BRENDA - Enzyme Database show
show all sequences of 1.1.1.119

Aldohexose dehydrogenase from Gluconobacter cerinus-1

Avigad, G.; Englard, S.; Methods Enzymol. 41, 142-147 (1975)

Data extracted from this reference:

General Stability
General Stability
Organism
freezing and thawing: significant loss of activity
Gluconobacter cerinus
lyophilization of dilute enzyme solutions, below 0.5 ng/ml, results in significant loss of activity
Gluconobacter cerinus
Inhibitors
Inhibitors
Commentary
Organism
Structure
CuSO4
-
Gluconobacter cerinus
additional information
not: iodoacetate, N-ethylmaleimide, p-hydroxymercuribenzoate, 5,5'-dithiobis(2-nitrobenzate)
Gluconobacter cerinus
ZnSO4
-
Gluconobacter cerinus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.8
-
D-mannose
in 0.1 M Tris-buffer pH 8.0
Gluconobacter cerinus
5.3
-
D-glucose
-
Gluconobacter cerinus
5.6
-
D-mannose
in 0.1 M potassium phosphate pH 6.2
Gluconobacter cerinus
29
-
2-amino-2-deoxy-D-mannose
-
Gluconobacter cerinus
39
-
2-deoxy-D-glucose
-
Gluconobacter cerinus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Gluconobacter cerinus
-
-
-
Purification (Commentary)
Commentary
Organism
partial
Gluconobacter cerinus
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
10.1
-
-
Gluconobacter cerinus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-amino-2-deoxy-D-mannose + NADP+
-
285781
Gluconobacter cerinus
2-amino-2-deoxy-D-mannono-1,5-lactone + NADPH
-
-
-
-
2-deoxy-D-glucose + NADP+
-
285781
Gluconobacter cerinus
2-deoxy-D-glucono-1,5-lactone + NADPH + H+
-
-
-
-
D-glucose + NADP+
-
285781
Gluconobacter cerinus
D-glucono-1,5-lactone + NADPH + H+
-
-
-
-
D-mannose + NADP+
-
285781
Gluconobacter cerinus
D-mannono-1,5-lactone + NADPH
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Gluconobacter cerinus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Gluconobacter cerinus
General Stability (protein specific)
General Stability
Organism
freezing and thawing: significant loss of activity
Gluconobacter cerinus
lyophilization of dilute enzyme solutions, below 0.5 ng/ml, results in significant loss of activity
Gluconobacter cerinus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
CuSO4
-
Gluconobacter cerinus
additional information
not: iodoacetate, N-ethylmaleimide, p-hydroxymercuribenzoate, 5,5'-dithiobis(2-nitrobenzate)
Gluconobacter cerinus
ZnSO4
-
Gluconobacter cerinus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.8
-
D-mannose
in 0.1 M Tris-buffer pH 8.0
Gluconobacter cerinus
5.3
-
D-glucose
-
Gluconobacter cerinus
5.6
-
D-mannose
in 0.1 M potassium phosphate pH 6.2
Gluconobacter cerinus
29
-
2-amino-2-deoxy-D-mannose
-
Gluconobacter cerinus
39
-
2-deoxy-D-glucose
-
Gluconobacter cerinus
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Gluconobacter cerinus
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
10.1
-
-
Gluconobacter cerinus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-amino-2-deoxy-D-mannose + NADP+
-
285781
Gluconobacter cerinus
2-amino-2-deoxy-D-mannono-1,5-lactone + NADPH
-
-
-
-
2-deoxy-D-glucose + NADP+
-
285781
Gluconobacter cerinus
2-deoxy-D-glucono-1,5-lactone + NADPH + H+
-
-
-
-
D-glucose + NADP+
-
285781
Gluconobacter cerinus
D-glucono-1,5-lactone + NADPH + H+
-
-
-
-
D-mannose + NADP+
-
285781
Gluconobacter cerinus
D-mannono-1,5-lactone + NADPH
-
-
-
?
Other publictions for EC 1.1.1.119
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
728011
Sugii
Characterization of the low-te ...
Sulfolobus tokodaii, Sulfolobus tokodaii DSM 16993
J. Biosci. Bioeng.
118
367-371
2014
-
-
1
-
1
-
-
12
-
-
-
2
-
2
-
-
1
-
-
-
-
-
4
-
-
-
-
12
1
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
12
-
-
-
2
-
-
-
1
-
-
-
-
4
-
-
-
-
12
1
-
-
-
-
-
-
-
12
12
699602
Pire
-
Alteration of coenzyme specifi ...
Haloferax mediterranei
J. Mol. Catal. B
59
261-265
2009
-
-
1
-
4
-
3
25
-
-
-
-
-
2
-
-
1
-
1
-
-
1
6
-
-
-
-
7
-
-
-
2
10
-
-
-
-
2
3
-
8
-
-
3
10
34
-
-
-
-
-
-
-
1
1
-
-
1
6
-
-
-
-
7
-
-
-
-
-
-
-
-
16
16
701014
Baker
Active site dynamics in the zi ...
Haloferax mediterranei
Proc. Natl. Acad. Sci. USA
106
779-784
2009
-
-
-
1
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
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1
-
-
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-
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-
-
1
-
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-
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1
2
-
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-
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2
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
684637
Schewe
Improvement of P450(BM-3) whol ...
Bacillus megaterium
Appl. Microbiol. Biotechnol.
78
55-65
2008
-
2
1
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
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-
-
-
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2
1
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-
1
-
-
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1
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-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
696604
Sovic
The utilization of bathocuproi ...
Pseudomonas fluorescens
Bioorg. Chem.
36
91-95
2008
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
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-
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-
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1
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1
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-
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-
686754
Esclapez
Analysis of acidic surface of ...
Haloferax mediterranei
FEBS Lett.
581
837-842
2007
1
-
-
-
4
-
1
9
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
3
-
-
-
2
-
-
-
1
-
-
2
-
4
-
-
1
-
9
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
728653
Britton
Analysis of protein solvent in ...
Haloferax mediterranei, Haloferax mediterranei DSM 1411
Proc. Natl. Acad. Sci. USA
103
4846-4851
2006
-
-
-
1
-
-
-
-
-
1
-
-
-
5
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
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1
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-
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-
1
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-
-
-
-
-
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-
-
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-
-
-
-
667073
Esclapez
Crystallization and preliminar ...
Haloferax mediterranei, Haloferax mediterranei DSM 1411
Acta Crystallogr. Sect. F
61
743-746
2005
1
-
-
1
1
-
-
-
-
-
2
-
-
6
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
1
-
-
1
2
1
-
-
-
-
-
-
-
2
-
-
-
-
2
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
656318
Satoh
Enzyme-catalyzed poly(3-hydrox ...
Cupriavidus necator
J. Biosci. Bioeng.
95
335-341
2003
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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1
-
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-
-
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-
-
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-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285785
Johnsen
Different glycolytic pathways ...
Halococcus saccharolyticus
Arch. Microbiol.
175
52-61
2001
-
-
-
-
-
-
-
3
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
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-
-
3
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
726569
Ferrer
Crystallization and preliminar ...
Haloferax mediterranei, Haloferax mediterranei DSM 1411
Acta Crystallogr. Sect. D
57
1887-1889
2001
-
-
1
1
-
-
-
-
-
-
-
-
-
5
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285784
Kataoka
Escherichia coli transformant ...
Bacillus megaterium
Biosci. Biotechnol. Biochem.
62
167-169
1998
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
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1
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-
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-
-
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-
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-
-
-
-
-
-
-
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-
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-
-
-
-
-
-
-
-
-
639098
Bonete
Glucose dehydrogenase from the ...
Haloferax mediterranei, Haloferax mediterranei DSM 1411
FEBS Lett.
383
227-229
1996
-
-
-
-
-
-
1
3
-
5
2
-
-
9
-
-
1
-
-
-
1
1
4
1
-
-
1
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
1
-
3
-
5
2
-
-
-
-
1
-
-
1
1
4
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
285783
Buckmann
-
A different acceptance of aden ...
Bacillus megaterium, Filobasidium uniguttulatum
Biotechnol. Appl. Biochem.
14
104-113
1991
-
-
-
-
-
-
-
5
-
-
-
-
-
2
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
5
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
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-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
285782
Adachi
-
Crystallization and characteri ...
Gluconobacter oxydans
Agric. Biol. Chem.
44
301-308
1980
-
-
-
1
-
-
5
2
-
-
2
-
-
1
-
-
1
-
-
-
1
1
2
1
1
-
-
-
1
-
-
2
-
-
-
-
-
-
2
1
-
-
-
5
-
2
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-
2
-
-
-
-
1
-
-
1
1
2
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
285781
Avigad
Aldohexose dehydrogenase from ...
Gluconobacter cerinus
Methods Enzymol.
41
142-147
1975
-
-
-
-
-
2
3
5
-
-
-
-
-
1
-
-
1
-
-
-
1
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
2
-
3
-
5
-
-
-
-
-
-
-
1
-
-
1
-
4
-
-
-
-
-
-
-
-
-
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-
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-
285780
Avigad
Purification and properties of ...
Gluconobacter cerinus
J. Biol. Chem.
243
1936-1941
1968
-
-
-
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2
4
7
-
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-
1
-
-
1
-
-
-
1
2
4
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
2
-
-
2
-
4
-
7
-
-
-
-
-
-
-
1
-
-
1
2
4
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-