BRENDA - Enzyme Database show
show all sequences of 1.1.1.12

Cloning, heterologous expression, and characterization of the xylitol and L-arabitol dehydrogenase genes, Texdh and Telad, from the thermophilic fungus Talaromyces emersonii

Fernandes, S.; Tuohy, M.G.; Murray, P.G.; Biochem. Genet. 48, 480-495 (2010)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Rasamsonia emersonii
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.06
-
NAD+
pH 9, 30°C
Rasamsonia emersonii
13.4
-
L-arabinitol
pH 9, 30°C
Rasamsonia emersonii
23.3
-
xylitol
pH 9, 30°C
Rasamsonia emersonii
483.5
-
D-sorbitol
pH 9, 30°C
Rasamsonia emersonii
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Zn2+
structure analysis indicates that enzyme belongs to the family of Zn2+-containing, medium-chain alcohol dehydrogenases. Residues involved in Zn2+ binding are C55, H80, and E165. Thereis a second zinc-binding site C110, C113, C116, and C124
Rasamsonia emersonii
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
41000
-
x * 41000, calculated, x * 45000, SDS-PAGE
Rasamsonia emersonii
45000
-
x * 41000, calculated, x * 45000, SDS-PAGE
Rasamsonia emersonii
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rasamsonia emersonii
C5J3R8
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-sorbitol + NAD+
-
711121
Rasamsonia emersonii
D-fructose + NADH + H+
-
-
-
?
L-arabinitol + NAD+
-
711121
Rasamsonia emersonii
L-xylulose + NADH + H+
-
-
-
?
xylitol + NAD+
-
711121
Rasamsonia emersonii
D-xylulose + NADH + H+
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 41000, calculated, x * 45000, SDS-PAGE
Rasamsonia emersonii
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
30
60
-
Rasamsonia emersonii
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.25
-
xylitol
pH 9, 30°C
Rasamsonia emersonii
3.7
-
D-sorbitol
pH 9, 30°C
Rasamsonia emersonii
4.7
-
NAD+
pH 9, 30°C
Rasamsonia emersonii
5.3
-
L-arabinitol
pH 9, 30°C
Rasamsonia emersonii
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
-
Rasamsonia emersonii
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7
11
-
Rasamsonia emersonii
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
no activity with NADP+
Rasamsonia emersonii
NAD+
specific for NAD+
Rasamsonia emersonii
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Rasamsonia emersonii
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
no activity with NADP+
Rasamsonia emersonii
NAD+
specific for NAD+
Rasamsonia emersonii
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.06
-
NAD+
pH 9, 30°C
Rasamsonia emersonii
13.4
-
L-arabinitol
pH 9, 30°C
Rasamsonia emersonii
23.3
-
xylitol
pH 9, 30°C
Rasamsonia emersonii
483.5
-
D-sorbitol
pH 9, 30°C
Rasamsonia emersonii
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Zn2+
structure analysis indicates that enzyme belongs to the family of Zn2+-containing, medium-chain alcohol dehydrogenases. Residues involved in Zn2+ binding are C55, H80, and E165. Thereis a second zinc-binding site C110, C113, C116, and C124
Rasamsonia emersonii
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
41000
-
x * 41000, calculated, x * 45000, SDS-PAGE
Rasamsonia emersonii
45000
-
x * 41000, calculated, x * 45000, SDS-PAGE
Rasamsonia emersonii
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-sorbitol + NAD+
-
711121
Rasamsonia emersonii
D-fructose + NADH + H+
-
-
-
?
L-arabinitol + NAD+
-
711121
Rasamsonia emersonii
L-xylulose + NADH + H+
-
-
-
?
xylitol + NAD+
-
711121
Rasamsonia emersonii
D-xylulose + NADH + H+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 41000, calculated, x * 45000, SDS-PAGE
Rasamsonia emersonii
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
30
60
-
Rasamsonia emersonii
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.25
-
xylitol
pH 9, 30°C
Rasamsonia emersonii
3.7
-
D-sorbitol
pH 9, 30°C
Rasamsonia emersonii
4.7
-
NAD+
pH 9, 30°C
Rasamsonia emersonii
5.3
-
L-arabinitol
pH 9, 30°C
Rasamsonia emersonii
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
-
Rasamsonia emersonii
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7
11
-
Rasamsonia emersonii
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.008
-
D-sorbitol
pH 9, 30°C
Rasamsonia emersonii
0.14
-
xylitol
pH 9, 30°C
Rasamsonia emersonii
0.39
-
L-arabinitol
pH 9, 30°C
Rasamsonia emersonii
78.3
-
NAD+
pH 9, 30°C
Rasamsonia emersonii
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.008
-
D-sorbitol
pH 9, 30°C
Rasamsonia emersonii
0.14
-
xylitol
pH 9, 30°C
Rasamsonia emersonii
0.39
-
L-arabinitol
pH 9, 30°C
Rasamsonia emersonii
78.3
-
NAD+
pH 9, 30°C
Rasamsonia emersonii
Other publictions for EC 1.1.1.12
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
710970
Kim
Cloning, characterization, and ...
Aspergillus niger, Penicillium chrysogenum, Penicillium chrysogenum Wisconsin 54-1255, Trichoderma reesei
Appl. Microbiol. Biotechnol.
87
1407-1414
2010
-
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3
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6
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4
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3
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6
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6
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3
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6
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3
-
4
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3
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6
-
-
-
-
-
-
-
-
6
6
711121
Fernandes
Cloning, heterologous expressi ...
Rasamsonia emersonii
Biochem. Genet.
48
480-495
2010
-
-
1
-
-
-
-
4
-
1
2
-
-
1
-
-
-
-
-
-
-
-
3
1
-
1
-
4
1
1
-
2
-
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1
2
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-
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-
4
-
1
2
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3
1
-
1
-
4
1
1
-
-
-
-
-
-
4
4
712767
Bae
Structure and engineering of L ...
Neurospora crassa
J. Mol. Biol.
402
230-240
2010
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1
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5
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6
-
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1
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-
1
1
4
-
-
-
-
9
-
1
-
-
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-
1
-
-
-
-
5
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6
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712797
Tiwari
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1
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Rutten
A single amino acid change (Y3 ...
Aspergillus niger
BMC Microbiol.
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Molecular regulation of arabin ...
Trichoderma reesei
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695737
Fonseca
Use of in vivo 13C nuclear mag ...
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2008
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Cloning, characterization, and ...
Neurospora crassa
Appl. Microbiol. Biotechnol.
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4
1
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4
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1
1
4
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-
-
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-
4
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1
3
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1
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2
4
1
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4
1
1
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-
-
-
686661
Fonseca
L-Arabinose transport and cata ...
Candida arabinofermentans, Candida arabinofermentans PYCC, Meyerozyma guilliermondii, Meyerozyma guilliermondii PYCC 3012
FEBS J.
274
3589-3600
2007
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4
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de Groot
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Aspergillus niger
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2005
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Aspergillus oryzae
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285789
Chiang
L-Arabinose metabolism by cell ...
Penicillium chrysogenum
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1961
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246416
Chiang
A new pathway of pentose metab ...
Penicillium chrysogenum
Biochem. Biophys. Res. Commun.
3
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1960
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