BRENDA - Enzyme Database show
show all sequences of 1.1.1.121

Enzymatic characterization and comparison of three sugar dehydrogenases from a pseudomonad

Cline, A.L.; Hu, A.S.L.; J. Biol. Chem. 240, 4493-4497 (1965)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.16
-
NAD+
-
Pseudomonas sp.
0.78
-
D-galactose
-
Pseudomonas sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-aldose + NAD+
Pseudomonas sp.
broad substrate specificity
D-aldonolactone + NADH
-
-
-
D-aldose + NAD+
Pseudomonas sp.
the enzyme is coordinately regulated with EC 1.1.1.120 and EC 1.1.1.117
D-aldonolactone + NADH
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas sp.
-
G6
-
Pseudomonas sp. G6
-
G6
-
Purification (Commentary)
Commentary
Organism
G6
Pseudomonas sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2,3,4-trideoxyaldopyranose + NAD+
-
285791
Pseudomonas sp.
? + NADH
-
-
-
-
2-deoxy-D-galactose + NAD+
-
285791
Pseudomonas sp.
? + NADH
-
-
-
-
2-deoxy-D-glucose + NAD+
-
285791
Pseudomonas sp.
? + NADH
-
-
-
-
5-hydroxypentanal + NAD+
-
285791
Pseudomonas sp.
? + NADH
-
-
-
-
chalcose + NAD+
demethylated
285791
Pseudomonas sp.
? + NADH
-
-
-
-
D-aldose + NAD+
broad substrate specificity
285791
Pseudomonas sp.
D-aldonolactone + NADH
-
-
-
-
D-aldose + NAD+
the enzyme is coordinately regulated with EC 1.1.1.120 and EC 1.1.1.117
285791
Pseudomonas sp.
D-aldonolactone + NADH
-
-
-
-
D-fucose + NAD+
-
285791
Pseudomonas sp.
D-fucono-delta-lactone + NADH
-
285791
Pseudomonas sp.
-
D-galactose + NAD+
beta-anomer preferred to alpha-anomer
285791
Pseudomonas sp.
D-galactono-gamma-lactone + NADH
-
285791
Pseudomonas sp.
-
D-glucose + NAD+
the most probable produced aldonolactones from this and the following substrates have not been isolated so far
285791
Pseudomonas sp.
D-glucono-delta-lactone + NADH + H+
-
285791
Pseudomonas sp.
-
D-glucose + NAD+
beta-anomer preferred over alpha-anomer
285791
Pseudomonas sp.
D-glucono-delta-lactone + NADH + H+
-
285791
Pseudomonas sp.
-
D-quinovose + NAD+
-
285791
Pseudomonas sp.
? + NADH
-
-
-
-
D-xylose + NAD+
-
285791
Pseudomonas sp.
? + NADH
-
-
-
-
L-arabinose + NAD+
alpha-anomer preferred to beta-anomer
285791
Pseudomonas sp.
? + NADH
-
-
-
-
additional information
broad substrate specificity
285791
Pseudomonas sp.
?
-
-
-
-
viosamine + NAD+
i.e. 4-amino-4,6-dideoxy-D-glucose
285791
Pseudomonas sp.
? + NADH
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
23
-
assay at
Pseudomonas sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.2
-
Tris/acetate buffer
Pseudomonas sp.
pH Range
pH Minimum
pH Maximum
Commentary
Organism
8
9.8
about 80% of maximal activity at pH 8.0 and 9.8 in Tris/acetate buffer
Pseudomonas sp.
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Pseudomonas sp.
NADP+
active at concentrations above 0.02 M
Pseudomonas sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Pseudomonas sp.
NADP+
active at concentrations above 0.02 M
Pseudomonas sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.16
-
NAD+
-
Pseudomonas sp.
0.78
-
D-galactose
-
Pseudomonas sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-aldose + NAD+
Pseudomonas sp.
broad substrate specificity
D-aldonolactone + NADH
-
-
-
D-aldose + NAD+
Pseudomonas sp.
the enzyme is coordinately regulated with EC 1.1.1.120 and EC 1.1.1.117
D-aldonolactone + NADH
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
G6
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2,3,4-trideoxyaldopyranose + NAD+
-
285791
Pseudomonas sp.
? + NADH
-
-
-
-
2-deoxy-D-galactose + NAD+
-
285791
Pseudomonas sp.
? + NADH
-
-
-
-
2-deoxy-D-glucose + NAD+
-
285791
Pseudomonas sp.
? + NADH
-
-
-
-
5-hydroxypentanal + NAD+
-
285791
Pseudomonas sp.
? + NADH
-
-
-
-
chalcose + NAD+
demethylated
285791
Pseudomonas sp.
? + NADH
-
-
-
-
D-aldose + NAD+
broad substrate specificity
285791
Pseudomonas sp.
D-aldonolactone + NADH
-
-
-
-
D-aldose + NAD+
the enzyme is coordinately regulated with EC 1.1.1.120 and EC 1.1.1.117
285791
Pseudomonas sp.
D-aldonolactone + NADH
-
-
-
-
D-fucose + NAD+
-
285791
Pseudomonas sp.
D-fucono-delta-lactone + NADH
-
285791
Pseudomonas sp.
-
D-galactose + NAD+
beta-anomer preferred to alpha-anomer
285791
Pseudomonas sp.
D-galactono-gamma-lactone + NADH
-
285791
Pseudomonas sp.
-
D-glucose + NAD+
the most probable produced aldonolactones from this and the following substrates have not been isolated so far
285791
Pseudomonas sp.
D-glucono-delta-lactone + NADH + H+
-
285791
Pseudomonas sp.
-
D-glucose + NAD+
beta-anomer preferred over alpha-anomer
285791
Pseudomonas sp.
D-glucono-delta-lactone + NADH + H+
-
285791
Pseudomonas sp.
-
D-quinovose + NAD+
-
285791
Pseudomonas sp.
? + NADH
-
-
-
-
D-xylose + NAD+
-
285791
Pseudomonas sp.
? + NADH
-
-
-
-
L-arabinose + NAD+
alpha-anomer preferred to beta-anomer
285791
Pseudomonas sp.
? + NADH
-
-
-
-
additional information
broad substrate specificity
285791
Pseudomonas sp.
?
-
-
-
-
viosamine + NAD+
i.e. 4-amino-4,6-dideoxy-D-glucose
285791
Pseudomonas sp.
? + NADH
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
23
-
assay at
Pseudomonas sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.2
-
Tris/acetate buffer
Pseudomonas sp.
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
8
9.8
about 80% of maximal activity at pH 8.0 and 9.8 in Tris/acetate buffer
Pseudomonas sp.
Other publictions for EC 1.1.1.121
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
697263
Asada
Biochemical and structural cha ...
Thermus thermophilus HB8
Chem. Biol. Interact.
178
117-126
2009
1
-
1
1
3
-
7
25
-
-
2
-
-
1
-
-
1
-
-
-
-
1
29
1
-
1
1
25
-
-
1
2
4
-
6
1
-
1
2
1
3
-
6
7
4
25
-
-
2
-
-
-
-
1
-
-
-
1
29
1
-
1
1
25
-
-
1
-
-
-
-
-
-
-
688344
Yasutake
Structural insights into uniqu ...
Thermoplasma acidophilum
J. Mol. Biol.
367
1034-1046
2007
-
-
1
1
-
-
-
-
-
-
-
1
-
1
-
-
1
1
-
-
-
-
3
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
718488
Yasutake
Crystallization and preliminar ...
Thermoplasma acidophilum
Acta Crystallogr. Sect. F
62
586-589
2006
-
-
-
1
-
-
-
-
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
719111
Nishiya
Analysis of bacterial glucose ...
Thermoplasma acidophilum
Biosci. Biotechnol. Biochem.
68
2451-2456
2004
-
-
1
-
-
-
4
3
-
-
2
-
-
4
-
-
1
-
-
-
4
-
7
1
2
-
1
-
2
-
1
2
-
1
-
-
-
1
2
-
-
-
-
4
-
3
-
-
2
-
-
-
-
1
-
-
4
-
7
1
2
-
1
-
2
-
1
1
-
-
-
-
-
-
5692
Green
Carbohydrate metabolism in som ...
Methylobacterium mesophilicum, Methylobacterium organophilum, Methylobacterium organophilum XX, Pseudomonas sp., Pseudomonas sp. NCIB10602 NCIB10603
FEMS Microbiol. Lett.
23
31-34
1984
-
-
-
-
-
-
-
-
-
-
-
2
-
6
-
-
-
-
-
-
-
-
23
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
23
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285794
Dahms
D-Fucose metabolism in a pseud ...
Pseudomonas sp., Pseudomonas sp. MSU-1
J. Biol. Chem.
247
2222-2227
1972
-
-
-
-
-
-
8
9
-
-
-
2
-
8
-
-
1
-
-
1
1
1
20
-
1
-
1
-
2
2
-
2
-
-
-
-
-
-
2
-
-
-
-
8
-
9
-
-
-
2
-
-
-
1
-
1
1
1
20
-
1
-
1
-
2
2
-
-
-
-
-
-
-
-
285790
Cline
The isolation of three sugar d ...
Pseudomonas sp., Pseudomonas sp. G6
J. Biol. Chem.
240
4488-4492
1965
-
-
-
-
-
-
-
-
-
-
-
2
-
5
-
-
1
-
-
-
-
4
4
-
1
-
1
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
4
4
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
285791
Cline
Enzymatic characterization and ...
Pseudomonas sp., Pseudomonas sp. G6
J. Biol. Chem.
240
4493-4497
1965
-
-
-
-
-
-
-
2
-
-
-
2
-
5
-
-
1
-
-
-
-
-
16
-
1
-
-
-
1
1
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
2
-
-
-
1
-
-
-
-
16
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
285797
Cline
Some physical properties of th ...
Pseudomonas sp., Pseudomonas sp. G6
J. Biol. Chem.
240
4498-4502
1965
-
-
-
-
-
-
-
1
-
-
3
-
-
5
-
-
1
-
-
-
-
-
-
1
-
-
1
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
3
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-