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Literature summary for 1.1.1.119 extracted from

  • Esclapez, J.; Pire, C.; Bautista, V.; Martinez-Espinosa, R.M.; Ferrer, J.; Bonete, M.J.
    Analysis of acidic surface of Haloferax mediterranei glucose dehydrogenase by site-directed mutagenesis (2007), FEBS Lett., 581, 837-842.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
NaCl triple mutant D172K/D216K/D344K shows its maximum activity in a buffer with 0.50-0.75 M NaCl while the wild type protein has its maximum activity with 1.5 M NaCl Haloferax mediterranei

Protein Variants

Protein Variants Comment Organism
D172K mutation in surface residue, mutant protein is slightly less halotolerant than wild-type Haloferax mediterranei
D172K/D216K/D344K mutation in surface residue, mutant protein is slightly less halotolerant than wild-type Haloferax mediterranei
D216K mutation in surface residue, mutant protein is slightly less halotolerant than wild-type Haloferax mediterranei
D344K mutation in surface residue, mutant protein is slightly less halotolerant than wild-type Haloferax mediterranei

Inhibitors

Inhibitors Comment Organism Structure
KCl at 40°C, half-life in 0.5 M KCl is 86 h for wild-type, and 114 h for mutant D172K/D216K/D344K, 95 h for mutant D172K, 117 h for mutant D216K, 114 h for mutant D344K. At 25°C, half-life in 0.5 M KCl is 506 h for wild-type, and 613 h for mutant D172K/D216K/D344K, 630 h for mutant D172K, 660 h for mutant D216K, 537 h for mutant D344K Haloferax mediterranei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.035
-
NADP+ wild-type Haloferax mediterranei
0.035
-
NADP+ mutant D216K Haloferax mediterranei
0.036
-
NADP+ mutant D172K Haloferax mediterranei
0.046
-
NADP+ mutant D172K/D216K/D344K Haloferax mediterranei
0.056
-
NADP+ mutant D344K Haloferax mediterranei
1.8
-
D-glucose mutant D344K Haloferax mediterranei
2.2
-
D-glucose mutant D216K Haloferax mediterranei
2.4
-
D-glucose mutant D172K Haloferax mediterranei
2.8
-
D-glucose wild-type Haloferax mediterranei
3.2
-
D-glucose mutant D172K/D216K/D344K Haloferax mediterranei

Organism

Organism UniProt Comment Textmining
Haloferax mediterranei
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose + NADP+
-
Haloferax mediterranei D-glucono-1,5-lactone + NADPH + H+
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00041
-
D-glucose mutant D216K Haloferax mediterranei
0.00041
-
D-glucose mutant D344K Haloferax mediterranei
0.00045
-
D-glucose mutant D172K Haloferax mediterranei
0.00045
-
D-glucose mutant D172K/D216K/D344K Haloferax mediterranei
0.00051
-
D-glucose wild-type Haloferax mediterranei

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Haloferax mediterranei
NADPH
-
Haloferax mediterranei