BRENDA - Enzyme Database show
show all sequences of 1.1.1.118

Glucose dehydrogenase from the thermoacidophilic archaebacterium Sulfolobus solfataricus

Giardina, P.; DeBasia, M.G.; DeRosa, M.; Gambacort, A.; Buonocore, V.; Biochem. J. 239, 517-522 (1986)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
2-mercaptoethanol
rapid inactivation at 1 mM
Sulfolobus solfataricus
5,5'-dithiobis-(2-nitrobenzoic acid)
-
Sulfolobus solfataricus
EDTA
5% residual activity at 50 mM, addition of Mg2+ in excess restored the initial activity
Sulfolobus solfataricus
Guanidinium chloride
rapid inactivation at 4 M
Sulfolobus solfataricus
additional information
D-alactose, D-mannose and D-ribose, in concentrations as high as 0.04 mM do not inhibit glucose oxidation by NAD+
Sulfolobus solfataricus
N-ethylmaleimide
-
Sulfolobus solfataricus
NADPH
inhibits the NAD+-dependent carbohydrate oxidations in a competitive manner with respect to NAD+
Sulfolobus solfataricus
tetrahydrofuran
-
Sulfolobus solfataricus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.03
-
NADP+
in the presence of D-galactose, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70C; in the presence of D-glucose, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70C; in the presence of D-xylose, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70C
Sulfolobus solfataricus
0.44
-
D-glucose
in the presence of NADP+, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70C
Sulfolobus solfataricus
1.2
-
NAD+
in the presence of D-glucose, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70C; in the presence of D-xylose, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70C
Sulfolobus solfataricus
2.2
-
D-xylose
in the presence of NADP+, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70C
Sulfolobus solfataricus
8
-
D-glucose
in the presence of NAD+, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70C
Sulfolobus solfataricus
22
-
D-galactose
in the presence of NADP+, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70C
Sulfolobus solfataricus
68
-
D-xylose
in the presence of NAD+, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70C
Sulfolobus solfataricus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
maximally active at 20 mM
Sulfolobus solfataricus
Mg2+
maximally active at 20 mM
Sulfolobus solfataricus
Mn2+
maximally active at 20 mM
Sulfolobus solfataricus
additional information
Zn2+ is partially effective, whereas Ni2+, Cd2+ and univalent cations are ineffective in promoting enzyme reactivation
Sulfolobus solfataricus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
30100
-
4 * 30100, gel filtration, in the presence of 5% (w/v) SDS
Sulfolobus solfataricus
60200
-
2 * 60200, gel filtration, in the presence of 6 M guanidinium chloride
Sulfolobus solfataricus
124000
-
gel filtration
Sulfolobus solfataricus
Organic Solvent Stability
Organic Solvent
Commentary
Organism
Acetone
stable in the presence of chaotropic agents and water-miscible organic solvents such as acetone
Sulfolobus solfataricus
Ethanol
a slow time-dependent decrease of activity is observed in the presence of ethanol
Sulfolobus solfataricus
Methanol
stable in the presence of chaotropic agents and water-miscible organic solvents such as methanol
Sulfolobus solfataricus
additional information
2 M urea and 0.05% (w/v) SDS do not cause any loss of enzymic activity
Sulfolobus solfataricus
SDS
a slow time-dependent decrease of activity is observed in the presence of 0.1% SDS
Sulfolobus solfataricus
urea
a slow time-dependent decrease of activity is observed in the presence of 4 M urea
Sulfolobus solfataricus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Sulfolobus solfataricus
-
strain MT-4, previously named Caldariella acidofila
-
Sulfolobus solfataricus MT-4
-
strain MT-4, previously named Caldariella acidofila
-
Purification (Commentary)
Commentary
Organism
SP-Sephadex C-50 gel filtration, phenyl-Sepharose column chromatography, and Affi-Gel Blue matrix gel filtration
Sulfolobus solfataricus
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.7
-
unpurified enzyme, at 70C
Sulfolobus solfataricus
437
-
after 623fold purification, at 70C
Sulfolobus solfataricus
Storage Stability
Storage Stability
Organism
37C, in the presence of 20 mM MgCl2 and 20% (v/v) ethylene glycol, 40 days, 50% loss of activity
Sulfolobus solfataricus
4C, in the presence of 20 mM MgCl2 and 20% (v/v) ethylene glycol, several months, remains stable
Sulfolobus solfataricus
70C, in the presence of 20 mM MgCl2 and 20% (v/v) ethylene glycol, 45 h, 50% loss of activity
Sulfolobus solfataricus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-amino-2-deoxy-D-glucose + NAD+
26% activity compared to D-glucose in combination with NAD+
639110
Sulfolobus solfataricus
? + NADH
-
-
-
?
2-amino-2-deoxy-D-glucose + NADP+
5% activity compared to D-glucose in combination with NAD+
639110
Sulfolobus solfataricus
? + NADPH
-
-
-
?
2-deoxy-D-glucose + NAD+
12% activity compared to D-glucose in combination with NAD+
639110
Sulfolobus solfataricus
2-deoxy-D-glucono-1,5-lactone + NADH + H+
-
-
-
?
2-deoxy-D-glucose + NAD+
12% activity compared to D-glucose in combination with NAD+
639110
Sulfolobus solfataricus MT-4
2-deoxy-D-glucono-1,5-lactone + NADH + H+
-
-
-
?
2-deoxy-D-glucose + NADP+
25% activity compared to D-glucose in combination with NAD+
639110
Sulfolobus solfataricus
? + NADPH
-
-
-
?
6-deoxy-D-glucose + NAD+
66% activity compared to D-glucose in combination with NAD+
639110
Sulfolobus solfataricus
6-deoxy-D-glucono-1,5-lactone + NADH + H+
-
-
-
?
6-deoxy-D-glucose + NAD+
66% activity compared to D-glucose in combination with NAD+
639110
Sulfolobus solfataricus MT-4
6-deoxy-D-glucono-1,5-lactone + NADH + H+
-
-
-
?
6-deoxy-D-glucose + NADP+
9% activity compared to D-glucose in combination with NAD+
639110
Sulfolobus solfataricus
? + NADPH
-
-
-
?
D-altrose + NAD+
5% activity compared to D-glucose in combination with NAD+
639110
Sulfolobus solfataricus
D-altrono-1,5-lactone + NADH + H+
-
-
-
?
D-altrose + NADP+
12% activity compared to D-glucose in combination with NAD+
639110
Sulfolobus solfataricus
? + NADPH
-
-
-
?
D-galactose + NADH
15% activity compared to D-glucose in combination with NAD+
639110
Sulfolobus solfataricus
D-galactono-1,5-lactone + NADH + H+
-
-
-
?
D-glucose + NAD+
100% activity
639110
Sulfolobus solfataricus
D-glucono-1,5-lactone + NADH + H+
-
-
-
?
D-glucose + NAD+
100% activity
639110
Sulfolobus solfataricus MT-4
D-glucono-1,5-lactone + NADH + H+
-
-
-
?
D-glucose + NADP+
9% activity compared to D-glucose in combination with NAD+
639110
Sulfolobus solfataricus
D-glucono-1,5-lactone + NADPH + H+
-
-
-
?
D-glucose + NADP+
9% activity compared to D-glucose in combination with NAD+
639110
Sulfolobus solfataricus MT-4
D-glucono-1,5-lactone + NADPH + H+
-
-
-
?
D-gulose + NAD+
8% activity compared to D-glucose in combination with NAD+
639110
Sulfolobus solfataricus
? + NADH
-
-
-
?
D-gulose + NADP+
12% activity compared to D-glucose in combination with NAD+
639110
Sulfolobus solfataricus
? + NADPH
-
-
-
?
D-idose + NAD+
65% activity compared to D-glucose in combination with NAD+
639110
Sulfolobus solfataricus
? + NADH
-
-
-
?
D-idose + NADP+
12% activity compared to D-glucose in combination with NAD+
639110
Sulfolobus solfataricus
? + NADPH
-
-
-
?
D-mannose + NADP+
4% activity compared to D-glucose in combination with NAD+
639110
Sulfolobus solfataricus
? + NADPH
-
-
-
?
D-ribose + NADP+
4% activity compared to D-glucose in combination with NAD+
639110
Sulfolobus solfataricus
? + NADPH
-
-
-
?
D-xylose + NAD+
26% activity compared to D-glucose in combination with NAD+
639110
Sulfolobus solfataricus
? + NADH
-
-
-
?
D-xylose + NADP+
28% activity compared to D-glucose in combination with NAD+
639110
Sulfolobus solfataricus
? + NADPH
-
-
-
?
additional information
no activity towards D-mannose,D-galactose, and D-ribose in combination with NAD+
639110
Sulfolobus solfataricus
?
-
-
-
-
additional information
no activity towards D-mannose,D-galactose, and D-ribose in combination with NAD+
639110
Sulfolobus solfataricus MT-4
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
homodimer
2 * 60200, gel filtration, in the presence of 6 M guanidinium chloride
Sulfolobus solfataricus
homotetramer
4 * 30100, gel filtration, in the presence of 5% (w/v) SDS
Sulfolobus solfataricus
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
77
-
-
Sulfolobus solfataricus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Sulfolobus solfataricus
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
5
9
the enzyme stability does not vary significantly in the pH range 5-9
Sulfolobus solfataricus
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Sulfolobus solfataricus
NADP+
-
Sulfolobus solfataricus
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.075
-
NADPH
using D-glucose as a substrate
Sulfolobus solfataricus
IC50 Value
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
1
-
at 25C and pH 9
Sulfolobus solfataricus
N-ethylmaleimide
3
-
at 25C and pH 9
Sulfolobus solfataricus
5,5'-dithiobis-(2-nitrobenzoic acid)
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Sulfolobus solfataricus
NADP+
-
Sulfolobus solfataricus
IC50 Value (protein specific)
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
1
-
at 25C and pH 9
Sulfolobus solfataricus
N-ethylmaleimide
3
-
at 25C and pH 9
Sulfolobus solfataricus
5,5'-dithiobis-(2-nitrobenzoic acid)
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2-mercaptoethanol
rapid inactivation at 1 mM
Sulfolobus solfataricus
5,5'-dithiobis-(2-nitrobenzoic acid)
-
Sulfolobus solfataricus
EDTA
5% residual activity at 50 mM, addition of Mg2+ in excess restored the initial activity
Sulfolobus solfataricus
Guanidinium chloride
rapid inactivation at 4 M
Sulfolobus solfataricus
additional information
D-alactose, D-mannose and D-ribose, in concentrations as high as 0.04 mM do not inhibit glucose oxidation by NAD+
Sulfolobus solfataricus
N-ethylmaleimide
-
Sulfolobus solfataricus
NADPH
inhibits the NAD+-dependent carbohydrate oxidations in a competitive manner with respect to NAD+
Sulfolobus solfataricus
tetrahydrofuran
-
Sulfolobus solfataricus
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.075
-
NADPH
using D-glucose as a substrate
Sulfolobus solfataricus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.03
-
NADP+
in the presence of D-galactose, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70C; in the presence of D-glucose, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70C; in the presence of D-xylose, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70C
Sulfolobus solfataricus
0.44
-
D-glucose
in the presence of NADP+, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70C
Sulfolobus solfataricus
1.2
-
NAD+
in the presence of D-glucose, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70C; in the presence of D-xylose, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70C
Sulfolobus solfataricus
2.2
-
D-xylose
in the presence of NADP+, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70C
Sulfolobus solfataricus
8
-
D-glucose
in the presence of NAD+, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70C
Sulfolobus solfataricus
22
-
D-galactose
in the presence of NADP+, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70C
Sulfolobus solfataricus
68
-
D-xylose
in the presence of NAD+, in 100 mM triethanolamine/HCl buffer, 20 mM MgCl2, at 70C
Sulfolobus solfataricus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
maximally active at 20 mM
Sulfolobus solfataricus
Mg2+
maximally active at 20 mM
Sulfolobus solfataricus
Mn2+
maximally active at 20 mM
Sulfolobus solfataricus
additional information
Zn2+ is partially effective, whereas Ni2+, Cd2+ and univalent cations are ineffective in promoting enzyme reactivation
Sulfolobus solfataricus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
30100
-
4 * 30100, gel filtration, in the presence of 5% (w/v) SDS
Sulfolobus solfataricus
60200
-
2 * 60200, gel filtration, in the presence of 6 M guanidinium chloride
Sulfolobus solfataricus
124000
-
gel filtration
Sulfolobus solfataricus
Organic Solvent Stability (protein specific)
Organic Solvent
Commentary
Organism
Acetone
stable in the presence of chaotropic agents and water-miscible organic solvents such as acetone
Sulfolobus solfataricus
Ethanol
a slow time-dependent decrease of activity is observed in the presence of ethanol
Sulfolobus solfataricus
Methanol
stable in the presence of chaotropic agents and water-miscible organic solvents such as methanol
Sulfolobus solfataricus
additional information
2 M urea and 0.05% (w/v) SDS do not cause any loss of enzymic activity
Sulfolobus solfataricus
SDS
a slow time-dependent decrease of activity is observed in the presence of 0.1% SDS
Sulfolobus solfataricus
urea
a slow time-dependent decrease of activity is observed in the presence of 4 M urea
Sulfolobus solfataricus
Purification (Commentary) (protein specific)
Commentary
Organism
SP-Sephadex C-50 gel filtration, phenyl-Sepharose column chromatography, and Affi-Gel Blue matrix gel filtration
Sulfolobus solfataricus
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.7
-
unpurified enzyme, at 70C
Sulfolobus solfataricus
437
-
after 623fold purification, at 70C
Sulfolobus solfataricus
Storage Stability (protein specific)
Storage Stability
Organism
37C, in the presence of 20 mM MgCl2 and 20% (v/v) ethylene glycol, 40 days, 50% loss of activity
Sulfolobus solfataricus
4C, in the presence of 20 mM MgCl2 and 20% (v/v) ethylene glycol, several months, remains stable
Sulfolobus solfataricus
70C, in the presence of 20 mM MgCl2 and 20% (v/v) ethylene glycol, 45 h, 50% loss of activity
Sulfolobus solfataricus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-amino-2-deoxy-D-glucose + NAD+
26% activity compared to D-glucose in combination with NAD+
639110
Sulfolobus solfataricus
? + NADH
-
-
-
?
2-amino-2-deoxy-D-glucose + NADP+
5% activity compared to D-glucose in combination with NAD+
639110
Sulfolobus solfataricus
? + NADPH
-
-
-
?
2-deoxy-D-glucose + NAD+
12% activity compared to D-glucose in combination with NAD+
639110
Sulfolobus solfataricus
2-deoxy-D-glucono-1,5-lactone + NADH + H+
-
-
-
?
2-deoxy-D-glucose + NAD+
12% activity compared to D-glucose in combination with NAD+
639110
Sulfolobus solfataricus MT-4
2-deoxy-D-glucono-1,5-lactone + NADH + H+
-
-
-
?
2-deoxy-D-glucose + NADP+
25% activity compared to D-glucose in combination with NAD+
639110
Sulfolobus solfataricus
? + NADPH
-
-
-
?
6-deoxy-D-glucose + NAD+
66% activity compared to D-glucose in combination with NAD+
639110
Sulfolobus solfataricus
6-deoxy-D-glucono-1,5-lactone + NADH + H+
-
-
-
?
6-deoxy-D-glucose + NAD+
66% activity compared to D-glucose in combination with NAD+
639110
Sulfolobus solfataricus MT-4
6-deoxy-D-glucono-1,5-lactone + NADH + H+
-
-
-
?
6-deoxy-D-glucose + NADP+
9% activity compared to D-glucose in combination with NAD+
639110
Sulfolobus solfataricus
? + NADPH
-
-
-
?
D-altrose + NAD+
5% activity compared to D-glucose in combination with NAD+
639110
Sulfolobus solfataricus
D-altrono-1,5-lactone + NADH + H+
-
-
-
?
D-altrose + NADP+
12% activity compared to D-glucose in combination with NAD+
639110
Sulfolobus solfataricus
? + NADPH
-
-
-
?
D-galactose + NADH
15% activity compared to D-glucose in combination with NAD+
639110
Sulfolobus solfataricus
D-galactono-1,5-lactone + NADH + H+
-
-
-
?
D-glucose + NAD+
100% activity
639110
Sulfolobus solfataricus
D-glucono-1,5-lactone + NADH + H+
-
-
-
?
D-glucose + NAD+
100% activity
639110
Sulfolobus solfataricus MT-4
D-glucono-1,5-lactone + NADH + H+
-
-
-
?
D-glucose + NADP+
9% activity compared to D-glucose in combination with NAD+
639110
Sulfolobus solfataricus
D-glucono-1,5-lactone + NADPH + H+
-
-
-
?
D-glucose + NADP+
9% activity compared to D-glucose in combination with NAD+
639110
Sulfolobus solfataricus MT-4
D-glucono-1,5-lactone + NADPH + H+
-
-
-
?
D-gulose + NAD+
8% activity compared to D-glucose in combination with NAD+
639110
Sulfolobus solfataricus
? + NADH
-
-
-
?
D-gulose + NADP+
12% activity compared to D-glucose in combination with NAD+
639110
Sulfolobus solfataricus
? + NADPH
-
-
-
?
D-idose + NAD+
65% activity compared to D-glucose in combination with NAD+
639110
Sulfolobus solfataricus
? + NADH
-
-
-
?
D-idose + NADP+
12% activity compared to D-glucose in combination with NAD+
639110
Sulfolobus solfataricus
? + NADPH
-
-
-
?
D-mannose + NADP+
4% activity compared to D-glucose in combination with NAD+
639110
Sulfolobus solfataricus
? + NADPH
-
-
-
?
D-ribose + NADP+
4% activity compared to D-glucose in combination with NAD+
639110
Sulfolobus solfataricus
? + NADPH
-
-
-
?
D-xylose + NAD+
26% activity compared to D-glucose in combination with NAD+
639110
Sulfolobus solfataricus
? + NADH
-
-
-
?
D-xylose + NADP+
28% activity compared to D-glucose in combination with NAD+
639110
Sulfolobus solfataricus
? + NADPH
-
-
-
?
additional information
no activity towards D-mannose,D-galactose, and D-ribose in combination with NAD+
639110
Sulfolobus solfataricus
?
-
-
-
-
additional information
no activity towards D-mannose,D-galactose, and D-ribose in combination with NAD+
639110
Sulfolobus solfataricus MT-4
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
2 * 60200, gel filtration, in the presence of 6 M guanidinium chloride
Sulfolobus solfataricus
homotetramer
4 * 30100, gel filtration, in the presence of 5% (w/v) SDS
Sulfolobus solfataricus
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
77
-
-
Sulfolobus solfataricus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Sulfolobus solfataricus
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
5
9
the enzyme stability does not vary significantly in the pH range 5-9
Sulfolobus solfataricus
Other publictions for EC 1.1.1.118
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739713
Zhang
Efficient 9alpha-hydroxy-4-and ...
Bacillus subtilis 168, Bacillus subtilis
SpringerPlus
5
1207
2016
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2
-
72
-
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-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
701045
Vazquez-Figueroa
Thermostable variants construc ...
Bacillus subtilis 168, Bacillus subtilis
Protein Eng. Des. Sel.
21
673-680
2008
-
-
1
-
4
-
-
-
-
-
-
-
-
72
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
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-
-
-
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-
-
-
-
-
-
-
668049
Manjon
-
Increased activity of glucose ...
Komagataeibacter xylinus
Biotechnol. Lett.
24
1227-1232
2002
-
1
-
-
-
-
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-
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-
-
1
-
1
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-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
285779
Lin
Continuous production of L-car ...
Pseudomonas putida, Pseudomonas putida IAM 12014
J. Biosci. Bioeng.
87
361-364
1999
-
1
-
-
-
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-
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3
-
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2
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-
-
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1
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-
-
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-
-
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-
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-
2
-
-
-
-
-
-
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-
639110
Giardina
Glucose dehydrogenase from the ...
Sulfolobus solfataricus, Sulfolobus solfataricus MT-4
Biochem. J.
239
517-522
1986
-
-
-
-
-
-
8
7
-
4
3
-
6
20
-
-
1
-
-
-
2
3
25
2
1
-
-
-
1
-
1
2
1
-
2
-
-
-
2
-
-
-
2
8
1
7
-
4
3
-
6
-
-
1
-
-
2
3
25
2
1
-
-
-
1
-
1
-
-
-
-
-
-
-
285777
Kobayashi
-
Identification and growth char ...
Corynebacterium sp., Corynebacterium sp. No. 93-1
Agric. Biol. Chem.
44
41-47
1980
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2
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2
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1
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1
-
1
-
1
1
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-
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-
1
-
-
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-
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-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
1
-
1
-
1
-
-
-
-
-
-
-
285778
Anderson
D-Aldohexose dehydrogenase ...
Pseudomonas sp., Pseudomonas sp. MSU-1
Methods Enzymol.
41
147-150
1975
-
-
-
-
-
-
-
3
-
-
-
-
-
8
-
-
1
-
-
-
1
1
16
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
3
-
-
-
-
-
-
-
1
-
-
1
1
16
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
285776
Hu
The regulation of some sugar d ...
Pseudomonas sp.
Biochim. Biophys. Acta
93
237-245
1964
-
-
-
-
-
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-
-
-
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1
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-
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2
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-
1
-
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-
-
1
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-
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-
-
-
-
-
-
-
-
-
-
-
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2
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