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Literature summary for 1.1.1.12 extracted from

  • Nikolaev, I.; Farmer Hansen, S.; Madrid, S.; de Vries, R.P.
    Disruption of the L-arabitol dehydrogenase encoding gene in Aspergillus tubingensis results in increased xylanase production (2013), Biotechnol. J., 8, 905-911.
    View publication on PubMed
Search for more literature for 1.1.1.12

Cloned(Commentary)

Cloned (Comment) Organism
gene ladA, DNA and amino acid sequence determination and analysis Aspergillus tubingensis
gene ladA, DNA and amino acid sequence determination and analysis, construction of strain 3M-43/pyrA, a pyrA mutant of strain 3M-43, in which an intact copy of ladA is inserted under control of the Aspergillus nidulans gpdA promoter. Complementation of the 3M-43 pyrA strain with pGpd:ladA Aspergillus tubingensis

Protein Variants

Protein Variants Comment Organism
additional information gene disruption of the L-arabitol dehydrogenase encoding gene resulting in increased xylanase production. Complementation with constitutively expressed ladA confirms that the xylanase overproducing phenotype is mainly caused by loss of ladA function, while a knockout of xlnR in the UV mutant demonstrates that improved xylanase production is mediated by XlnR Aspergillus tubingensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-arabinitol + NAD+ Aspergillus tubingensis
-
 L-xylulose + NADH + H+
-
 ?
L-arabinitol + NAD+ Aspergillus tubingensis 1M-7
-
 L-xylulose + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus tubingensis H6WCP4 ladA from strain 1M-7
-
Aspergillus tubingensis JQ079782 lad A from strain 3M-43
-
Aspergillus tubingensis 1M-7 H6WCP4 ladA from strain 1M-7
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information different strains are screened for the enzyme activity, the strains show differing abilities to grow on D-xylose, D-glucose, and L-arabinose, and show L-arabitol dehydrogenase LadA activity, overview Aspergillus tubingensis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arabinitol + NAD+
-
 Aspergillus tubingensis L-xylulose + NADH + H+
-
 ?
L-arabinitol + NAD+
-
 Aspergillus tubingensis 1M-7 L-xylulose + NADH + H+
-
 ?

Synonyms

Synonyms Comment Organism
L-arabitol dehydrogenase
-
 Aspergillus tubingensis
LADA
-
 Aspergillus tubingensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Aspergillus tubingensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.6
-
 assay at Aspergillus tubingensis

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Aspergillus tubingensis

General Information

General Information Comment Organism
malfunction disruption of the L-arabitol dehydrogenase encoding gene in Aspergillus tubingensis results in increased xylanase production. The xylanase overproducing phenotype is mainly caused by loss of ladA function, while improved xylanase production is mediated by XlnR Aspergillus tubingensis
malfunction disruption of the L-arabitol dehydrogenase encoding gene in Aspergillus tubingensis results in increased xylanase production. The xylanase overproducing phenotype is mainly caused by loss of ladA function, while improved xylanase production is mediated by XlnR. The ladA locus is the direct cause of the increased xylanase activity of 3M-43, a pyrA mutant of 3M-43 Aspergillus tubingensis
metabolism the enzyme is involved in the L-arabinose catabolic pathway Aspergillus tubingensis