BRENDA - Enzyme Database show
show all sequences of 1.1.1.12

Cloning, characterization, and mutational analysis of a highly active and stable L-arabinitol 4-dehydrogenase from Neurospora crassa

Sullivan, R.; Zhao, H.; Appl. Microbiol. Biotechnol. 77, 845-852 (2007)

Data extracted from this reference:

Application
Application
Commentary
Organism
analysis
enzymatic cycling assay for nicotinic acid adenine dinucleotide phosphate
Neurospora crassa
Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Neurospora crassa
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
18
-
L-arabinitol
pH 8.0, 25°C
Neurospora crassa
35
-
adonitol
pH 8.0, 25°C
Neurospora crassa
174
-
NAD+
pH 8.0, 25°C
Neurospora crassa
290
-
xylitol
pH 8.0, 25°C
Neurospora crassa
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Zn2+
two ions per subunit
Neurospora crassa
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
39000
-
4 * 39000, SDS-PAGE, 4 * 39600, calculated
Neurospora crassa
39600
-
4 * 39000, SDS-PAGE, 4 * 39600, calculated
Neurospora crassa
152000
-
gel filtration
Neurospora crassa
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Neurospora crassa
-
-
-
Purification (Commentary)
Commentary
Organism
recombinant protein
Neurospora crassa
Storage Stability
Storage Stability
Organism
22°C, stable for more than 1 month
Neurospora crassa
4°C, stable for several months
Neurospora crassa
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
adonitol + NAD+
-
684627
Neurospora crassa
? + NADH
-
-
-
?
L-arabinitol + NAD+
-
684627
Neurospora crassa
L-xylulose + NADH + H+
-
-
-
?
additional information
no substrate: D-mannitol, D-arabinitol, D-sorbitol
684627
Neurospora crassa
?
-
-
-
-
Xylitol + NAD+
-
684627
Neurospora crassa
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
tetramer
4 * 39000, SDS-PAGE, 4 * 39600, calculated
Neurospora crassa
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
45
55
-
Neurospora crassa
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
45
-
4 h, more than 60% residual activity
Neurospora crassa
50
-
half-life 45 min
Neurospora crassa
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
15
-
xylitol
pH 8.0, 25°C
Neurospora crassa
18
-
adonitol
pH 8.0, 25°C
Neurospora crassa
20.1
-
NAD+
pH 8.0, 25°C
Neurospora crassa
20.2
-
L-arabinitol
pH 8.0, 25°C
Neurospora crassa
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.5
-
-
Neurospora crassa
pH Range
pH Minimum
pH Maximum
Commentary
Organism
8
10.5
more than 60% of maximal activity within
Neurospora crassa
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
strong preference for NAD+
Neurospora crassa
NADH
-
Neurospora crassa
NADP+
very low yet detectable activity with NADP+, strong preference for NAD+
Neurospora crassa
NADPH
-
Neurospora crassa
Application (protein specific)
Application
Commentary
Organism
analysis
enzymatic cycling assay for nicotinic acid adenine dinucleotide phosphate
Neurospora crassa
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Neurospora crassa
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
strong preference for NAD+
Neurospora crassa
NADH
-
Neurospora crassa
NADP+
very low yet detectable activity with NADP+, strong preference for NAD+
Neurospora crassa
NADPH
-
Neurospora crassa
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
18
-
L-arabinitol
pH 8.0, 25°C
Neurospora crassa
35
-
adonitol
pH 8.0, 25°C
Neurospora crassa
174
-
NAD+
pH 8.0, 25°C
Neurospora crassa
290
-
xylitol
pH 8.0, 25°C
Neurospora crassa
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Zn2+
two ions per subunit
Neurospora crassa
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
39000
-
4 * 39000, SDS-PAGE, 4 * 39600, calculated
Neurospora crassa
39600
-
4 * 39000, SDS-PAGE, 4 * 39600, calculated
Neurospora crassa
152000
-
gel filtration
Neurospora crassa
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant protein
Neurospora crassa
Storage Stability (protein specific)
Storage Stability
Organism
22°C, stable for more than 1 month
Neurospora crassa
4°C, stable for several months
Neurospora crassa
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
adonitol + NAD+
-
684627
Neurospora crassa
? + NADH
-
-
-
?
L-arabinitol + NAD+
-
684627
Neurospora crassa
L-xylulose + NADH + H+
-
-
-
?
additional information
no substrate: D-mannitol, D-arabinitol, D-sorbitol
684627
Neurospora crassa
?
-
-
-
-
Xylitol + NAD+
-
684627
Neurospora crassa
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 39000, SDS-PAGE, 4 * 39600, calculated
Neurospora crassa
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
45
55
-
Neurospora crassa
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
45
-
4 h, more than 60% residual activity
Neurospora crassa
50
-
half-life 45 min
Neurospora crassa
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
15
-
xylitol
pH 8.0, 25°C
Neurospora crassa
18
-
adonitol
pH 8.0, 25°C
Neurospora crassa
20.1
-
NAD+
pH 8.0, 25°C
Neurospora crassa
20.2
-
L-arabinitol
pH 8.0, 25°C
Neurospora crassa
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.5
-
-
Neurospora crassa
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
8
10.5
more than 60% of maximal activity within
Neurospora crassa
Other publictions for EC 1.1.1.12
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
710970
Kim
Cloning, characterization, and ...
Aspergillus niger, Penicillium chrysogenum, Penicillium chrysogenum Wisconsin 54-1255, Trichoderma reesei
Appl. Microbiol. Biotechnol.
87
1407-1414
2010
-
-
-
-
3
-
-
6
-
-
-
-
-
4
-
-
-
-
-
-
3
-
4
-
3
-
-
6
-
-
-
6
-
-
-
-
-
-
6
-
3
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
3
-
4
-
3
-
-
6
-
-
-
-
-
-
-
-
6
6
711121
Fernandes
Cloning, heterologous expressi ...
Rasamsonia emersonii
Biochem. Genet.
48
480-495
2010
-
-
1
-
-
-
-
4
-
1
2
-
-
1
-
-
-
-
-
-
-
-
3
1
-
1
-
4
1
1
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
4
-
1
2
-
-
-
-
-
-
-
-
-
3
1
-
1
-
4
1
1
-
-
-
-
-
-
4
4
712767
Bae
Structure and engineering of L ...
Neurospora crassa
J. Mol. Biol.
402
230-240
2010
-
-
-
1
4
-
-
9
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1
-
-
-
2
-
-
1
-
-
-
-
-
5
-
-
-
-
6
-
-
-
1
-
-
-
-
-
-
1
1
4
-
-
-
-
9
-
1
-
-
-
-
-
1
-
-
-
-
5
-
-
-
-
6
-
-
-
-
-
-
-
-
6
6
712797
Tiwari
Molecular modeling studies of ...
Trichoderma reesei
J. Mol. Graph. Model.
28
707-713
2010
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
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-
-
1
1
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-
-
-
-
-
-
1
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
696988
Rutten
A single amino acid change (Y3 ...
Aspergillus niger
BMC Microbiol.
9
166
2009
-
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1
1
2
-
-
10
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-
-
-
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3
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-
1
-
-
-
-
-
6
-
-
-
-
12
-
-
-
1
-
-
-
-
-
1
1
1
2
-
-
-
-
10
-
-
-
-
-
-
-
1
-
-
-
-
6
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
711877
Akel
Molecular regulation of arabin ...
Trichoderma reesei
Eukaryot. Cell
8
1837-1844
2009
-
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-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
695737
Fonseca
Use of in vivo 13C nuclear mag ...
Candida arabinofermentans, Candida arabinofermentans PYCC 5603T, Meyerozyma guilliermondii, Meyerozyma guilliermondii PYCC 3012
Appl. Environ. Microbiol.
74
1845-1855
2008
-
-
-
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-
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5
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4
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2
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-
2
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-
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-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
684627
Sullivan
Cloning, characterization, and ...
Neurospora crassa
Appl. Microbiol. Biotechnol.
77
845-852
2007
-
1
1
-
-
-
-
4
-
1
3
-
-
3
-
-
1
-
-
-
-
2
4
1
1
-
2
4
1
1
-
4
-
-
-
-
1
1
4
-
-
-
-
-
-
4
-
1
3
-
-
-
-
1
-
-
-
2
4
1
1
-
2
4
1
1
-
-
-
-
-
-
-
-
686661
Fonseca
L-Arabinose transport and cata ...
Candida arabinofermentans, Candida arabinofermentans PYCC, Meyerozyma guilliermondii, Meyerozyma guilliermondii PYCC 3012
FEBS J.
274
3589-3600
2007
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2
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5
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2
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4
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2
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2
-
4
-
-
-
-
-
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-
-
-
668063
de Groot
Metabolic control analysis of ...
Aspergillus niger
Biotechnol. Prog.
21
1610-1616
2005
-
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-
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-
4
-
-
-
1
-
2
-
-
1
-
-
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-
2
2
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-
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2
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-
2
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4
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1
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-
1
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-
2
2
-
-
-
-
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-
-
-
-
-
-
669541
Suzuki
Cloning and expression of NAD+ ...
Aspergillus oryzae
J. Biosci. Bioeng.
100
472-474
2005
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3
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1
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1
1
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3
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Pail
The metabolic role and evoluti ...
Trichoderma reesei
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14
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1
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14
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1
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14
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1
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14
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-
-
-
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-
655390
Seiboth
D-xylose metabolism in Hypocre ...
Trichoderma reesei
Eukaryot. Cell
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2
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285788
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-
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285789
Chiang
L-Arabinose metabolism by cell ...
Penicillium chrysogenum
Biochim. Biophys. Acta
46
271-278
1961
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246416
Chiang
A new pathway of pentose metab ...
Penicillium chrysogenum
Biochem. Biophys. Res. Commun.
3
554-559
1960
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