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Literature summary for 1.1.1.118 extracted from

  • Vazquez-Figueroa, E.; Yeh, V.; Broering, J.; Chaparro-Riggers, J.; Bommarius, A.
    Thermostable variants constructed via the structure-guided consensus method also show increased stability in salts solutions and homogeneous aqueous-organic media (2008), Protein Eng. Des. Sel., 21, 673-680.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
His-tag GDH variants, into pET28a and expressed in Escherichia coli BL21(DE3) Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
E170K has enhanced stability when compared to the wild-type with half-life of ca. 9 min at 65°C. Stability dependence on salt concentration Bacillus subtilis
E170K/Q252L has enhanced stability when compared to the wild-type with half-life of ca. 5000 min at 65°C. The mutant demonstrates high stability regardless of salt-type at both water activities of 0.99 and 0.95 Bacillus subtilis
P45A/F155Y/V227A has enhanced stability when compared to the wild-type with half-life of ca. 0.05 min at 65°C. Most labile variant has significantly lower stability in ethanol, acetone, and 1,4-dioxane than other variants Bacillus subtilis
P45A/N46E/F155Y/E170K/V227A/W230F/Q252L has enhanced stability when compared to the wild-type with half-life of ca. 5000 min at 65°C. The mutant demonstrates high stability regardless of salt-type at both water activities of 0.99 and 0.95 Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Bacillus subtilis 168
-
-
-

Purification (Commentary)

Purification (Comment) Organism
His-tag GDH variants Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose + NADP+
-
Bacillus subtilis D-glucono-1,5-lactone + NADPH + H+
-
?
D-glucose + NADP+
-
Bacillus subtilis 168 D-glucono-1,5-lactone + NADPH + H+
-
?

Synonyms

Synonyms Comment Organism
GDH
-
Bacillus subtilis