BRENDA - Enzyme Database show
show all sequences of 1.1.1.120

The isolation of three sugar dehydrogenases from a pseudomonad

Cline, A.L.; Hu, A.S.L.; J. Biol. Chem. 240, 4488-4492 (1965)

Data extracted from this reference:

Crystallization (Commentary)
Crystallization
Organism
ammonium sulfate precipitation, 40% saturation
Pseudomonas sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-galactose + NADP+
Pseudomonas sp.
-
D-galactonolactone + NADPH
-
Pseudomonas sp.
?
D-galactose + NADP+
Pseudomonas sp. G6
-
D-galactonolactone + NADPH
-
Pseudomonas sp. G6
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas sp.
-
mutant strain G6
-
Purification (Commentary)
Commentary
Organism
to homogeneity, chromatography techniques
Pseudomonas sp.
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Pseudomonas sp.
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
170
-
-
Pseudomonas sp.
Storage Stability
Storage Stability
Organism
4°C, ammonium sulfate precipitate, many months, without appreciable loss of activity
Pseudomonas sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
beta-D-galactofuranose + NADP+
-
285790
Pseudomonas sp.
D-galactono-1,4-lactone + NADPH
-
285790
Pseudomonas sp.
?
beta-D-galactofuranose + NADP+
-
285790
Pseudomonas sp. G6
D-galactono-1,4-lactone + NADPH
-
285790
Pseudomonas sp. G6
?
D-galactose + NADP+
-
285790
Pseudomonas sp.
D-galactonolactone + NADPH
-
285790
Pseudomonas sp.
?
D-galactose + NADP+
-
285790
Pseudomonas sp. G6
D-galactonolactone + NADPH
-
285790
Pseudomonas sp. G6
?
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Pseudomonas sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Pseudomonas sp.
Crystallization (Commentary) (protein specific)
Crystallization
Organism
ammonium sulfate precipitation, 40% saturation
Pseudomonas sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-galactose + NADP+
Pseudomonas sp.
-
D-galactonolactone + NADPH
-
Pseudomonas sp.
?
D-galactose + NADP+
Pseudomonas sp. G6
-
D-galactonolactone + NADPH
-
Pseudomonas sp. G6
?
Purification (Commentary) (protein specific)
Commentary
Organism
to homogeneity, chromatography techniques
Pseudomonas sp.
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Pseudomonas sp.
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
170
-
-
Pseudomonas sp.
Storage Stability (protein specific)
Storage Stability
Organism
4°C, ammonium sulfate precipitate, many months, without appreciable loss of activity
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
beta-D-galactofuranose + NADP+
-
285790
Pseudomonas sp.
D-galactono-1,4-lactone + NADPH
-
285790
Pseudomonas sp.
?
beta-D-galactofuranose + NADP+
-
285790
Pseudomonas sp. G6
D-galactono-1,4-lactone + NADPH
-
285790
Pseudomonas sp. G6
?
D-galactose + NADP+
-
285790
Pseudomonas sp.
D-galactonolactone + NADPH
-
285790
Pseudomonas sp.
?
D-galactose + NADP+
-
285790
Pseudomonas sp. G6
D-galactonolactone + NADPH
-
285790
Pseudomonas sp. G6
?
Other publictions for EC 1.1.1.120
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
285792
Schiwara
Anreicherung und Charakterisie ...
Pseudomonas putida
Hoppe-Seyler's Z. Physiol. Chem.
349
1321-1329
1968
-
-
-
-
-
1
5
7
-
-
-
1
-
1
-
-
1
-
-
1
1
-
11
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
1
-
5
-
7
-
-
-
1
-
-
-
1
-
1
1
-
11
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
285790
Cline
The isolation of three sugar d ...
Pseudomonas sp.
J. Biol. Chem.
240
4488-4492
1965
-
-
-
1
-
-
-
-
-
-
-
2
-
1
-
-
1
-
-
1
1
1
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
1
1
1
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285791
Cline
Enzymatic characterization and ...
Pseudomonas sp.
J. Biol. Chem.
240
4493-4497
1965
-
-
-
-
-
-
-
-
-
-
-
3
-
1
-
-
-
-
-
1
-
-
14
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
-
-
14
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-