Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NaCl | triple mutant D172K/D216K/D344K shows its maximum activity in a buffer with 0.50-0.75 M NaCl while the wild type protein has its maximum activity with 1.5 M NaCl | Haloferax mediterranei |
Protein Variants | Comment | Organism |
---|---|---|
D172K | mutation in surface residue, mutant protein is slightly less halotolerant than wild-type | Haloferax mediterranei |
D172K/D216K/D344K | mutation in surface residue, mutant protein is slightly less halotolerant than wild-type | Haloferax mediterranei |
D216K | mutation in surface residue, mutant protein is slightly less halotolerant than wild-type | Haloferax mediterranei |
D344K | mutation in surface residue, mutant protein is slightly less halotolerant than wild-type | Haloferax mediterranei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
KCl | at 40°C, half-life in 0.5 M KCl is 86 h for wild-type, and 114 h for mutant D172K/D216K/D344K, 95 h for mutant D172K, 117 h for mutant D216K, 114 h for mutant D344K. At 25°C, half-life in 0.5 M KCl is 506 h for wild-type, and 613 h for mutant D172K/D216K/D344K, 630 h for mutant D172K, 660 h for mutant D216K, 537 h for mutant D344K | Haloferax mediterranei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.035 | - |
NADP+ | wild-type | Haloferax mediterranei | |
0.035 | - |
NADP+ | mutant D216K | Haloferax mediterranei | |
0.036 | - |
NADP+ | mutant D172K | Haloferax mediterranei | |
0.046 | - |
NADP+ | mutant D172K/D216K/D344K | Haloferax mediterranei | |
0.056 | - |
NADP+ | mutant D344K | Haloferax mediterranei | |
1.8 | - |
D-glucose | mutant D344K | Haloferax mediterranei | |
2.2 | - |
D-glucose | mutant D216K | Haloferax mediterranei | |
2.4 | - |
D-glucose | mutant D172K | Haloferax mediterranei | |
2.8 | - |
D-glucose | wild-type | Haloferax mediterranei | |
3.2 | - |
D-glucose | mutant D172K/D216K/D344K | Haloferax mediterranei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haloferax mediterranei | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + NADP+ | - |
Haloferax mediterranei | D-glucono-1,5-lactone + NADPH + H+ | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00041 | - |
D-glucose | mutant D216K | Haloferax mediterranei | |
0.00041 | - |
D-glucose | mutant D344K | Haloferax mediterranei | |
0.00045 | - |
D-glucose | mutant D172K | Haloferax mediterranei | |
0.00045 | - |
D-glucose | mutant D172K/D216K/D344K | Haloferax mediterranei | |
0.00051 | - |
D-glucose | wild-type | Haloferax mediterranei |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Haloferax mediterranei | |
NADPH | - |
Haloferax mediterranei |