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Literature summary for 1.1.1.119 extracted from

  • Sugii, T.; Akanuma, S.; Yagi S.; Yagyu, K.; Shimoda, Y.; Yamagishi, A.
    Characterization of the low-temperature activity of Sulfolobus tokodaii glucose-1-dehydrogenase mutants (2014), J. Biosci. Bioeng., 118, 367-371.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta2 (DE3) cells Sulfurisphaera tokodaii
expression in Escherichia coli Sulfurisphaera tokodaii

Protein Variants

Protein Variants Comment Organism
I189V the mutant has decreased kcat and kcat/Km values at both 25°C and 80°C compared to the wild type enzyme Sulfurisphaera tokodaii
V254I mutant has improved kcat and kcat/Km values at both 25°C and 80°C. The thermal stability of the mutant enzyme was comparable to that of the wild-type enzyme. Calculation of the energetic contribution of the V254I mutation for the dehydrogenase reaction reveals that the mutation destabilizes the enzyme-NADPD-glucose ternary complex and reduces the transition-state energy, thus enhancing catalysis Sulfurisphaera tokodaii
V254I the mutant has improved kcat and kcat/Km values at both 25°C and 80°C compared to the wild type enzyme Sulfurisphaera tokodaii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00588
-
NADP+ pH 9.0, 25°C, wild-type enzyme Sulfurisphaera tokodaii
0.00588
-
NADP+ wild type enzyme, at pH 9.0 and 25°C Sulfurisphaera tokodaii
0.00876
-
NADP+ pH 9.0, 25°C, mutant enzyme V254I Sulfurisphaera tokodaii
0.00876
-
NADP+ mutant enzyme V254I, at pH 9.0 and 25°C Sulfurisphaera tokodaii
0.00927
-
NADP+ pH 9.0, 25°C, mutant enzyme I189V Sulfurisphaera tokodaii
0.00927
-
NADP+ mutant enzyme I189V, at pH 9.0 and 25°C Sulfurisphaera tokodaii
0.0286
-
D-glucose pH 9.0, 25°C, wild-type enzyme Sulfurisphaera tokodaii
0.0286
-
D-glucose wild type enzyme, at pH 9.0 and 25°C Sulfurisphaera tokodaii
0.0293
-
D-glucose pH 9.0, 25°C, mutant enzyme I189V Sulfurisphaera tokodaii
0.0293
-
D-glucose mutant enzyme I189V, at pH 9.0 and 25°C Sulfurisphaera tokodaii
0.0391
-
D-glucose pH 9.0, 25°C, mutant enzyme V254I Sulfurisphaera tokodaii
0.0391
-
D-glucose mutant enzyme V254I, at pH 9.0 and 25°C Sulfurisphaera tokodaii
0.109
-
NADP+ pH 9.0, 80°C, mutant enzyme I189V Sulfurisphaera tokodaii
0.109
-
NADP+ mutant enzyme I189V, at pH 9.0 and 80°C Sulfurisphaera tokodaii
0.117
-
NADP+ pH 9.0, 80°C, wild-type enzyme Sulfurisphaera tokodaii
0.118
-
NADP+ wild type enzyme, at pH 9.0 and 80°C Sulfurisphaera tokodaii
0.132
-
NADP+ pH 9.0, 80°C, mutant enzyme V254I Sulfurisphaera tokodaii
0.132
-
NADP+ mutant enzyme V254I, at pH 9.0 and 80°C Sulfurisphaera tokodaii
0.287
-
D-glucose pH 9.0, 80°C, wild-type enzyme Sulfurisphaera tokodaii
0.287
-
D-glucose wild type enzyme, at pH 9.0 and 80°C Sulfurisphaera tokodaii
0.314
-
D-glucose pH 9.0, 80°C, mutant enzyme I189V Sulfurisphaera tokodaii
0.314
-
D-glucose mutant enzyme I189V, at pH 9.0 and 80°C Sulfurisphaera tokodaii
0.457
-
D-glucose pH 9.0, 80°C, mutant enzyme V254I Sulfurisphaera tokodaii
0.457
-
D-glucose mutant enzyme V254I, at pH 9.0 and 80°C Sulfurisphaera tokodaii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glucose + NADP+ Sulfurisphaera tokodaii
-
D-glucono-1,5-lactone + NADPH + H+
-
?
D-glucose + NADP+ Sulfurisphaera tokodaii DSM 16993
-
D-glucono-1,5-lactone + NADPH + H+
-
?

Organism

Organism UniProt Comment Textmining
Sulfurisphaera tokodaii Q96ZY1
-
-
Sulfurisphaera tokodaii Q96ZY7
-
-
Sulfurisphaera tokodaii DSM 16993 Q96ZY7
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Sulfurisphaera tokodaii
HiTrapQ column chromatography and Resource-phenyl column chromatography Sulfurisphaera tokodaii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose + NADP+
-
Sulfurisphaera tokodaii D-glucono-1,5-lactone + NADPH + H+
-
?
D-glucose + NADP+ the enzyme prefers NADP+ over NAD+ as the cofactor. It shows a larger Km value for NAD+ (4.5 mM) than for NADP+ (0.12 mM) at 80°C. At 25°C the Km values are 0.069 mM for NAD+ and 0.0059 mM for NADP+ Sulfurisphaera tokodaii D-glucono-1,5-lactone + NADPH + H+
-
?
D-glucose + NADP+
-
Sulfurisphaera tokodaii DSM 16993 D-glucono-1,5-lactone + NADPH + H+
-
?
D-glucose + NADP+ the enzyme prefers NADP+ over NAD+ as the cofactor. It shows a larger Km value for NAD+ (4.5 mM) than for NADP+ (0.12 mM) at 80°C. At 25°C the Km values are 0.069 mM for NAD+ and 0.0059 mM for NADP+ Sulfurisphaera tokodaii DSM 16993 D-glucono-1,5-lactone + NADPH + H+
-
?

Synonyms

Synonyms Comment Organism
GDH
-
Sulfurisphaera tokodaii
glucose-1-dehydrogenase
-
Sulfurisphaera tokodaii
NAD(P)-dependent GDH
-
Sulfurisphaera tokodaii
nicotinamide adenine dinucleotide (phosphate)-dependent glucose-1-dehydrogenase
-
Sulfurisphaera tokodaii
STK_17040 locus name Sulfurisphaera tokodaii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0097
-
NADP+ mutant enzyme I189V, at pH 9.0 and 80°C Sulfurisphaera tokodaii
0.0097
-
D-glucose mutant enzyme I189V, at pH 9.0 and 80°C Sulfurisphaera tokodaii
0.0101
-
NADP+ wild type enzyme, at pH 9.0 and 80°C Sulfurisphaera tokodaii
0.0101
-
D-glucose wild type enzyme, at pH 9.0 and 80°C Sulfurisphaera tokodaii
0.0167
-
NADP+ mutant enzyme V254I, at pH 9.0 and 80°C Sulfurisphaera tokodaii
0.0167
-
D-glucose mutant enzyme V254I, at pH 9.0 and 80°C Sulfurisphaera tokodaii
0.085
-
NADP+ pH 9.0, 25°C, mutant enzyme I189V Sulfurisphaera tokodaii
0.085
-
D-glucose pH 9.0, 25°C, mutant enzyme I189V Sulfurisphaera tokodaii
0.085
-
D-glucose mutant enzyme I189V, at pH 9.0 and 25°C Sulfurisphaera tokodaii
0.0857
-
NADP+ mutant enzyme I189V, at pH 9.0 and 25°C Sulfurisphaera tokodaii
0.11
-
NADP+ pH 9.0, 25°C, wild-type enzyme Sulfurisphaera tokodaii
0.11
-
D-glucose pH 9.0, 25°C, wild-type enzyme Sulfurisphaera tokodaii
0.11
-
NADP+ wild type enzyme, at pH 9.0 and 25°C Sulfurisphaera tokodaii
0.11
-
D-glucose wild type enzyme, at pH 9.0 and 25°C Sulfurisphaera tokodaii
0.205
-
NADP+ pH 9.0, 25°C, mutant enzyme V254I Sulfurisphaera tokodaii
0.205
-
D-glucose pH 9.0, 25°C, mutant enzyme V254I Sulfurisphaera tokodaii
0.205
-
NADP+ mutant enzyme V254I, at pH 9.0 and 25°C Sulfurisphaera tokodaii
0.205
-
D-glucose mutant enzyme V254I, at pH 9.0 and 25°C Sulfurisphaera tokodaii
9.7
-
NADP+ pH 9.0, 80°C, mutant enzyme I189V Sulfurisphaera tokodaii
9.7
-
D-glucose pH 9.0, 80°C, mutant enzyme I189V Sulfurisphaera tokodaii
10.1
-
NADP+ pH 9.0, 80°C, wild-type enzyme Sulfurisphaera tokodaii
10.1
-
D-glucose pH 9.0, 80°C, wild-type enzyme Sulfurisphaera tokodaii
16.7
-
NADP+ pH 9.0, 80°C, mutant enzyme V254I Sulfurisphaera tokodaii
16.7
-
D-glucose pH 9.0, 80°C, mutant enzyme V254I Sulfurisphaera tokodaii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
assay at Sulfurisphaera tokodaii

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Sulfurisphaera tokodaii
NADP+ the enzyme prefers NADP+ over NAD+ as the cofactor. It shows a larger Km value for NAD+ (4.5 mM) than for NADP+ (0.12 mM) at 80°C. At 25°C the Km values are 0.069 mM for NAD+ and 0.0059 mM for NADP+ Sulfurisphaera tokodaii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.092
-
NADP+ pH 9.0, 25°C, mutant enzyme I189V Sulfurisphaera tokodaii
0.092
-
NADP+ mutant enzyme I189V, at pH 9.0 and 25°C Sulfurisphaera tokodaii
0.126
-
NADP+ pH 9.0, 80°C, mutant enzyme V254I Sulfurisphaera tokodaii
0.126
-
NADP+ mutant enzyme V254I, at pH 9.0 and 80°C Sulfurisphaera tokodaii
0.186
-
NADP+ pH 9.0, 25°C, wild-type enzyme Sulfurisphaera tokodaii
0.186
-
NADP+ wild type enzyme, at pH 9.0 and 25°C Sulfurisphaera tokodaii
0.234
-
NADP+ pH 9.0, 25°C, mutant enzyme V254I Sulfurisphaera tokodaii
0.234
-
NADP+ mutant enzyme V254I, at pH 9.0 and 25°C Sulfurisphaera tokodaii
0.31
-
D-glucose pH 9.0, 80°C, mutant enzyme I189V Sulfurisphaera tokodaii
0.31
-
D-glucose mutant enzyme I189V, at pH 9.0 and 80°C Sulfurisphaera tokodaii
0.351
-
D-glucose pH 9.0, 80°C, wild-type enzyme Sulfurisphaera tokodaii
0.351
-
D-glucose wild type enzyme, at pH 9.0 and 80°C Sulfurisphaera tokodaii
0.365
-
D-glucose pH 9.0, 80°C, mutant enzyme V254I Sulfurisphaera tokodaii
0.365
-
D-glucose mutant enzyme V254I, at pH 9.0 and 80°C Sulfurisphaera tokodaii
0.863
-
NADP+ pH 9.0, 80°C, wild-type enzyme Sulfurisphaera tokodaii
0.863
-
NADP+ wild type enzyme, at pH 9.0 and 80°C Sulfurisphaera tokodaii
0.893
-
NADP+ pH 9.0, 80°C, mutant enzyme I189V Sulfurisphaera tokodaii
0.893
-
NADP+ mutant enzyme I189V, at pH 9.0 and 80°C Sulfurisphaera tokodaii
2.91
-
D-glucose pH 9.0, 25°C, mutant enzyme I189V Sulfurisphaera tokodaii
2.91
-
D-glucose mutant enzyme I189V, at pH 9.0 and 25°C Sulfurisphaera tokodaii
3.82
-
D-glucose pH 9.0, 25°C, wild-type enzyme Sulfurisphaera tokodaii
3.82
-
D-glucose wild type enzyme, at pH 9.0 and 25°C Sulfurisphaera tokodaii
5.25
-
D-glucose pH 9.0, 25°C, mutant enzyme V254I Sulfurisphaera tokodaii
5.25
-
D-glucose mutant enzyme V254I, at pH 9.0 and 25°C Sulfurisphaera tokodaii