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show all sequences of 1.1.1.118

Thermostable variants constructed via the structure-guided consensus method also show increased stability in salts solutions and homogeneous aqueous-organic media

Vazquez-Figueroa, E.; Yeh, V.; Broering, J.; Chaparro-Riggers, J.; Bommarius, A.; Protein Eng. Des. Sel. 21, 673-680 (2008)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
His-tag GDH variants, into pET28a and expressed in Escherichia coli BL21(DE3)
Bacillus subtilis
Engineering
Amino acid exchange
Commentary
Organism
E170K
has enhanced stability when compared to the wild-type with half-life of ca. 9 min at 65°C. Stability dependence on salt concentration
Bacillus subtilis
E170K/Q252L
has enhanced stability when compared to the wild-type with half-life of ca. 5000 min at 65°C. The mutant demonstrates high stability regardless of salt-type at both water activities of 0.99 and 0.95
Bacillus subtilis
P45A/F155Y/V227A
has enhanced stability when compared to the wild-type with half-life of ca. 0.05 min at 65°C. Most labile variant has significantly lower stability in ethanol, acetone, and 1,4-dioxane than other variants
Bacillus subtilis
P45A/N46E/F155Y/E170K/V227A/W230F/Q252L
has enhanced stability when compared to the wild-type with half-life of ca. 5000 min at 65°C. The mutant demonstrates high stability regardless of salt-type at both water activities of 0.99 and 0.95
Bacillus subtilis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bacillus subtilis
-
strain 168
-
Bacillus subtilis 168
-
strain 168
-
Purification (Commentary)
Commentary
Organism
His-tag GDH variants
Bacillus subtilis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-glucose + NADP+
-
701045
Bacillus subtilis
D-glucono-1,5-lactone + NADPH + H+
-
-
-
?
D-glucose + NADP+
-
701045
Bacillus subtilis 168
D-glucono-1,5-lactone + NADPH + H+
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
His-tag GDH variants, into pET28a and expressed in Escherichia coli BL21(DE3)
Bacillus subtilis
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
E170K
has enhanced stability when compared to the wild-type with half-life of ca. 9 min at 65°C. Stability dependence on salt concentration
Bacillus subtilis
E170K/Q252L
has enhanced stability when compared to the wild-type with half-life of ca. 5000 min at 65°C. The mutant demonstrates high stability regardless of salt-type at both water activities of 0.99 and 0.95
Bacillus subtilis
P45A/F155Y/V227A
has enhanced stability when compared to the wild-type with half-life of ca. 0.05 min at 65°C. Most labile variant has significantly lower stability in ethanol, acetone, and 1,4-dioxane than other variants
Bacillus subtilis
P45A/N46E/F155Y/E170K/V227A/W230F/Q252L
has enhanced stability when compared to the wild-type with half-life of ca. 5000 min at 65°C. The mutant demonstrates high stability regardless of salt-type at both water activities of 0.99 and 0.95
Bacillus subtilis
Purification (Commentary) (protein specific)
Commentary
Organism
His-tag GDH variants
Bacillus subtilis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-glucose + NADP+
-
701045
Bacillus subtilis
D-glucono-1,5-lactone + NADPH + H+
-
-
-
?
D-glucose + NADP+
-
701045
Bacillus subtilis 168
D-glucono-1,5-lactone + NADPH + H+
-
-
-
?
Other publictions for EC 1.1.1.118
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739713
Zhang
Efficient 9alpha-hydroxy-4-and ...
Bacillus subtilis 168, Bacillus subtilis
SpringerPlus
5
1207
2016
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2
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72
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1
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2
1
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701045
Vazquez-Figueroa
Thermostable variants construc ...
Bacillus subtilis 168, Bacillus subtilis
Protein Eng. Des. Sel.
21
673-680
2008
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1
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4
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72
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4
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2
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668049
Manjon
-
Increased activity of glucose ...
Komagataeibacter xylinus
Biotechnol. Lett.
24
1227-1232
2002
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1
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1
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1
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1
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1
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1
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1
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1
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1
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1
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1
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1
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285779
Lin
Continuous production of L-car ...
Pseudomonas putida, Pseudomonas putida IAM 12014
J. Biosci. Bioeng.
87
361-364
1999
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1
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3
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2
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639110
Giardina
Glucose dehydrogenase from the ...
Sulfolobus solfataricus, Sulfolobus solfataricus MT-4
Biochem. J.
239
517-522
1986
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-
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8
7
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4
3
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6
20
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1
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2
3
25
2
1
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1
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1
2
1
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2
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2
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2
8
1
7
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4
3
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6
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1
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2
3
25
2
1
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1
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1
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285777
Kobayashi
-
Identification and growth char ...
Corynebacterium sp., Corynebacterium sp. No. 93-1
Agric. Biol. Chem.
44
41-47
1980
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2
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1
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1
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1
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285778
Anderson
D-Aldohexose dehydrogenase ...
Pseudomonas sp., Pseudomonas sp. MSU-1
Methods Enzymol.
41
147-150
1975
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3
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8
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1
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1
1
16
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2
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1
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1
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3
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1
1
16
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2
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285776
Hu
The regulation of some sugar d ...
Pseudomonas sp.
Biochim. Biophys. Acta
93
237-245
1964
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