BRENDA - Enzyme Database show
show all sequences of 1.1.1.122

Purification and characterization of L-fucose dehydrogenase from Agrobacterium tumefaciens K-28

Itoh, H.; Miyoshi, T.; Yoshino, A.; Shiragami, K.; Izumori, K.; J. Ferment. Bioeng. 77, 100-102 (1994)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Cu2+
80% inhibition
Agrobacterium tumefaciens
Hg2+
complete inhibition
Agrobacterium tumefaciens
p-chloromercuribenzoate
90% inhibition
Agrobacterium tumefaciens
Zn2+
40% inhibition
Agrobacterium tumefaciens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.6
-
NADP+
-
Agrobacterium tumefaciens
1.89
-
NAD+
-
Agrobacterium tumefaciens
11.1
-
D-galactose
-
Agrobacterium tumefaciens
12.8
-
6-deoxy-L-galactose
-
Agrobacterium tumefaciens
34.4
-
D-arabinose
-
Agrobacterium tumefaciens
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
100000
-
gel filtration
Agrobacterium tumefaciens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6-deoxy-L-galactose + NAD+
Agrobacterium tumefaciens
i.e. L-fucose
6-deoxy-L-galactono-1,5-lactone + NADH
-
Agrobacterium tumefaciens
?
6-deoxy-L-galactose + NAD+
Agrobacterium tumefaciens K-28
i.e. L-fucose
6-deoxy-L-galactono-1,5-lactone + NADH
-
Agrobacterium tumefaciens K-28
?
D-arabinose + NAD+
Agrobacterium tumefaciens
74.5% of the activity compared to L-fucose
D-arabinono-1,4-lactone + NADH
-
Agrobacterium tumefaciens
?
D-arabinose + NAD+
Agrobacterium tumefaciens K-28
74.5% of the activity compared to L-fucose
D-arabinono-1,4-lactone + NADH
-
Agrobacterium tumefaciens K-28
?
L-galactose + NAD+
Agrobacterium tumefaciens
59.6% of the activity compared to L-fucose
L-galactono-1,5-lactone + NADH
-
Agrobacterium tumefaciens
?
L-galactose + NAD+
Agrobacterium tumefaciens K-28
59.6% of the activity compared to L-fucose
L-galactono-1,5-lactone + NADH
-
Agrobacterium tumefaciens K-28
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Agrobacterium tumefaciens
-
strain K-28
-
Agrobacterium tumefaciens K-28
-
strain K-28
-
Purification (Commentary)
Commentary
Organism
to homogeneity, chromatography techniques
Agrobacterium tumefaciens
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Agrobacterium tumefaciens
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.88
-
crude enzyme, cells grown in sucrose-containing medium
Agrobacterium tumefaciens
375
-
purified enzyme
Agrobacterium tumefaciens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6-deoxy-L-galactose + NAD+
i.e. L-fucose
285798
Agrobacterium tumefaciens
6-deoxy-L-galactono-1,5-lactone + NADH
-
285798
Agrobacterium tumefaciens
?
6-deoxy-L-galactose + NAD+
i.e. L-fucose
285798
Agrobacterium tumefaciens K-28
6-deoxy-L-galactono-1,5-lactone + NADH
-
285798
Agrobacterium tumefaciens K-28
?
D-arabinose + NAD+
74.5% of the activity compared to L-fucose
285798
Agrobacterium tumefaciens
D-arabinono-1,4-lactone + NADH
-
285798
Agrobacterium tumefaciens
?
D-arabinose + NAD+
74.5% of the activity compared to L-fucose
285798
Agrobacterium tumefaciens K-28
D-arabinono-1,4-lactone + NADH
-
285798
Agrobacterium tumefaciens K-28
?
fuconolactone + NADPH + H+
-
285798
Agrobacterium tumefaciens
L-fucose + NADP+
-
-
-
-
fuconolactone + NADPH + H+
-
285798
Agrobacterium tumefaciens K-28
L-fucose + NADP+
-
-
-
-
L-galactose + NAD+
59.6% of the activity compared to L-fucose
285798
Agrobacterium tumefaciens
L-galactono-1,5-lactone + NADH
-
285798
Agrobacterium tumefaciens
?
L-galactose + NAD+
59.6% of the activity compared to L-fucose
285798
Agrobacterium tumefaciens K-28
L-galactono-1,5-lactone + NADH
-
285798
Agrobacterium tumefaciens K-28
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
50
-
Agrobacterium tumefaciens
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
40
-
stable at
Agrobacterium tumefaciens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
10
-
Agrobacterium tumefaciens
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
7.5
9
stable within
Agrobacterium tumefaciens
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
also able to use NADP+
Agrobacterium tumefaciens
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
also able to use NADP+
Agrobacterium tumefaciens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Cu2+
80% inhibition
Agrobacterium tumefaciens
Hg2+
complete inhibition
Agrobacterium tumefaciens
p-chloromercuribenzoate
90% inhibition
Agrobacterium tumefaciens
Zn2+
40% inhibition
Agrobacterium tumefaciens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.6
-
NADP+
-
Agrobacterium tumefaciens
1.89
-
NAD+
-
Agrobacterium tumefaciens
11.1
-
D-galactose
-
Agrobacterium tumefaciens
12.8
-
6-deoxy-L-galactose
-
Agrobacterium tumefaciens
34.4
-
D-arabinose
-
Agrobacterium tumefaciens
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
100000
-
gel filtration
Agrobacterium tumefaciens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6-deoxy-L-galactose + NAD+
Agrobacterium tumefaciens
i.e. L-fucose
6-deoxy-L-galactono-1,5-lactone + NADH
-
Agrobacterium tumefaciens
?
6-deoxy-L-galactose + NAD+
Agrobacterium tumefaciens K-28
i.e. L-fucose
6-deoxy-L-galactono-1,5-lactone + NADH
-
Agrobacterium tumefaciens K-28
?
D-arabinose + NAD+
Agrobacterium tumefaciens
74.5% of the activity compared to L-fucose
D-arabinono-1,4-lactone + NADH
-
Agrobacterium tumefaciens
?
D-arabinose + NAD+
Agrobacterium tumefaciens K-28
74.5% of the activity compared to L-fucose
D-arabinono-1,4-lactone + NADH
-
Agrobacterium tumefaciens K-28
?
L-galactose + NAD+
Agrobacterium tumefaciens
59.6% of the activity compared to L-fucose
L-galactono-1,5-lactone + NADH
-
Agrobacterium tumefaciens
?
L-galactose + NAD+
Agrobacterium tumefaciens K-28
59.6% of the activity compared to L-fucose
L-galactono-1,5-lactone + NADH
-
Agrobacterium tumefaciens K-28
?
Purification (Commentary) (protein specific)
Commentary
Organism
to homogeneity, chromatography techniques
Agrobacterium tumefaciens
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Agrobacterium tumefaciens
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.88
-
crude enzyme, cells grown in sucrose-containing medium
Agrobacterium tumefaciens
375
-
purified enzyme
Agrobacterium tumefaciens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6-deoxy-L-galactose + NAD+
i.e. L-fucose
285798
Agrobacterium tumefaciens
6-deoxy-L-galactono-1,5-lactone + NADH
-
285798
Agrobacterium tumefaciens
?
6-deoxy-L-galactose + NAD+
i.e. L-fucose
285798
Agrobacterium tumefaciens K-28
6-deoxy-L-galactono-1,5-lactone + NADH
-
285798
Agrobacterium tumefaciens K-28
?
D-arabinose + NAD+
74.5% of the activity compared to L-fucose
285798
Agrobacterium tumefaciens
D-arabinono-1,4-lactone + NADH
-
285798
Agrobacterium tumefaciens
?
D-arabinose + NAD+
74.5% of the activity compared to L-fucose
285798
Agrobacterium tumefaciens K-28
D-arabinono-1,4-lactone + NADH
-
285798
Agrobacterium tumefaciens K-28
?
fuconolactone + NADPH + H+
-
285798
Agrobacterium tumefaciens
L-fucose + NADP+
-
-
-
-
fuconolactone + NADPH + H+
-
285798
Agrobacterium tumefaciens K-28
L-fucose + NADP+
-
-
-
-
L-galactose + NAD+
59.6% of the activity compared to L-fucose
285798
Agrobacterium tumefaciens
L-galactono-1,5-lactone + NADH
-
285798
Agrobacterium tumefaciens
?
L-galactose + NAD+
59.6% of the activity compared to L-fucose
285798
Agrobacterium tumefaciens K-28
L-galactono-1,5-lactone + NADH
-
285798
Agrobacterium tumefaciens K-28
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
50
-
Agrobacterium tumefaciens
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
40
-
stable at
Agrobacterium tumefaciens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
10
-
Agrobacterium tumefaciens
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
7.5
9
stable within
Agrobacterium tumefaciens
Other publictions for EC 1.1.1.122
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
681445
Brouns
Crystal structure and biochemi ...
Sulfolobus solfataricus, Sulfolobus solfataricus P2
J. Mol. Biol.
371
1249-1260
2007
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-
-
1
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1
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3
-
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2
1
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1
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1
1
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2
1
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-
-
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-
-
-
-
719837
Brouns
Identification of the missing ...
Sulfolobus solfataricus, Sulfolobus solfataricus P2
J. Biol. Chem.
281
27378-27388
2006
-
-
1
-
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1
2
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2
1
1
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2
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1
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1
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1
1
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1
2
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2
1
1
-
2
-
1
-
-
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-
-
-
-
-
-
655086
Bakke
-
Improvement in thermal stabili ...
Pseudomonas sp., Pseudomonas sp. No. 1143
Biotechnol. Lett.
24
1075-1078
2002
-
-
-
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-
1
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2
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2
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2
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1
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2
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2
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285798
Itoh
-
Purification and characterizat ...
Agrobacterium tumefaciens, Agrobacterium tumefaciens K-28
J. Ferment. Bioeng.
77
100-102
1994
-
-
-
-
-
-
4
5
-
-
1
6
-
2
-
-
1
-
-
1
2
-
8
-
1
-
1
-
1
-
1
1
-
-
-
-
-
-
1
-
-
-
-
4
-
5
-
-
1
6
-
-
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1
-
1
2
-
8
-
1
-
1
-
1
-
1
-
-
-
-
-
-
-
285799
Yamamoto-Otake
Cloning and sequencing of the ...
Pseudomonas sp.
Biosci. Biotechnol. Biochem.
58
2281-2282
1994
-
-
1
-
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1
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3
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1
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1
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-
285800
Tsuji
Purification and some properti ...
Agrobacterium tumefaciens
Biochim. Biophys. Acta
1117
167-173
1992
-
1
-
-
-
1
4
4
-
-
3
3
-
4
-
-
1
-
-
1
1
1
4
1
-
-
1
-
-
1
1
2
-
-
-
-
1
-
2
-
-
1
-
4
-
4
-
-
3
3
-
-
-
1
-
1
1
1
4
1
-
-
1
-
-
1
1
-
-
-
-
-
-
-
285801
Guimaraes
-
Purification and general prope ...
Aureobasidium pullulans
Arq. Biol. Tecnol. (Curitiba)
32
575-587
1989
-
-
-
-
-
-
2
4
-
-
1
3
-
1
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1
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1
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1
3
1
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1
-
1
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1
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1
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2
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4
-
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1
3
-
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1
-
1
-
1
3
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
285802
Sasajima
Oxidation of L-glucose by a ps ...
Paraburkholderia caryophylli
Biochim. Biophys. Acta
571
120-126
1979
-
-
-
-
-
1
4
4
-
-
1
7
-
1
-
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1
-
-
1
1
1
7
-
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1
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2
-
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2
-
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1
-
4
-
4
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1
7
-
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1
-
1
1
1
7
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
285805
Endo
Isolation and characterization ...
Oryctolagus cuniculus
J. Biochem.
86
1559-1565
1979
-
-
-
-
-
-
4
3
-
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1
4
-
2
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1
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2
1
1
4
-
1
-
1
-
1
-
1
1
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1
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4
-
3
-
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1
4
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1
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2
1
1
4
-
1
-
1
-
1
-
1
-
-
-
-
-
-
-
285803
Mobley
The physical properties of NAD ...
Ovis aries aries
Arch. Biochem. Biophys.
186
184-188
1978
5
-
-
-
-
1
3
1
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3
2
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2
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1
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2
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1
2
1
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2
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1
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5
-
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1
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1
-
3
-
1
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3
2
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1
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2
-
1
2
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
285804
Mobley
L-Fucose dehydrogenase from sh ...
Ovis aries aries
Methods Enzymol.
41B
173-177
1975
-
-
-
-
-
1
-
6
-
-
1
4
-
2
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1
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2
-
1
4
-
-
-
1
-
1
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1
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-
1
-
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1
-
-
-
6
-
-
1
4
-
-
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1
-
2
-
1
4
-
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-
1
-
1
-
-
-
-
-
-
-
-
-
285806
Schacter
Isolation of diphosphopyridine ...
Sus scrofa
J. Biol. Chem.
244
4785-4792
1969
1
-
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4
7
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6
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2
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1
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2
1
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6
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1
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1
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1
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1
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4
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7
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6
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1
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2
1
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6
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1
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