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show all sequences of 1.1.1.121

Crystallization and preliminary crystallographic analysis of NAD+-preferring aldohexose dehydrogenase from the thermoacidophilic archaeon Thermoplasma acidophilum

Yasutake, Y.; Nishiya, Y.; Tamura, N.; Tamura, T.; Acta Crystallogr. Sect. F 62, 586-589 (2006)

Data extracted from this reference:

Crystallization (Commentary)
Crystallization
Organism
hanging-drop vapour-diffusion technique at 20°C under several acidic conditions with polyethylene glycol and ammonium sulfate as precipitants. Optimization of the initial crystallizations conditions yields single crystals in solution containing 0.1 M sodium acetate pH 4.6, 18%(w/v) PEG 4000, 0.2 M ammonium sulfate and 15%(v/v) glycerol. An X-ray diffraction data set is collected to a resolution of 2.8 A
Thermoplasma acidophilum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Thermoplasma acidophilum
Q9HK51
-
-
Purification (Commentary)
Commentary
Organism
-
Thermoplasma acidophilum
Crystallization (Commentary) (protein specific)
Crystallization
Organism
hanging-drop vapour-diffusion technique at 20°C under several acidic conditions with polyethylene glycol and ammonium sulfate as precipitants. Optimization of the initial crystallizations conditions yields single crystals in solution containing 0.1 M sodium acetate pH 4.6, 18%(w/v) PEG 4000, 0.2 M ammonium sulfate and 15%(v/v) glycerol. An X-ray diffraction data set is collected to a resolution of 2.8 A
Thermoplasma acidophilum
Purification (Commentary) (protein specific)
Commentary
Organism
-
Thermoplasma acidophilum
Other publictions for EC 1.1.1.121
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
697263
Asada
Biochemical and structural cha ...
Thermus thermophilus HB8
Chem. Biol. Interact.
178
117-126
2009
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7
25
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2
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1
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1
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1
29
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1
1
25
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4
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6
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7
4
25
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1
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1
29
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1
25
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1
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688344
Yasutake
Structural insights into uniqu ...
Thermoplasma acidophilum
J. Mol. Biol.
367
1034-1046
2007
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718488
Yasutake
Crystallization and preliminar ...
Thermoplasma acidophilum
Acta Crystallogr. Sect. F
62
586-589
2006
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719111
Nishiya
Analysis of bacterial glucose ...
Thermoplasma acidophilum
Biosci. Biotechnol. Biochem.
68
2451-2456
2004
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1
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4
3
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5692
Green
Carbohydrate metabolism in som ...
Methylobacterium mesophilicum, Methylobacterium organophilum, Methylobacterium organophilum XX, Pseudomonas sp., Pseudomonas sp. NCIB10602 NCIB10603
FEMS Microbiol. Lett.
23
31-34
1984
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285794
Dahms
D-Fucose metabolism in a pseud ...
Pseudomonas sp., Pseudomonas sp. MSU-1
J. Biol. Chem.
247
2222-2227
1972
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8
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8
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2
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285790
Cline
The isolation of three sugar d ...
Pseudomonas sp., Pseudomonas sp. G6
J. Biol. Chem.
240
4488-4492
1965
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285791
Cline
Enzymatic characterization and ...
Pseudomonas sp., Pseudomonas sp. G6
J. Biol. Chem.
240
4493-4497
1965
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2
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2
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5
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1
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16
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16
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285797
Cline
Some physical properties of th ...
Pseudomonas sp., Pseudomonas sp. G6
J. Biol. Chem.
240
4498-4502
1965
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