BRENDA - Enzyme Database show
show all sequences of 1.1.1.121

Analysis of bacterial glucose dehydrogenase homologs from thermoacidophilic archaeon Thermoplasma acidophilum: finding and characterization of aldohexose dehydrogenase

Nishiya, Y.; Tamura, N.; Tamura, T.; Biosci. Biotechnol. Biochem. 68, 2451-2456 (2004)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
-
Thermoplasma acidophilum
Inhibitors
Inhibitors
Commentary
Organism
Structure
Ag+
-
Thermoplasma acidophilum
Cu2+
-
Thermoplasma acidophilum
Hg2+
-
Thermoplasma acidophilum
Ni2+
-
Thermoplasma acidophilum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
24.3
-
2-deoxy-D-glucose
pH 8.0, 37C
Thermoplasma acidophilum
25
-
D-mannose
pH 8.0, 37C
Thermoplasma acidophilum
208
-
D-glucose
pH 8.0, 37C
Thermoplasma acidophilum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
30000
-
x * 30000, SDS-PAGE
Thermoplasma acidophilum
85000
-
gel filtration
Thermoplasma acidophilum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Thermoplasma acidophilum
Q9HK51
-
-
Purification (Commentary)
Commentary
Organism
-
Thermoplasma acidophilum
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
4.5
-
pH 8.0, 37C, substrates: D-glucose + NAD+
Thermoplasma acidophilum
15
-
pH 8.0, 37C, substrates: D-xylose + NAD+
Thermoplasma acidophilum
24
-
pH 8.0, 37C, substrates: 2-deoxy-D-glucose + NAD+
Thermoplasma acidophilum
31
-
pH 8.0, 37C, substrates: D-mannose + NAD+
Thermoplasma acidophilum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-deoxy-D-glucose + NAD+
relative activity with 2-deoxy-D-glucose and NADP+ is less than 5% compared to the activity with 2-deoxy-D-glucose and NAD+
719111
Thermoplasma acidophilum
2-deoxy-D-glucono-1,5-lactone + NADH + H+
-
-
-
?
2-deoxy-D-glucose + NADP+
relative activity with 2-deoxy-D-glucose and NADP+ is less than 5% compared to the activity with 2-deoxy-D-glucose and NAD+
719111
Thermoplasma acidophilum
2-deoxy-D-glucono-1,5-lactone + NADPH + H+
-
-
-
?
D-glucose + NAD+
activity is 23% compared to the activity with D-mannose and NAD+. No activity with beta-D-glucose + NADP+
719111
Thermoplasma acidophilum
2-deoxy-D-glucono-1,5-lactone + NADH + H+
-
-
-
?
D-mannose + NAD+
relative activity with D-mannose and NADP+ is 7% compared to the activity with D-mannose and NAD+
719111
Thermoplasma acidophilum
D-mannono-1,5-lactone + NADH
-
-
-
?
D-mannose + NADP+
relative activity with D-mannose and NADP+ is 7% compared to the activity with D-mannose and NAD+
719111
Thermoplasma acidophilum
D-mannono-1,5-lactone + NADPH
-
-
-
?
D-xylose + NAD+
relative activity with D-xylose and NADP+ is less than 5% compared to the activity with D-xylose and NAD+
719111
Thermoplasma acidophilum
D-xylono-1,5-lactone + NADH + H+
-
-
-
?
D-xylose + NADP+
relative activity with D-xylose and NADP+ is less than 5% compared to the activity with D-xylose and NAD+
719111
Thermoplasma acidophilum
D-xylono-1,5-lactone + NADPH + H+
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 30000, SDS-PAGE
Thermoplasma acidophilum
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Thermoplasma acidophilum
70
-
-
Thermoplasma acidophilum
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
60
-
stable up to, pH 7.5, 15 min
Thermoplasma acidophilum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Thermoplasma acidophilum
10
-
-
Thermoplasma acidophilum
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
7
11
25C, 16 h, no loss of activity
Thermoplasma acidophilum
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
relative activity with D-mannose and NADP+ is 7% compared to the activity with D-mannose and NAD+. Relative activity with 2-deoxy-D-glucose and NADP+ is less than 5% compared to the activity with 2-deoxy-D-glucose and NAD+
Thermoplasma acidophilum
NADP+
relative activity with D-mannose and NADP+ is 7% compared to the activity with D-mannose and NAD+. Relative activity with 2-deoxy-D-glucose and NADP+ is less than 5% compared to the activity with 2-deoxy-D-glucose and NAD+
Thermoplasma acidophilum
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Thermoplasma acidophilum
isoelectric focusing
-
5.2
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Thermoplasma acidophilum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
relative activity with D-mannose and NADP+ is 7% compared to the activity with D-mannose and NAD+. Relative activity with 2-deoxy-D-glucose and NADP+ is less than 5% compared to the activity with 2-deoxy-D-glucose and NAD+
Thermoplasma acidophilum
NADP+
relative activity with D-mannose and NADP+ is 7% compared to the activity with D-mannose and NAD+. Relative activity with 2-deoxy-D-glucose and NADP+ is less than 5% compared to the activity with 2-deoxy-D-glucose and NAD+
Thermoplasma acidophilum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ag+
-
Thermoplasma acidophilum
Cu2+
-
Thermoplasma acidophilum
Hg2+
-
Thermoplasma acidophilum
Ni2+
-
Thermoplasma acidophilum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
24.3
-
2-deoxy-D-glucose
pH 8.0, 37C
Thermoplasma acidophilum
25
-
D-mannose
pH 8.0, 37C
Thermoplasma acidophilum
208
-
D-glucose
pH 8.0, 37C
Thermoplasma acidophilum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
30000
-
x * 30000, SDS-PAGE
Thermoplasma acidophilum
85000
-
gel filtration
Thermoplasma acidophilum
Purification (Commentary) (protein specific)
Commentary
Organism
-
Thermoplasma acidophilum
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
4.5
-
pH 8.0, 37C, substrates: D-glucose + NAD+
Thermoplasma acidophilum
15
-
pH 8.0, 37C, substrates: D-xylose + NAD+
Thermoplasma acidophilum
24
-
pH 8.0, 37C, substrates: 2-deoxy-D-glucose + NAD+
Thermoplasma acidophilum
31
-
pH 8.0, 37C, substrates: D-mannose + NAD+
Thermoplasma acidophilum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-deoxy-D-glucose + NAD+
relative activity with 2-deoxy-D-glucose and NADP+ is less than 5% compared to the activity with 2-deoxy-D-glucose and NAD+
719111
Thermoplasma acidophilum
2-deoxy-D-glucono-1,5-lactone + NADH + H+
-
-
-
?
2-deoxy-D-glucose + NADP+
relative activity with 2-deoxy-D-glucose and NADP+ is less than 5% compared to the activity with 2-deoxy-D-glucose and NAD+
719111
Thermoplasma acidophilum
2-deoxy-D-glucono-1,5-lactone + NADPH + H+
-
-
-
?
D-glucose + NAD+
activity is 23% compared to the activity with D-mannose and NAD+. No activity with beta-D-glucose + NADP+
719111
Thermoplasma acidophilum
2-deoxy-D-glucono-1,5-lactone + NADH + H+
-
-
-
?
D-mannose + NAD+
relative activity with D-mannose and NADP+ is 7% compared to the activity with D-mannose and NAD+
719111
Thermoplasma acidophilum
D-mannono-1,5-lactone + NADH
-
-
-
?
D-mannose + NADP+
relative activity with D-mannose and NADP+ is 7% compared to the activity with D-mannose and NAD+
719111
Thermoplasma acidophilum
D-mannono-1,5-lactone + NADPH
-
-
-
?
D-xylose + NAD+
relative activity with D-xylose and NADP+ is less than 5% compared to the activity with D-xylose and NAD+
719111
Thermoplasma acidophilum
D-xylono-1,5-lactone + NADH + H+
-
-
-
?
D-xylose + NADP+
relative activity with D-xylose and NADP+ is less than 5% compared to the activity with D-xylose and NAD+
719111
Thermoplasma acidophilum
D-xylono-1,5-lactone + NADPH + H+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 30000, SDS-PAGE
Thermoplasma acidophilum
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Thermoplasma acidophilum
70
-
-
Thermoplasma acidophilum
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
60
-
stable up to, pH 7.5, 15 min
Thermoplasma acidophilum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Thermoplasma acidophilum
10
-
-
Thermoplasma acidophilum
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
7
11
25C, 16 h, no loss of activity
Thermoplasma acidophilum
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Thermoplasma acidophilum
isoelectric focusing
-
5.2
Other publictions for EC 1.1.1.121
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
697263
Asada
Biochemical and structural cha ...
Thermus thermophilus HB8
Chem. Biol. Interact.
178
117-126
2009
1
-
1
1
3
-
7
25
-
-
2
-
-
1
-
-
1
-
-
-
-
1
29
1
-
1
1
25
-
-
1
2
4
-
6
1
-
1
2
1
3
-
6
7
4
25
-
-
2
-
-
-
-
1
-
-
-
1
29
1
-
1
1
25
-
-
1
-
-
-
-
-
-
-
688344
Yasutake
Structural insights into uniqu ...
Thermoplasma acidophilum
J. Mol. Biol.
367
1034-1046
2007
-
-
1
1
-
-
-
-
-
-
-
1
-
1
-
-
1
1
-
-
-
-
3
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
718488
Yasutake
Crystallization and preliminar ...
Thermoplasma acidophilum
Acta Crystallogr. Sect. F
62
586-589
2006
-
-
-
1
-
-
-
-
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
719111
Nishiya
Analysis of bacterial glucose ...
Thermoplasma acidophilum
Biosci. Biotechnol. Biochem.
68
2451-2456
2004
-
-
1
-
-
-
4
3
-
-
2
-
-
4
-
-
1
-
-
-
4
-
7
1
2
-
1
-
2
-
1
2
-
1
-
-
-
1
2
-
-
-
-
4
-
3
-
-
2
-
-
-
-
1
-
-
4
-
7
1
2
-
1
-
2
-
1
1
-
-
-
-
-
-
5692
Green
Carbohydrate metabolism in som ...
Methylobacterium mesophilicum, Methylobacterium organophilum, Methylobacterium organophilum XX, Pseudomonas sp., Pseudomonas sp. NCIB10602 NCIB10603
FEMS Microbiol. Lett.
23
31-34
1984
-
-
-
-
-
-
-
-
-
-
-
2
-
6
-
-
-
-
-
-
-
-
23
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
23
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285794
Dahms
D-Fucose metabolism in a pseud ...
Pseudomonas sp., Pseudomonas sp. MSU-1
J. Biol. Chem.
247
2222-2227
1972
-
-
-
-
-
-
8
9
-
-
-
2
-
8
-
-
1
-
-
1
1
1
20
-
1
-
1
-
2
2
-
2
-
-
-
-
-
-
2
-
-
-
-
8
-
9
-
-
-
2
-
-
-
1
-
1
1
1
20
-
1
-
1
-
2
2
-
-
-
-
-
-
-
-
285790
Cline
The isolation of three sugar d ...
Pseudomonas sp., Pseudomonas sp. G6
J. Biol. Chem.
240
4488-4492
1965
-
-
-
-
-
-
-
-
-
-
-
2
-
5
-
-
1
-
-
-
-
4
4
-
1
-
1
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
4
4
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
285791
Cline
Enzymatic characterization and ...
Pseudomonas sp., Pseudomonas sp. G6
J. Biol. Chem.
240
4493-4497
1965
-
-
-
-
-
-
-
2
-
-
-
2
-
5
-
-
1
-
-
-
-
-
16
-
1
-
-
-
1
1
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
2
-
-
-
1
-
-
-
-
16
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
285797
Cline
Some physical properties of th ...
Pseudomonas sp., Pseudomonas sp. G6
J. Biol. Chem.
240
4498-4502
1965
-
-
-
-
-
-
-
1
-
-
3
-
-
5
-
-
1
-
-
-
-
-
-
1
-
-
1
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
3
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-