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show all sequences of 1.1.1.121

Biochemical and structural characterization of a short-chain dehydrogenase/reductase of Thermus thermophilus HB8: a hyperthermostable aldose-1-dehydrogenase with broad substrate specificity

Asada, Y.; Endo, S.; Inoue, Y.; Mamiya, H.; Hara, A.; Kunishima, N.; Matsunaga, T.; Chem. Biol. Interact. 178, 117-126 (2009)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
additional information
kcat and Km values are increased by the elevation of temperature, whereas the kcat/Km values are not largely changed
Thermus thermophilus HB8
Cloned(Commentary)
Commentary
Organism
into vector pET-11a and overexpressed in Escherichia coli BL21 (DE3) cells
Thermus thermophilus HB8
Crystallization (Commentary)
Crystallization
Organism
by oil-microbatch method, at 1.65 A resolution. TAD follows an ordered sequential mechanism in which the coenzyme binds first
Thermus thermophilus HB8
Engineering
Amino acid exchange
Commentary
Organism
D242N
mutation results in a large decrease in thermostability of TAD without dissociation into dimer or monomer
Thermus thermophilus HB8
F141A
mutation results in decrease in thermostability of TAD without dissociation into dimer or monomer
Thermus thermophilus HB8
F249A
mutation results in decrease in thermostability of TAD without dissociation into dimer or monomer
Thermus thermophilus HB8
Inhibitors
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
uncompetitive inhibition with respect to NAD+ and competitive inhibition with respect to D-galactose
Thermus thermophilus HB8
2,3-benzofuran
uncompetitive inhibition with respect to NAD+ and competitive inhibition with respect to D-galactose
Thermus thermophilus HB8
catechol
-
Thermus thermophilus HB8
Hexestrol
uncompetitive inhibition with respect to NAD+ and competitive inhibition with respect to D-galactose
Thermus thermophilus HB8
indazole
-
Thermus thermophilus HB8
indole
uncompetitive inhibition with respect to NAD+ and competitive inhibition with respect to D-galactose
Thermus thermophilus HB8
additional information
EDTA (1 mM) and p-chloromercuriphenylsulfonate and p-chloromercuribenzoate (0.1 mM) have no effect on the activity
Thermus thermophilus HB8
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.012
-
isatin
-
Thermus thermophilus HB8
0.14
-
1-Acenaphthenol
-
Thermus thermophilus HB8
0.17
-
(S)-tetralol
-
Thermus thermophilus HB8
0.25
-
4-chromanol
-
Thermus thermophilus HB8
0.65
-
(S)-indanol
-
Thermus thermophilus HB8
1.3
-
(R)-indanol
-
Thermus thermophilus HB8
1.9
-
alpha-tetralone
-
Thermus thermophilus HB8
2
3
D-galactosamine
-
Thermus thermophilus HB8
2
-
phenyl-1,2-propanedione
-
Thermus thermophilus HB8
2.5
-
(R)-tetralol
-
Thermus thermophilus HB8
3.7
-
2-Cyclohexen-1-ol
-
Thermus thermophilus HB8
5.5
-
D-fucose
-
Thermus thermophilus HB8
27
-
cis-benzene dihydrodiol
-
Thermus thermophilus HB8
33
-
2-deoxy-D-galactose
-
Thermus thermophilus HB8
78
-
D-galactose
-
Thermus thermophilus HB8
131
-
D-talose
-
Thermus thermophilus HB8
139
-
D-glucosamine
-
Thermus thermophilus HB8
162
-
L-fucose
-
Thermus thermophilus HB8
178
-
6-deoxy-D-glucose
-
Thermus thermophilus HB8
250
-
cellobiose
-
Thermus thermophilus HB8
262
-
L-arabinose
-
Thermus thermophilus HB8
300
-
2-deoxy-D-glucose
-
Thermus thermophilus HB8
580
-
D-glucose
-
Thermus thermophilus HB8
781
-
lactose
-
Thermus thermophilus HB8
841
-
D-xylose
-
Thermus thermophilus HB8
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
27000
-
4 * 27000, SDS-PAGE
Thermus thermophilus HB8
100000
-
gel filtration
Thermus thermophilus HB8
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Thermus thermophilus HB8
Q5SLC4
-
-
Purification (Commentary)
Commentary
Organism
by sonication, heating at 70°C for 10 min, ion-exchange chromatography, gel filtration and ultrafiltration
Thermus thermophilus HB8
Storage Stability
Storage Stability
Organism
4°C
Thermus thermophilus HB8
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R)-indanol + NAD+
-
697263
Thermus thermophilus HB8
? + NADH + H+
-
-
-
?
(R)-tetralol + NAD+
-
697263
Thermus thermophilus HB8
? + NADH + H+
-
-
-
?
(S)-(+)-1,2,3,4-tetrahydro-1-naphthol + NAD+
-
697263
Thermus thermophilus HB8
? + NADH + H+
-
-
-
?
(S)-(+)-1-indanol + NAD+
-
697263
Thermus thermophilus HB8
? + NADH + H+
-
-
-
?
(S)-indanol + NAD+
-
697263
Thermus thermophilus HB8
? + NADH + H+
-
-
-
?
(S)-tetralol + NAD+
-
697263
Thermus thermophilus HB8
? + NADH + H+
-
-
-
?
1-acenaphthenol + NAD+
-
697263
Thermus thermophilus HB8
? + NADH + H+
-
-
-
?
2-cyclohexen-1-ol + NAD+
-
697263
Thermus thermophilus HB8
? + NADH + H+
-
-
-
?
2-deoxy-D-galactose + NAD+
-
697263
Thermus thermophilus HB8
? + NADH + H+
-
-
-
?
2-deoxy-D-glucose + NAD+
-
697263
Thermus thermophilus HB8
? + NADH + H+
-
-
-
?
4-chromanol + NAD+
-
697263
Thermus thermophilus HB8
? + NADH + H+
-
-
-
?
6-deoxy-D-glucose
-
697263
Thermus thermophilus HB8
? + NADH + H+
-
-
-
?
alpha-tetralone + NADH + H+
-
697263
Thermus thermophilus HB8
? + NAD+
-
-
-
?
cellobiose + NAD+
-
697263
Thermus thermophilus HB8
? + NADH + H+
-
-
-
?
cis-benzene dihydrodiol + NAD+
-
697263
Thermus thermophilus HB8
? + NADH + H+
-
-
-
?
D-fucose + NAD+
-
697263
Thermus thermophilus HB8
D-fucono-delta-lactone + NADH + H+
-
-
-
?
D-galactosamine + NAD+
-
697263
Thermus thermophilus HB8
? + NADH + H+
-
-
-
?
D-galactose + NAD+
-
697263
Thermus thermophilus HB8
D-galactono-gamma-lactone + NADH + H+
-
-
-
?
D-glucosamine + NAD+
-
697263
Thermus thermophilus HB8
? + NADH + H+
-
-
-
?
D-glucose + NAD+
-
697263
Thermus thermophilus HB8
D-glucono-delta-lactone + NADH + H+
-
-
-
?
D-talose + NAD+
-
697263
Thermus thermophilus HB8
? + NADH + H+
-
-
-
?
D-xylose + NAD+
-
697263
Thermus thermophilus HB8
? + NADH + H+
-
-
-
?
glucono-delta-lactone + NADH + H+
-
697263
Thermus thermophilus HB8
? + NAD+
-
-
-
?
isatin + NADH + H+
-
697263
Thermus thermophilus HB8
? + NAD+
-
-
-
?
L-arabinose + NAD+
-
697263
Thermus thermophilus HB8
? + NADH + H+
-
-
-
?
L-fucose + NAD+
-
697263
Thermus thermophilus HB8
? + NADH + H+
-
-
-
?
lactose + NAD+
-
697263
Thermus thermophilus HB8
? + NADH + H+
-
-
-
?
additional information
TAD possesses the catalytic triad Ser136, Tyr149, and Lys153. No activity with L-xylose, D-ribose and 2-deoxy-D-ribose. TAD does not oxidize ketoses (D-fructose and D-sorbose), sugar alcohols (D-sorbitol, galactitol, inositol, L-arabitol and xylitol), uronic acids (D-galacturonic acid and D-glucuronic acid), aldonic acids (L-gulonic acid and 2-keto-L-gulonic acid), aromatic alcohols (benzyl alcohol and catechol), aliphatic alcohols (1-nonanol, 1-decanol, 3-hydroxybutyric acid and 2,3-butanediol), and hydroxysteroids (androsterone, testosterone, 20alpha/beta-hydroxyprogesterone, cholic acid, cortisol and corticosterone). No significant activity ketones (4-nitroacetophenone, camphorquinone, 2,3-hexanedione and diacetyl) and aldehydes (4-nitrobenzaldehyde, pyridine-3-aldehyde, 1-nonanal and citral)
697263
Thermus thermophilus HB8
?
-
-
-
-
phenyl-1,2-propanedione + NADH + H+
-
697263
Thermus thermophilus HB8
? + NAD+
-
-
-
?
Subunits
Subunits
Commentary
Organism
tetramer
4 * 27000, SDS-PAGE
Thermus thermophilus HB8
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
80
85
-
Thermus thermophilus HB8
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
85
-
approximately 40% of the activity is inactivated by the incubation of the enzyme solution (0.3 mg/ml in 10 mM potassium phosphate, pH 7.0) for 15 min at 95°C
Thermus thermophilus HB8
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.075
-
(R)-tetralol
-
Thermus thermophilus HB8
0.1067
-
L-fucose
-
Thermus thermophilus HB8
0.167
-
(R)-indanol
-
Thermus thermophilus HB8
0.3117
-
D-galactosamine
-
Thermus thermophilus HB8
0.423
-
D-xylose
-
Thermus thermophilus HB8
0.6217
-
D-glucosamine
-
Thermus thermophilus HB8
3.017
-
D-talose
-
Thermus thermophilus HB8
3.5
-
2-Cyclohexen-1-ol
-
Thermus thermophilus HB8
3.85
-
D-glucose
-
Thermus thermophilus HB8
3.9
-
alpha-tetralone
-
Thermus thermophilus HB8
4.55
-
L-arabinose
-
Thermus thermophilus HB8
5.767
-
cellobiose
-
Thermus thermophilus HB8
6.35
-
6-deoxy-D-glucose
-
Thermus thermophilus HB8
6.93
-
2-deoxy-D-glucose
-
Thermus thermophilus HB8
7.383
-
cis-benzene dihydrodiol
-
Thermus thermophilus HB8
8.25
-
D-galactose
-
Thermus thermophilus HB8
8.73
-
D-fucose
-
Thermus thermophilus HB8
9.23
-
lactose
-
Thermus thermophilus HB8
12.8
-
2-deoxy-D-galactose
-
Thermus thermophilus HB8
16.83
-
phenyl-1,2-propanedione
-
Thermus thermophilus HB8
40.3
-
4-chromanol
-
Thermus thermophilus HB8
138.7
-
(S)-indanol
-
Thermus thermophilus HB8
161.7
-
(S)-tetralol
-
Thermus thermophilus HB8
433.3
-
1-Acenaphthenol
-
Thermus thermophilus HB8
981.7
-
isatin
-
Thermus thermophilus HB8
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
2
13
-
Thermus thermophilus HB8
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
TAD does not exhibit dehydrogenase activity with NADP+ as the coenzyme
Thermus thermophilus HB8
NAD+
-
Thermus thermophilus HB8
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.013
-
Hexestrol
-
Thermus thermophilus HB8
0.025
-
1,10-phenanthroline
-
Thermus thermophilus HB8
0.042
-
2,3-benzofuran
-
Thermus thermophilus HB8
0.48
-
indole
-
Thermus thermophilus HB8
IC50 Value
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.063
-
-
Thermus thermophilus HB8
Hexestrol
0.18
-
-
Thermus thermophilus HB8
1,10-phenanthroline
0.2
-
-
Thermus thermophilus HB8
2,3-benzofuran
0.48
-
-
Thermus thermophilus HB8
indole
1.2
-
-
Thermus thermophilus HB8
catechol
1.9
-
-
Thermus thermophilus HB8
indazole
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
additional information
kcat and Km values are increased by the elevation of temperature, whereas the kcat/Km values are not largely changed
Thermus thermophilus HB8
Cloned(Commentary) (protein specific)
Commentary
Organism
into vector pET-11a and overexpressed in Escherichia coli BL21 (DE3) cells
Thermus thermophilus HB8
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
TAD does not exhibit dehydrogenase activity with NADP+ as the coenzyme
Thermus thermophilus HB8
NAD+
-
Thermus thermophilus HB8
Crystallization (Commentary) (protein specific)
Crystallization
Organism
by oil-microbatch method, at 1.65 A resolution. TAD follows an ordered sequential mechanism in which the coenzyme binds first
Thermus thermophilus HB8
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
D242N
mutation results in a large decrease in thermostability of TAD without dissociation into dimer or monomer
Thermus thermophilus HB8
F141A
mutation results in decrease in thermostability of TAD without dissociation into dimer or monomer
Thermus thermophilus HB8
F249A
mutation results in decrease in thermostability of TAD without dissociation into dimer or monomer
Thermus thermophilus HB8
IC50 Value (protein specific)
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.063
-
-
Thermus thermophilus HB8
Hexestrol
0.18
-
-
Thermus thermophilus HB8
1,10-phenanthroline
0.2
-
-
Thermus thermophilus HB8
2,3-benzofuran
0.48
-
-
Thermus thermophilus HB8
indole
1.2
-
-
Thermus thermophilus HB8
catechol
1.9
-
-
Thermus thermophilus HB8
indazole
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
uncompetitive inhibition with respect to NAD+ and competitive inhibition with respect to D-galactose
Thermus thermophilus HB8
2,3-benzofuran
uncompetitive inhibition with respect to NAD+ and competitive inhibition with respect to D-galactose
Thermus thermophilus HB8
catechol
-
Thermus thermophilus HB8
Hexestrol
uncompetitive inhibition with respect to NAD+ and competitive inhibition with respect to D-galactose
Thermus thermophilus HB8
indazole
-
Thermus thermophilus HB8
indole
uncompetitive inhibition with respect to NAD+ and competitive inhibition with respect to D-galactose
Thermus thermophilus HB8
additional information
EDTA (1 mM) and p-chloromercuriphenylsulfonate and p-chloromercuribenzoate (0.1 mM) have no effect on the activity
Thermus thermophilus HB8
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.013
-
Hexestrol
-
Thermus thermophilus HB8
0.025
-
1,10-phenanthroline
-
Thermus thermophilus HB8
0.042
-
2,3-benzofuran
-
Thermus thermophilus HB8
0.48
-
indole
-
Thermus thermophilus HB8
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.012
-
isatin
-
Thermus thermophilus HB8
0.14
-
1-Acenaphthenol
-
Thermus thermophilus HB8
0.17
-
(S)-tetralol
-
Thermus thermophilus HB8
0.25
-
4-chromanol
-
Thermus thermophilus HB8
0.65
-
(S)-indanol
-
Thermus thermophilus HB8
1.3
-
(R)-indanol
-
Thermus thermophilus HB8
1.9
-
alpha-tetralone
-
Thermus thermophilus HB8
2
3
D-galactosamine
-
Thermus thermophilus HB8
2
-
phenyl-1,2-propanedione
-
Thermus thermophilus HB8
2.5
-
(R)-tetralol
-
Thermus thermophilus HB8
3.7
-
2-Cyclohexen-1-ol
-
Thermus thermophilus HB8
5.5
-
D-fucose
-
Thermus thermophilus HB8
27
-
cis-benzene dihydrodiol
-
Thermus thermophilus HB8
33
-
2-deoxy-D-galactose
-
Thermus thermophilus HB8
78
-
D-galactose
-
Thermus thermophilus HB8
131
-
D-talose
-
Thermus thermophilus HB8
139
-
D-glucosamine
-
Thermus thermophilus HB8
162
-
L-fucose
-
Thermus thermophilus HB8
178
-
6-deoxy-D-glucose
-
Thermus thermophilus HB8
250
-
cellobiose
-
Thermus thermophilus HB8
262
-
L-arabinose
-
Thermus thermophilus HB8
300
-
2-deoxy-D-glucose
-
Thermus thermophilus HB8
580
-
D-glucose
-
Thermus thermophilus HB8
781
-
lactose
-
Thermus thermophilus HB8
841
-
D-xylose
-
Thermus thermophilus HB8
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
27000
-
4 * 27000, SDS-PAGE
Thermus thermophilus HB8
100000
-
gel filtration
Thermus thermophilus HB8
Purification (Commentary) (protein specific)
Commentary
Organism
by sonication, heating at 70°C for 10 min, ion-exchange chromatography, gel filtration and ultrafiltration
Thermus thermophilus HB8
Storage Stability (protein specific)
Storage Stability
Organism
4°C
Thermus thermophilus HB8
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R)-indanol + NAD+
-
697263
Thermus thermophilus HB8
? + NADH + H+
-
-
-
?
(R)-tetralol + NAD+
-
697263
Thermus thermophilus HB8
? + NADH + H+
-
-
-
?
(S)-(+)-1,2,3,4-tetrahydro-1-naphthol + NAD+
-
697263
Thermus thermophilus HB8
? + NADH + H+
-
-
-
?
(S)-(+)-1-indanol + NAD+
-
697263
Thermus thermophilus HB8
? + NADH + H+
-
-
-
?
(S)-indanol + NAD+
-
697263
Thermus thermophilus HB8
? + NADH + H+
-
-
-
?
(S)-tetralol + NAD+
-
697263
Thermus thermophilus HB8
? + NADH + H+
-
-
-
?
1-acenaphthenol + NAD+
-
697263
Thermus thermophilus HB8
? + NADH + H+
-
-
-
?
2-cyclohexen-1-ol + NAD+
-
697263
Thermus thermophilus HB8
? + NADH + H+
-
-
-
?
2-deoxy-D-galactose + NAD+
-
697263
Thermus thermophilus HB8
? + NADH + H+
-
-
-
?
2-deoxy-D-glucose + NAD+
-
697263
Thermus thermophilus HB8
? + NADH + H+
-
-
-
?
4-chromanol + NAD+
-
697263
Thermus thermophilus HB8
? + NADH + H+
-
-
-
?
6-deoxy-D-glucose
-
697263
Thermus thermophilus HB8
? + NADH + H+
-
-
-
?
alpha-tetralone + NADH + H+
-
697263
Thermus thermophilus HB8
? + NAD+
-
-
-
?
cellobiose + NAD+
-
697263
Thermus thermophilus HB8
? + NADH + H+
-
-
-
?
cis-benzene dihydrodiol + NAD+
-
697263
Thermus thermophilus HB8
? + NADH + H+
-
-
-
?
D-fucose + NAD+
-
697263
Thermus thermophilus HB8
D-fucono-delta-lactone + NADH + H+
-
-
-
?
D-galactosamine + NAD+
-
697263
Thermus thermophilus HB8
? + NADH + H+
-
-
-
?
D-galactose + NAD+
-
697263
Thermus thermophilus HB8
D-galactono-gamma-lactone + NADH + H+
-
-
-
?
D-glucosamine + NAD+
-
697263
Thermus thermophilus HB8
? + NADH + H+
-
-
-
?
D-glucose + NAD+
-
697263
Thermus thermophilus HB8
D-glucono-delta-lactone + NADH + H+
-
-
-
?
D-talose + NAD+
-
697263
Thermus thermophilus HB8
? + NADH + H+
-
-
-
?
D-xylose + NAD+
-
697263
Thermus thermophilus HB8
? + NADH + H+
-
-
-
?
glucono-delta-lactone + NADH + H+
-
697263
Thermus thermophilus HB8
? + NAD+
-
-
-
?
isatin + NADH + H+
-
697263
Thermus thermophilus HB8
? + NAD+
-
-
-
?
L-arabinose + NAD+
-
697263
Thermus thermophilus HB8
? + NADH + H+
-
-
-
?
L-fucose + NAD+
-
697263
Thermus thermophilus HB8
? + NADH + H+
-
-
-
?
lactose + NAD+
-
697263
Thermus thermophilus HB8
? + NADH + H+
-
-
-
?
additional information
TAD possesses the catalytic triad Ser136, Tyr149, and Lys153. No activity with L-xylose, D-ribose and 2-deoxy-D-ribose. TAD does not oxidize ketoses (D-fructose and D-sorbose), sugar alcohols (D-sorbitol, galactitol, inositol, L-arabitol and xylitol), uronic acids (D-galacturonic acid and D-glucuronic acid), aldonic acids (L-gulonic acid and 2-keto-L-gulonic acid), aromatic alcohols (benzyl alcohol and catechol), aliphatic alcohols (1-nonanol, 1-decanol, 3-hydroxybutyric acid and 2,3-butanediol), and hydroxysteroids (androsterone, testosterone, 20alpha/beta-hydroxyprogesterone, cholic acid, cortisol and corticosterone). No significant activity ketones (4-nitroacetophenone, camphorquinone, 2,3-hexanedione and diacetyl) and aldehydes (4-nitrobenzaldehyde, pyridine-3-aldehyde, 1-nonanal and citral)
697263
Thermus thermophilus HB8
?
-
-
-
-
phenyl-1,2-propanedione + NADH + H+
-
697263
Thermus thermophilus HB8
? + NAD+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 27000, SDS-PAGE
Thermus thermophilus HB8
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
80
85
-
Thermus thermophilus HB8
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
85
-
approximately 40% of the activity is inactivated by the incubation of the enzyme solution (0.3 mg/ml in 10 mM potassium phosphate, pH 7.0) for 15 min at 95°C
Thermus thermophilus HB8
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.075
-
(R)-tetralol
-
Thermus thermophilus HB8
0.1067
-
L-fucose
-
Thermus thermophilus HB8
0.167
-
(R)-indanol
-
Thermus thermophilus HB8
0.3117
-
D-galactosamine
-
Thermus thermophilus HB8
0.423
-
D-xylose
-
Thermus thermophilus HB8
0.6217
-
D-glucosamine
-
Thermus thermophilus HB8
3.017
-
D-talose
-
Thermus thermophilus HB8
3.5
-
2-Cyclohexen-1-ol
-
Thermus thermophilus HB8
3.85
-
D-glucose
-
Thermus thermophilus HB8
3.9
-
alpha-tetralone
-
Thermus thermophilus HB8
4.55
-
L-arabinose
-
Thermus thermophilus HB8
5.767
-
cellobiose
-
Thermus thermophilus HB8
6.35
-
6-deoxy-D-glucose
-
Thermus thermophilus HB8
6.93
-
2-deoxy-D-glucose
-
Thermus thermophilus HB8
7.383
-
cis-benzene dihydrodiol
-
Thermus thermophilus HB8
8.25
-
D-galactose
-
Thermus thermophilus HB8
8.73
-
D-fucose
-
Thermus thermophilus HB8
9.23
-
lactose
-
Thermus thermophilus HB8
12.8
-
2-deoxy-D-galactose
-
Thermus thermophilus HB8
16.83
-
phenyl-1,2-propanedione
-
Thermus thermophilus HB8
40.3
-
4-chromanol
-
Thermus thermophilus HB8
138.7
-
(S)-indanol
-
Thermus thermophilus HB8
161.7
-
(S)-tetralol
-
Thermus thermophilus HB8
433.3
-
1-Acenaphthenol
-
Thermus thermophilus HB8
981.7
-
isatin
-
Thermus thermophilus HB8
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
2
13
-
Thermus thermophilus HB8
Other publictions for EC 1.1.1.121
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
697263
Asada
Biochemical and structural cha ...
Thermus thermophilus HB8
Chem. Biol. Interact.
178
117-126
2009
1
-
1
1
3
-
7
25
-
-
2
-
-
1
-
-
1
-
-
-
-
1
29
1
-
1
1
25
-
-
1
2
4
-
6
1
-
1
2
1
3
-
6
7
4
25
-
-
2
-
-
-
-
1
-
-
-
1
29
1
-
1
1
25
-
-
1
-
-
-
-
-
-
-
688344
Yasutake
Structural insights into uniqu ...
Thermoplasma acidophilum
J. Mol. Biol.
367
1034-1046
2007
-
-
1
1
-
-
-
-
-
-
-
1
-
1
-
-
1
1
-
-
-
-
3
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
718488
Yasutake
Crystallization and preliminar ...
Thermoplasma acidophilum
Acta Crystallogr. Sect. F
62
586-589
2006
-
-
-
1
-
-
-
-
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
719111
Nishiya
Analysis of bacterial glucose ...
Thermoplasma acidophilum
Biosci. Biotechnol. Biochem.
68
2451-2456
2004
-
-
1
-
-
-
4
3
-
-
2
-
-
4
-
-
1
-
-
-
4
-
7
1
2
-
1
-
2
-
1
2
-
1
-
-
-
1
2
-
-
-
-
4
-
3
-
-
2
-
-
-
-
1
-
-
4
-
7
1
2
-
1
-
2
-
1
1
-
-
-
-
-
-
5692
Green
Carbohydrate metabolism in som ...
Methylobacterium mesophilicum, Methylobacterium organophilum, Methylobacterium organophilum XX, Pseudomonas sp., Pseudomonas sp. NCIB10602 NCIB10603
FEMS Microbiol. Lett.
23
31-34
1984
-
-
-
-
-
-
-
-
-
-
-
2
-
6
-
-
-
-
-
-
-
-
23
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
23
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285794
Dahms
D-Fucose metabolism in a pseud ...
Pseudomonas sp., Pseudomonas sp. MSU-1
J. Biol. Chem.
247
2222-2227
1972
-
-
-
-
-
-
8
9
-
-
-
2
-
8
-
-
1
-
-
1
1
1
20
-
1
-
1
-
2
2
-
2
-
-
-
-
-
-
2
-
-
-
-
8
-
9
-
-
-
2
-
-
-
1
-
1
1
1
20
-
1
-
1
-
2
2
-
-
-
-
-
-
-
-
285790
Cline
The isolation of three sugar d ...
Pseudomonas sp., Pseudomonas sp. G6
J. Biol. Chem.
240
4488-4492
1965
-
-
-
-
-
-
-
-
-
-
-
2
-
5
-
-
1
-
-
-
-
4
4
-
1
-
1
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
4
4
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
285791
Cline
Enzymatic characterization and ...
Pseudomonas sp., Pseudomonas sp. G6
J. Biol. Chem.
240
4493-4497
1965
-
-
-
-
-
-
-
2
-
-
-
2
-
5
-
-
1
-
-
-
-
-
16
-
1
-
-
-
1
1
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
2
-
-
-
1
-
-
-
-
16
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
285797
Cline
Some physical properties of th ...
Pseudomonas sp., Pseudomonas sp. G6
J. Biol. Chem.
240
4498-4502
1965
-
-
-
-
-
-
-
1
-
-
3
-
-
5
-
-
1
-
-
-
-
-
-
1
-
-
1
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
3
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-