BRENDA - Enzyme Database show
show all sequences of 1.1.1.12

Cloning, characterization, and engineering of fungal L-arabinitol dehydrogenases

Kim, B.; Sullivan, R.P.; Zhao, H.; Appl. Microbiol. Biotechnol. 87, 1407-1414 (2010)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
D212S/I213R
no activity with NAD+, slight activity with NADP+
Penicillium chrysogenum
D213S/I214R
significantly higher preference for NADP+ over NAD+
Aspergillus niger
D224S/I225R
significantly altered cofactor specificity from NAD+ to NADP+
Trichoderma reesei
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.2
-
NAD+
pH 7.0, 22°C
Aspergillus niger
0.2
-
NAD+
pH 7.0, 22°C
Trichoderma reesei
0.3
-
NAD+
pH 7.0, 22°C
Penicillium chrysogenum
18
-
L-arabinitol
pH 7.0, 22°C
Trichoderma reesei
25
-
L-arabinitol
pH 7.0, 22°C
Aspergillus niger
37
-
L-arabinitol
pH 7.0, 22°C
Penicillium chrysogenum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Aspergillus niger
A2QAC0
-
-
Penicillium chrysogenum
B6HI95
strain ATCC 28089 /DSM 1075 /Wisconsin 54-1255
-
Penicillium chrysogenum Wisconsin 54-1255
B6HI95
strain ATCC 28089 /DSM 1075 /Wisconsin 54-1255
-
Trichoderma reesei
Q96V44
-
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
8.7
-
pH 7.0, 22°C
Trichoderma reesei
11.7
-
pH 7.0, 22°C
Aspergillus niger
25.3
-
pH 7.0, 22°C
Penicillium chrysogenum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-arabinitol + NAD+
-
710970
Aspergillus niger
L-xylulose + NADH + H+
-
-
-
?
L-arabinitol + NAD+
-
710970
Trichoderma reesei
L-xylulose + NADH + H+
-
-
-
?
L-arabinitol + NAD+
-
710970
Penicillium chrysogenum
L-xylulose + NADH
-
-
-
?
L-arabinitol + NAD+
-
710970
Penicillium chrysogenum Wisconsin 54-1255
L-xylulose + NADH
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
45
-
-
Aspergillus niger
45
-
-
Penicillium chrysogenum
45
-
-
Trichoderma reesei
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
5.76
-
L-arabinitol
pH 7.0, 22°C
Trichoderma reesei
7.26
-
NAD+
pH 7.0, 22°C
Trichoderma reesei
8.23
-
NAD+
pH 7.0, 22°C
Aspergillus niger
8.45
-
L-arabinitol
pH 7.0, 22°C
Aspergillus niger
17.3
-
NAD+
pH 7.0, 22°C
Penicillium chrysogenum
18.1
-
L-arabinitol
pH 7.0, 22°C
Penicillium chrysogenum
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
no activity with NADP+
Aspergillus niger
additional information
no activity with NADP+
Penicillium chrysogenum
additional information
no activity with NADP+
Trichoderma reesei
NAD+
-
Aspergillus niger
NAD+
-
Penicillium chrysogenum
NAD+
-
Trichoderma reesei
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
no activity with NADP+
Aspergillus niger
additional information
no activity with NADP+
Penicillium chrysogenum
additional information
no activity with NADP+
Trichoderma reesei
NAD+
-
Aspergillus niger
NAD+
-
Penicillium chrysogenum
NAD+
-
Trichoderma reesei
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
D212S/I213R
no activity with NAD+, slight activity with NADP+
Penicillium chrysogenum
D213S/I214R
significantly higher preference for NADP+ over NAD+
Aspergillus niger
D224S/I225R
significantly altered cofactor specificity from NAD+ to NADP+
Trichoderma reesei
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.2
-
NAD+
pH 7.0, 22°C
Aspergillus niger
0.2
-
NAD+
pH 7.0, 22°C
Trichoderma reesei
0.3
-
NAD+
pH 7.0, 22°C
Penicillium chrysogenum
18
-
L-arabinitol
pH 7.0, 22°C
Trichoderma reesei
25
-
L-arabinitol
pH 7.0, 22°C
Aspergillus niger
37
-
L-arabinitol
pH 7.0, 22°C
Penicillium chrysogenum
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
8.7
-
pH 7.0, 22°C
Trichoderma reesei
11.7
-
pH 7.0, 22°C
Aspergillus niger
25.3
-
pH 7.0, 22°C
Penicillium chrysogenum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-arabinitol + NAD+
-
710970
Aspergillus niger
L-xylulose + NADH + H+
-
-
-
?
L-arabinitol + NAD+
-
710970
Trichoderma reesei
L-xylulose + NADH + H+
-
-
-
?
L-arabinitol + NAD+
-
710970
Penicillium chrysogenum
L-xylulose + NADH
-
-
-
?
L-arabinitol + NAD+
-
710970
Penicillium chrysogenum Wisconsin 54-1255
L-xylulose + NADH
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
45
-
-
Aspergillus niger
45
-
-
Penicillium chrysogenum
45
-
-
Trichoderma reesei
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
5.76
-
L-arabinitol
pH 7.0, 22°C
Trichoderma reesei
7.26
-
NAD+
pH 7.0, 22°C
Trichoderma reesei
8.23
-
NAD+
pH 7.0, 22°C
Aspergillus niger
8.45
-
L-arabinitol
pH 7.0, 22°C
Aspergillus niger
17.3
-
NAD+
pH 7.0, 22°C
Penicillium chrysogenum
18.1
-
L-arabinitol
pH 7.0, 22°C
Penicillium chrysogenum
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.32
-
L-arabinitol
pH 7.0, 22°C
Penicillium chrysogenum
0.32
-
L-arabinitol
pH 7.0, 22°C
Trichoderma reesei
0.338
-
L-arabinitol
pH 7.0, 22°C
Aspergillus niger
41.17
-
NAD+
pH 7.0, 22°C
Aspergillus niger
44.8
-
NAD+
pH 7.0, 22°C
Trichoderma reesei
57.7
-
NAD+
pH 7.0, 22°C
Penicillium chrysogenum
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.32
-
L-arabinitol
pH 7.0, 22°C
Penicillium chrysogenum
0.32
-
L-arabinitol
pH 7.0, 22°C
Trichoderma reesei
0.338
-
L-arabinitol
pH 7.0, 22°C
Aspergillus niger
41.17
-
NAD+
pH 7.0, 22°C
Aspergillus niger
44.8
-
NAD+
pH 7.0, 22°C
Trichoderma reesei
57.7
-
NAD+
pH 7.0, 22°C
Penicillium chrysogenum
Other publictions for EC 1.1.1.12
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
710970
Kim
Cloning, characterization, and ...
Aspergillus niger, Penicillium chrysogenum, Penicillium chrysogenum Wisconsin 54-1255, Trichoderma reesei
Appl. Microbiol. Biotechnol.
87
1407-1414
2010
-
-
-
-
3
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6
-
-
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4
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3
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4
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3
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6
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6
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6
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3
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6
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3
-
4
-
3
-
-
6
-
-
-
-
-
-
-
-
6
6
711121
Fernandes
Cloning, heterologous expressi ...
Rasamsonia emersonii
Biochem. Genet.
48
480-495
2010
-
-
1
-
-
-
-
4
-
1
2
-
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1
-
-
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3
1
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1
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4
1
1
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2
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1
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4
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1
2
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3
1
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1
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4
1
1
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4
4
712767
Bae
Structure and engineering of L ...
Neurospora crassa
J. Mol. Biol.
402
230-240
2010
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1
4
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9
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1
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2
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1
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5
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6
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1
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1
1
4
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9
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1
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1
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-
-
5
-
-
-
-
6
-
-
-
-
-
-
-
-
6
6
712797
Tiwari
Molecular modeling studies of ...
Trichoderma reesei
J. Mol. Graph. Model.
28
707-713
2010
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-
-
1
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1
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4
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1
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1
1
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1
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-
-
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-
-
-
696988
Rutten
A single amino acid change (Y3 ...
Aspergillus niger
BMC Microbiol.
9
166
2009
-
-
1
1
2
-
-
10
-
-
-
-
-
3
-
-
1
-
-
-
-
-
6
-
-
-
-
12
-
-
-
1
-
-
-
-
-
1
1
1
2
-
-
-
-
10
-
-
-
-
-
-
-
1
-
-
-
-
6
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
711877
Akel
Molecular regulation of arabin ...
Trichoderma reesei
Eukaryot. Cell
8
1837-1844
2009
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1
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1
1
-
-
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695737
Fonseca
Use of in vivo 13C nuclear mag ...
Candida arabinofermentans, Candida arabinofermentans PYCC 5603T, Meyerozyma guilliermondii, Meyerozyma guilliermondii PYCC 3012
Appl. Environ. Microbiol.
74
1845-1855
2008
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5
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2
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4
-
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-
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-
684627
Sullivan
Cloning, characterization, and ...
Neurospora crassa
Appl. Microbiol. Biotechnol.
77
845-852
2007
-
1
1
-
-
-
-
4
-
1
3
-
-
3
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1
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2
4
1
1
-
2
4
1
1
-
4
-
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1
1
4
-
-
-
-
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-
4
-
1
3
-
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-
1
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-
-
2
4
1
1
-
2
4
1
1
-
-
-
-
-
-
-
-
686661
Fonseca
L-Arabinose transport and cata ...
Candida arabinofermentans, Candida arabinofermentans PYCC, Meyerozyma guilliermondii, Meyerozyma guilliermondii PYCC 3012
FEBS J.
274
3589-3600
2007
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5
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2
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4
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668063
de Groot
Metabolic control analysis of ...
Aspergillus niger
Biotechnol. Prog.
21
1610-1616
2005
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4
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1
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1
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2
2
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669541
Suzuki
Cloning and expression of NAD+ ...
Aspergillus oryzae
J. Biosci. Bioeng.
100
472-474
2005
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1
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655480
Pail
The metabolic role and evoluti ...
Trichoderma reesei
Eur. J. Biochem.
271
1864-1872
2004
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1
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14
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14
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1
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14
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1
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14
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655390
Seiboth
D-xylose metabolism in Hypocre ...
Trichoderma reesei
Eukaryot. Cell
2
867-875
2003
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285788
De Vries
Characterization of an Aspergi ...
Aspergillus nidulans
FEMS Microbiol. Lett.
123
83-90
1994
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285787
Witteveen
-
L-Arabinose and D-xylose catab ...
Aspergillus niger
J. Gen. Microbiol.
135
2163-2171
1989
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285789
Chiang
L-Arabinose metabolism by cell ...
Penicillium chrysogenum
Biochim. Biophys. Acta
46
271-278
1961
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1
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246416
Chiang
A new pathway of pentose metab ...
Penicillium chrysogenum
Biochem. Biophys. Res. Commun.
3
554-559
1960
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