compared to L-fucose or D-arabinose
? + NADH
-
Ovis aries aries
?
6-deoxy-L-galactose + NAD+
Ovis aries aries
ordered bi-bi-reaction mechanism
6-deoxy-L-galactono-1,5-lactone + NADH
-
Ovis aries aries
?
6-deoxy-L-galactose + NAD+
Ovis aries aries
i.e. L-fucose
6-deoxy-L-galactono-1,5-lactone + NADH
-
Ovis aries aries
?
D-arabinose + NAD+
Ovis aries aries
-
D-arabinono-1,4-lactone + NADH
-
Ovis aries aries
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Ovis aries aries
-
sheep
-
Purification (Commentary)
Commentary
Organism
to homogeneity, chromatography techniques
Ovis aries aries
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Ovis aries aries
-
Storage Stability
Storage Stability
Organism
-20°C, minimum concentration: 1 mg/ml protein, 1 month
Ovis aries aries
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-deoxy-D-ribose + NAD+
10% of the activity compared to L-fucose or D-arabinose
285804
Ovis aries aries
? + NADH
-
285804
Ovis aries aries
?
6-deoxy-L-galactose + NAD+
ordered bi-bi-reaction mechanism
285804
Ovis aries aries
6-deoxy-L-galactono-1,5-lactone + NADH
-
285804
Ovis aries aries
?
6-deoxy-L-galactose + NAD+
i.e. L-fucose
285804
Ovis aries aries
6-deoxy-L-galactono-1,5-lactone + NADH
-
285804
Ovis aries aries
?
D-arabinose + NAD+
-
285804
Ovis aries aries
D-arabinono-1,4-lactone + NADH
-
285804
Ovis aries aries
?
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
60
-
stable up to
Ovis aries aries
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
10.4
-
ionic strength: 0.05
Ovis aries aries
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Ovis aries aries
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Ovis aries aries
General Stability (protein specific)
General Stability
Organism
stable to refrigeration at 5°C for a week
Ovis aries aries
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.01
-
NAD+
at pH 10.4
Ovis aries aries
0.04
-
NAD+
at pH 8.6
Ovis aries aries
0.07
-
6-deoxy-L-galactose
at pH 8.6
Ovis aries aries
0.4
-
D-arabinose
at pH 8.6
Ovis aries aries
1.5
-
6-deoxy-L-galactose
at pH 10.4
Ovis aries aries
7.3
-
D-arabinose
at pH 10.4
Ovis aries aries
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
96000
-
electro focusing
Ovis aries aries
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2-deoxy-D-ribose + NAD+
Ovis aries aries
10% of the activity compared to L-fucose or D-arabinose
? + NADH
-
Ovis aries aries
?
6-deoxy-L-galactose + NAD+
Ovis aries aries
ordered bi-bi-reaction mechanism
6-deoxy-L-galactono-1,5-lactone + NADH
-
Ovis aries aries
?
6-deoxy-L-galactose + NAD+
Ovis aries aries
i.e. L-fucose
6-deoxy-L-galactono-1,5-lactone + NADH
-
Ovis aries aries
?
D-arabinose + NAD+
Ovis aries aries
-
D-arabinono-1,4-lactone + NADH
-
Ovis aries aries
?
Purification (Commentary) (protein specific)
Commentary
Organism
to homogeneity, chromatography techniques
Ovis aries aries
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Ovis aries aries
-
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, minimum concentration: 1 mg/ml protein, 1 month
Ovis aries aries
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-deoxy-D-ribose + NAD+
10% of the activity compared to L-fucose or D-arabinose
285804
Ovis aries aries
? + NADH
-
285804
Ovis aries aries
?
6-deoxy-L-galactose + NAD+
ordered bi-bi-reaction mechanism
285804
Ovis aries aries
6-deoxy-L-galactono-1,5-lactone + NADH
-
285804
Ovis aries aries
?
6-deoxy-L-galactose + NAD+
i.e. L-fucose
285804
Ovis aries aries
6-deoxy-L-galactono-1,5-lactone + NADH
-
285804
Ovis aries aries
?
D-arabinose + NAD+
-
285804
Ovis aries aries
D-arabinono-1,4-lactone + NADH
-
285804
Ovis aries aries
?
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
60
-
stable up to
Ovis aries aries
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
10.4
-
ionic strength: 0.05
Ovis aries aries
Other publictions for EC 1.1.1.122
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
681445
Brouns
Crystal structure and biochemi ...
Sulfolobus solfataricus, Sulfolobus solfataricus P2
J. Mol. Biol.
371
1249-1260
2007
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-
-
1
-
-
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1
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-
-
3
-
-
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2
1
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1
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1
1
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1
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-
2
1
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-
-
-
-
-
-
-
-
-
-
-
-
-
719837
Brouns
Identification of the missing ...
Sulfolobus solfataricus, Sulfolobus solfataricus P2
J. Biol. Chem.
281
27378-27388
2006
-
-
1
-
-
-
-
-
-
1
2
-
-
3
-
-
-
-
-
-
-
-
2
1
1
-
2
-
1
-
-
1
-
-
-
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1
1
-
-
-
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-
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1
2
-
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-
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2
1
1
-
2
-
1
-
-
-
-
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-
-
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-
655086
Bakke
-
Improvement in thermal stabili ...
Pseudomonas sp., Pseudomonas sp. No. 1143
Biotechnol. Lett.
24
1075-1078
2002
-
-
-
-
-
1
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2
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2
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2
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1
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1
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1
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2
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-
2
-
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-
-
-
-
-
-
-
285798
Itoh
-
Purification and characterizat ...
Agrobacterium tumefaciens, Agrobacterium tumefaciens K-28
J. Ferment. Bioeng.
77
100-102
1994
-
-
-
-
-
-
4
5
-
-
1
6
-
2
-
-
1
-
-
1
2
-
8
-
1
-
1
-
1
-
1
1
-
-
-
-
-
-
1
-
-
-
-
4
-
5
-
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1
6
-
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1
-
1
2
-
8
-
1
-
1
-
1
-
1
-
-
-
-
-
-
-
285799
Yamamoto-Otake
Cloning and sequencing of the ...
Pseudomonas sp.
Biosci. Biotechnol. Biochem.
58
2281-2282
1994
-
-
1
-
-
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-
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1
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3
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1
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1
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1
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1
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-
285800
Tsuji
Purification and some properti ...
Agrobacterium tumefaciens
Biochim. Biophys. Acta
1117
167-173
1992
-
1
-
-
-
1
4
4
-
-
3
3
-
4
-
-
1
-
-
1
1
1
4
1
-
-
1
-
-
1
1
2
-
-
-
-
1
-
2
-
-
1
-
4
-
4
-
-
3
3
-
-
-
1
-
1
1
1
4
1
-
-
1
-
-
1
1
-
-
-
-
-
-
-
285801
Guimaraes
-
Purification and general prope ...
Aureobasidium pullulans
Arq. Biol. Tecnol. (Curitiba)
32
575-587
1989
-
-
-
-
-
-
2
4
-
-
1
3
-
1
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1
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1
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1
3
1
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1
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1
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1
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1
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2
-
4
-
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1
3
-
-
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1
-
1
-
1
3
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
285802
Sasajima
Oxidation of L-glucose by a ps ...
Paraburkholderia caryophylli
Biochim. Biophys. Acta
571
120-126
1979
-
-
-
-
-
1
4
4
-
-
1
7
-
1
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1
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1
1
1
7
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1
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2
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2
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1
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4
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4
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1
7
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1
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1
1
1
7
-
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1
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-
-
-
-
-
-
-
-
285805
Endo
Isolation and characterization ...
Oryctolagus cuniculus
J. Biochem.
86
1559-1565
1979
-
-
-
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4
3
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1
4
-
2
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1
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2
1
1
4
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1
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1
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1
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1
1
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1
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4
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3
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1
4
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1
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2
1
1
4
-
1
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1
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1
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1
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-
-
-
-
-
-
285803
Mobley
The physical properties of NAD ...
Ovis aries aries
Arch. Biochem. Biophys.
186
184-188
1978
5
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1
3
1
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3
2
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2
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1
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2
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1
2
1
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2
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1
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5
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1
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1
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3
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1
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3
2
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1
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2
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1
2
1
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-
2
-
-
-
-
-
-
-
-
-
-
-
285804
Mobley
L-Fucose dehydrogenase from sh ...
Ovis aries aries
Methods Enzymol.
41B
173-177
1975
-
-
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-
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1
-
6
-
-
1
4
-
2
-
-
1
-
-
2
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1
4
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1
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1
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1
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1
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1
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6
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1
4
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1
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2
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1
4
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1
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1
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285806
Schacter
Isolation of diphosphopyridine ...
Sus scrofa
J. Biol. Chem.
244
4785-4792
1969
1
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4
7
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6
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