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show all sequences of 1.1.1.122

The physical properties of NAD-dependent L-fucose dehydrogenase from sheep liver

Mobley, P.W.; Metzger, R.P.; Arch. Biochem. Biophys. 186, 184-188 (1978)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
KNO3
activation at 1-1.3 M, optimal pH shifts to 9-9.5
Ovis aries aries
NaBr
activation at 1-1.3 M, optimal pH shifts to 9-9.5
Ovis aries aries
NaCl
activation at 1-1.3 M, optimal pH shifts to 9-9.5
Ovis aries aries
NaNO3
activation at 1-1.3 M, optimal pH shifts to 9-9.5
Ovis aries aries
Tris-HCl
activation at 1-1.3 M, optimal pH shifts to 9-9.5
Ovis aries aries
General Stability
General Stability
Organism
NAD+ stabilizes
Ovis aries aries
Inhibitors
Inhibitors
Commentary
Organism
Structure
ADP
slight inhibition
Ovis aries aries
ATP
slight inhibition
Ovis aries aries
NADH
Ki: 0.04 mM
Ovis aries aries
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.2
-
L-galactose
-
Ovis aries aries
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
30000
-
x * 30000, SDS-PAGE, x * 35000, guanidinium-HCl gel filtration
Ovis aries aries
35000
-
x * 30000, SDS-PAGE, x * 35000, guanidinium-HCl gel filtration
Ovis aries aries
82000
123000
sedimentation equilibrium analysis: 123000 Da, gel filtration: 82000 Da
Ovis aries aries
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6-deoxy-L-galactose + NAD+
Ovis aries aries
i.e. L-fucose
6-deoxy-L-galactono-1,5-lactone + NADH
-
Ovis aries aries
?
L-galactose + NAD+
Ovis aries aries
-
L-galactono-1,5-lactone + NADH
-
Ovis aries aries
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Ovis aries aries
-
sheep
-
Purification (Commentary)
Commentary
Organism
to homogeneity, chromatography techniques
Ovis aries aries
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Ovis aries aries
-
Storage Stability
Storage Stability
Organism
4C, pH 7.2, half-life: 39 days
Ovis aries aries
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6-deoxy-L-galactose + NAD+
i.e. L-fucose
285803
Ovis aries aries
6-deoxy-L-galactono-1,5-lactone + NADH
-
285803
Ovis aries aries
?
L-galactose + NAD+
-
285803
Ovis aries aries
L-galactono-1,5-lactone + NADH
-
285803
Ovis aries aries
?
Subunits
Subunits
Commentary
Organism
?
x * 30000, SDS-PAGE, x * 35000, guanidinium-HCl gel filtration
Ovis aries aries
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
25
-
at pH 7.2: half-life: 1.8 days
Ovis aries aries
60
-
at pH 7.2, half-life: 25 min without added NAD+, half-life: 35 min including added NAD+, at pH 8.5: activity more rapidly lost
Ovis aries aries
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Ovis aries aries
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
KNO3
activation at 1-1.3 M, optimal pH shifts to 9-9.5
Ovis aries aries
NaBr
activation at 1-1.3 M, optimal pH shifts to 9-9.5
Ovis aries aries
NaCl
activation at 1-1.3 M, optimal pH shifts to 9-9.5
Ovis aries aries
NaNO3
activation at 1-1.3 M, optimal pH shifts to 9-9.5
Ovis aries aries
Tris-HCl
activation at 1-1.3 M, optimal pH shifts to 9-9.5
Ovis aries aries
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Ovis aries aries
General Stability (protein specific)
General Stability
Organism
NAD+ stabilizes
Ovis aries aries
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
ADP
slight inhibition
Ovis aries aries
ATP
slight inhibition
Ovis aries aries
NADH
Ki: 0.04 mM
Ovis aries aries
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.2
-
L-galactose
-
Ovis aries aries
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
30000
-
x * 30000, SDS-PAGE, x * 35000, guanidinium-HCl gel filtration
Ovis aries aries
35000
-
x * 30000, SDS-PAGE, x * 35000, guanidinium-HCl gel filtration
Ovis aries aries
82000
123000
sedimentation equilibrium analysis: 123000 Da, gel filtration: 82000 Da
Ovis aries aries
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6-deoxy-L-galactose + NAD+
Ovis aries aries
i.e. L-fucose
6-deoxy-L-galactono-1,5-lactone + NADH
-
Ovis aries aries
?
L-galactose + NAD+
Ovis aries aries
-
L-galactono-1,5-lactone + NADH
-
Ovis aries aries
?
Purification (Commentary) (protein specific)
Commentary
Organism
to homogeneity, chromatography techniques
Ovis aries aries
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Ovis aries aries
-
Storage Stability (protein specific)
Storage Stability
Organism
4C, pH 7.2, half-life: 39 days
Ovis aries aries
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6-deoxy-L-galactose + NAD+
i.e. L-fucose
285803
Ovis aries aries
6-deoxy-L-galactono-1,5-lactone + NADH
-
285803
Ovis aries aries
?
L-galactose + NAD+
-
285803
Ovis aries aries
L-galactono-1,5-lactone + NADH
-
285803
Ovis aries aries
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 30000, SDS-PAGE, x * 35000, guanidinium-HCl gel filtration
Ovis aries aries
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
25
-
at pH 7.2: half-life: 1.8 days
Ovis aries aries
60
-
at pH 7.2, half-life: 25 min without added NAD+, half-life: 35 min including added NAD+, at pH 8.5: activity more rapidly lost
Ovis aries aries
Other publictions for EC 1.1.1.122
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
681445
Brouns
Crystal structure and biochemi ...
Sulfolobus solfataricus, Sulfolobus solfataricus P2
J. Mol. Biol.
371
1249-1260
2007
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1
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1
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3
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1
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1
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1
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1
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1
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719837
Brouns
Identification of the missing ...
Sulfolobus solfataricus, Sulfolobus solfataricus P2
J. Biol. Chem.
281
27378-27388
2006
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-
1
-
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1
2
-
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3
-
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2
1
1
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2
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1
-
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1
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1
1
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-
-
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1
2
-
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-
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2
1
1
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2
-
1
-
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-
-
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655086
Bakke
-
Improvement in thermal stabili ...
Pseudomonas sp., Pseudomonas sp. No. 1143
Biotechnol. Lett.
24
1075-1078
2002
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-
-
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1
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2
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2
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2
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1
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1
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1
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2
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2
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285798
Itoh
-
Purification and characterizat ...
Agrobacterium tumefaciens, Agrobacterium tumefaciens K-28
J. Ferment. Bioeng.
77
100-102
1994
-
-
-
-
-
-
4
5
-
-
1
6
-
2
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-
1
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-
1
2
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8
-
1
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1
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1
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1
1
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1
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4
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5
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1
6
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1
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1
2
-
8
-
1
-
1
-
1
-
1
-
-
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-
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-
285799
Yamamoto-Otake
Cloning and sequencing of the ...
Pseudomonas sp.
Biosci. Biotechnol. Biochem.
58
2281-2282
1994
-
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1
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1
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285800
Tsuji
Purification and some properti ...
Agrobacterium tumefaciens
Biochim. Biophys. Acta
1117
167-173
1992
-
1
-
-
-
1
4
4
-
-
3
3
-
4
-
-
1
-
-
1
1
1
4
1
-
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1
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-
1
1
2
-
-
-
-
1
-
2
-
-
1
-
4
-
4
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-
3
3
-
-
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1
-
1
1
1
4
1
-
-
1
-
-
1
1
-
-
-
-
-
-
-
285801
Guimaraes
-
Purification and general prope ...
Aureobasidium pullulans
Arq. Biol. Tecnol. (Curitiba)
32
575-587
1989
-
-
-
-
-
-
2
4
-
-
1
3
-
1
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1
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1
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1
3
1
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1
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1
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1
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1
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2
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4
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1
3
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1
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1
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1
3
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
285802
Sasajima
Oxidation of L-glucose by a ps ...
Paraburkholderia caryophylli
Biochim. Biophys. Acta
571
120-126
1979
-
-
-
-
-
1
4
4
-
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1
7
-
1
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1
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1
1
1
7
-
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1
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2
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2
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1
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4
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4
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1
7
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1
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1
1
1
7
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1
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285805
Endo
Isolation and characterization ...
Oryctolagus cuniculus
J. Biochem.
86
1559-1565
1979
-
-
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4
3
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1
4
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2
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1
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4
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1
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1
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1
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1
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1
1
4
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1
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1
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1
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1
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285803
Mobley
The physical properties of NAD ...
Ovis aries aries
Arch. Biochem. Biophys.
186
184-188
1978
5
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-
-
1
3
1
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3
2
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2
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1
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2
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1
2
1
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2
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1
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2
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1
2
1
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2
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285804
Mobley
L-Fucose dehydrogenase from sh ...
Ovis aries aries
Methods Enzymol.
41B
173-177
1975
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1
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6
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1
4
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2
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1
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1
4
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1
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1
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1
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1
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285806
Schacter
Isolation of diphosphopyridine ...
Sus scrofa
J. Biol. Chem.
244
4785-4792
1969
1
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