BRENDA - Enzyme Database show
show all sequences of 1.1.1.120

Anreicherung und Charakterisierung einer NADP+-abhängigen Abequosedehydrogenase aus Pseudomonas putida

Schiwara, H.W.; Domagk, G.F.; Hoppe-Seyler's Z. Physiol. Chem. 349, 1321-1329 (1968)

Data extracted from this reference:

General Stability
General Stability
Organism
very stable at -15°C
Pseudomonas putida
Inhibitors
Inhibitors
Commentary
Organism
Structure
cysteine
82% inhibition at 20 mM
Pseudomonas putida
glutathione
27% inhibition at 20 mM
Pseudomonas putida
Hg2+
complete inhibition at 1 mM
Pseudomonas putida
p-chloromercuribenzoate
complete inhibition at 1 mM, partial reversible by low concentration of cysteine
Pseudomonas putida
thioglycolate
96% inhibition at 10 mM
Pseudomonas putida
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.037
-
NADP+
-
Pseudomonas putida
3.3
-
3,6-Dideoxy-D-galactose
-
Pseudomonas putida
12
-
2-deoxy-D-galactose
-
Pseudomonas putida
48
-
3-Deoxy-D-galactose
-
Pseudomonas putida
60
-
6-deoxy-D-galactose
-
Pseudomonas putida
130
-
2,6-dideoxy-D-allose
-
Pseudomonas putida
420
-
D-galactose
-
Pseudomonas putida
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-galactose + NADP+
Pseudomonas putida
furanose form, beta-configuration
D-galactonolactone + NADPH
gamma-lactone
Pseudomonas putida
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas putida
-
-
-
Purification (Commentary)
Commentary
Organism
partial
Pseudomonas putida
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Pseudomonas putida
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.88
-
substrate: D-galactose
Pseudomonas putida
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2,6-dideoxy-D-allofuranose + NADP+
i.e. digitoxose, furanose form, 81% of the activity compared to abequose
285792
Pseudomonas putida
? + NADPH
-
285792
Pseudomonas putida
?
2-deoxy-D-galactose + NADP+
114% of the activity compared to abequose
285792
Pseudomonas putida
D-galactonolactone + NADPH
-
285792
Pseudomonas putida
?
2-deoxy-D-ribose + NADP+
18% of the activity compared to abequose
285792
Pseudomonas putida
? + NADPH
-
285792
Pseudomonas putida
?
3,6-dideoxy-beta-D-galactofuranose + NADP+
i.e. abequose, furanose form, beta-configuration
285792
Pseudomonas putida
3,6-dideoxy-D-galactono-1,4-lactone + NADPH
-
285792
Pseudomonas putida
?
3-deoxy-D-galactose + NADP+
36% of the activity compared to abequose
285792
Pseudomonas putida
D-galactonolactone + NADPH
-
285792
Pseudomonas putida
?
6-deoxy-D-galactose + NADP+
94% of the activity compared to abequose
285792
Pseudomonas putida
D-galactonolactone + NADPH
-
285792
Pseudomonas putida
?
beta-D-galactofuranose + NADP+
furanose form, beta-configuration
285792
Pseudomonas putida
D-galactono-1,4-lactone + NADPH
-
285792
Pseudomonas putida
?
D-arabinose + NADP+
5% of the activity compared to abequose
285792
Pseudomonas putida
D-arabinolactone + NADPH
-
285792
Pseudomonas putida
?
D-galactose + NADP+
furanose form, beta-configuration
285792
Pseudomonas putida
D-galactonolactone + NADPH
gamma-lactone
285792
Pseudomonas putida
?
D-ribose + NADP+
traces of activity
285792
Pseudomonas putida
? + NADPH
-
285792
Pseudomonas putida
?
L-arabinose + NADP+
6% of the compared to abequose
285792
Pseudomonas putida
L-arabinolactone + NADPH
-
285792
Pseudomonas putida
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
10.5
-
substrate: abequose
Pseudomonas putida
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
cannot be replaced by NAD+
Pseudomonas putida
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
cannot be replaced by NAD+
Pseudomonas putida
General Stability (protein specific)
General Stability
Organism
very stable at -15°C
Pseudomonas putida
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
cysteine
82% inhibition at 20 mM
Pseudomonas putida
glutathione
27% inhibition at 20 mM
Pseudomonas putida
Hg2+
complete inhibition at 1 mM
Pseudomonas putida
p-chloromercuribenzoate
complete inhibition at 1 mM, partial reversible by low concentration of cysteine
Pseudomonas putida
thioglycolate
96% inhibition at 10 mM
Pseudomonas putida
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.037
-
NADP+
-
Pseudomonas putida
3.3
-
3,6-Dideoxy-D-galactose
-
Pseudomonas putida
12
-
2-deoxy-D-galactose
-
Pseudomonas putida
48
-
3-Deoxy-D-galactose
-
Pseudomonas putida
60
-
6-deoxy-D-galactose
-
Pseudomonas putida
130
-
2,6-dideoxy-D-allose
-
Pseudomonas putida
420
-
D-galactose
-
Pseudomonas putida
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-galactose + NADP+
Pseudomonas putida
furanose form, beta-configuration
D-galactonolactone + NADPH
gamma-lactone
Pseudomonas putida
?
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Pseudomonas putida
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Pseudomonas putida
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.88
-
substrate: D-galactose
Pseudomonas putida
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2,6-dideoxy-D-allofuranose + NADP+
i.e. digitoxose, furanose form, 81% of the activity compared to abequose
285792
Pseudomonas putida
? + NADPH
-
285792
Pseudomonas putida
?
2-deoxy-D-galactose + NADP+
114% of the activity compared to abequose
285792
Pseudomonas putida
D-galactonolactone + NADPH
-
285792
Pseudomonas putida
?
2-deoxy-D-ribose + NADP+
18% of the activity compared to abequose
285792
Pseudomonas putida
? + NADPH
-
285792
Pseudomonas putida
?
3,6-dideoxy-beta-D-galactofuranose + NADP+
i.e. abequose, furanose form, beta-configuration
285792
Pseudomonas putida
3,6-dideoxy-D-galactono-1,4-lactone + NADPH
-
285792
Pseudomonas putida
?
3-deoxy-D-galactose + NADP+
36% of the activity compared to abequose
285792
Pseudomonas putida
D-galactonolactone + NADPH
-
285792
Pseudomonas putida
?
6-deoxy-D-galactose + NADP+
94% of the activity compared to abequose
285792
Pseudomonas putida
D-galactonolactone + NADPH
-
285792
Pseudomonas putida
?
beta-D-galactofuranose + NADP+
furanose form, beta-configuration
285792
Pseudomonas putida
D-galactono-1,4-lactone + NADPH
-
285792
Pseudomonas putida
?
D-arabinose + NADP+
5% of the activity compared to abequose
285792
Pseudomonas putida
D-arabinolactone + NADPH
-
285792
Pseudomonas putida
?
D-galactose + NADP+
furanose form, beta-configuration
285792
Pseudomonas putida
D-galactonolactone + NADPH
gamma-lactone
285792
Pseudomonas putida
?
D-ribose + NADP+
traces of activity
285792
Pseudomonas putida
? + NADPH
-
285792
Pseudomonas putida
?
L-arabinose + NADP+
6% of the compared to abequose
285792
Pseudomonas putida
L-arabinolactone + NADPH
-
285792
Pseudomonas putida
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
10.5
-
substrate: abequose
Pseudomonas putida
Other publictions for EC 1.1.1.120
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
285792
Schiwara
Anreicherung und Charakterisie ...
Pseudomonas putida
Hoppe-Seyler's Z. Physiol. Chem.
349
1321-1329
1968
-
-
-
-
-
1
5
7
-
-
-
1
-
1
-
-
1
-
-
1
1
-
11
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
1
-
5
-
7
-
-
-
1
-
-
-
1
-
1
1
-
11
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
285790
Cline
The isolation of three sugar d ...
Pseudomonas sp.
J. Biol. Chem.
240
4488-4492
1965
-
-
-
1
-
-
-
-
-
-
-
2
-
1
-
-
1
-
-
1
1
1
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
1
1
1
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285791
Cline
Enzymatic characterization and ...
Pseudomonas sp.
J. Biol. Chem.
240
4493-4497
1965
-
-
-
-
-
-
-
-
-
-
-
3
-
1
-
-
-
-
-
1
-
-
14
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
-
-
14
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-