BRENDA - Enzyme Database show
show all sequences of 1.1.1.117

Some physical properties of three sugar dehydrogenases from a pseudomonad

Cline, A.L.; Hu, A.S.L.; J. Biol. Chem. 240, 4498-4502 (1965)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.2
-
NADP+
-
Pseudomonas sp.
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Pseudomonas sp.
5737
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
20000
-
x * 20000, sedimentation analysis in 4.2 M guanidinium chloride, presence of subunits of unequal size might be possible
Pseudomonas sp.
50000
-
gel filtration
Pseudomonas sp.
104000
-
sedimentation analysis in EDTA/phosphate buffer
Pseudomonas sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-arabinose + NAD(P)+
Pseudomonas sp.
-
D-arabino-1,4-lactone + NAD(P)H
-
-
?
D-arabinose + NAD(P)+
Pseudomonas sp. G6
-
D-arabino-1,4-lactone + NAD(P)H
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas sp.
-
G6
-
Pseudomonas sp. G6
-
G6
-
Purification (Commentary)
Commentary
Organism
-
Pseudomonas sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-arabinose + NAD(P)+
-
285797
Pseudomonas sp.
D-arabino-1,4-lactone + NAD(P)H
-
-
-
?
D-arabinose + NAD(P)+
-
285797
Pseudomonas sp.
D-arabino-1,4-lactone + NAD(P)H
-
285797
Pseudomonas sp.
?
D-arabinose + NAD(P)+
-
285797
Pseudomonas sp. G6
D-arabino-1,4-lactone + NAD(P)H
-
-
-
?
D-arabinose + NAD(P)+
-
285797
Pseudomonas sp. G6
D-arabino-1,4-lactone + NAD(P)H
-
285797
Pseudomonas sp. G6
?
Subunits
Subunits
Commentary
Organism
?
x * 20000, sedimentation analysis in 4.2 M guanidinium chloride, presence of subunits of unequal size might be possible
Pseudomonas sp.
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
58
-
half-life of 20 s is increased to 80 s in presence of cofactor, in both cases biphasic inactivation curve
Pseudomonas sp.
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Pseudomonas sp.
NADP+
-
Pseudomonas sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Pseudomonas sp.
NADP+
-
Pseudomonas sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.2
-
NADP+
-
Pseudomonas sp.
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Pseudomonas sp.
5737
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
20000
-
x * 20000, sedimentation analysis in 4.2 M guanidinium chloride, presence of subunits of unequal size might be possible
Pseudomonas sp.
50000
-
gel filtration
Pseudomonas sp.
104000
-
sedimentation analysis in EDTA/phosphate buffer
Pseudomonas sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-arabinose + NAD(P)+
Pseudomonas sp.
-
D-arabino-1,4-lactone + NAD(P)H
-
-
?
D-arabinose + NAD(P)+
Pseudomonas sp. G6
-
D-arabino-1,4-lactone + NAD(P)H
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-arabinose + NAD(P)+
-
285797
Pseudomonas sp.
D-arabino-1,4-lactone + NAD(P)H
-
-
-
?
D-arabinose + NAD(P)+
-
285797
Pseudomonas sp.
D-arabino-1,4-lactone + NAD(P)H
-
285797
Pseudomonas sp.
?
D-arabinose + NAD(P)+
-
285797
Pseudomonas sp. G6
D-arabino-1,4-lactone + NAD(P)H
-
-
-
?
D-arabinose + NAD(P)+
-
285797
Pseudomonas sp. G6
D-arabino-1,4-lactone + NAD(P)H
-
285797
Pseudomonas sp. G6
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 20000, sedimentation analysis in 4.2 M guanidinium chloride, presence of subunits of unequal size might be possible
Pseudomonas sp.
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
58
-
half-life of 20 s is increased to 80 s in presence of cofactor, in both cases biphasic inactivation curve
Pseudomonas sp.
Other publictions for EC 1.1.1.117
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
710944
Gonzalez
Role of Saccharomyces cerevisi ...
Saccharomyces cerevisiae
Appl. Environ. Microbiol.
76
670-679
2010
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
285774
Kim
D-Arabinose dehydrogenase and ...
Saccharomyces cerevisiae
Biochim. Biophys. Acta
1429
29-39
1998
-
-
-
-
-
-
-
4
1
-
2
1
-
1
-
-
1
-
-
-
-
-
5
1
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
4
1
-
2
1
-
-
-
1
-
-
-
-
5
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
10663
Kim
D-Arabinose dehydrogenase and ...
Candida albicans
Biochim. Biophys. Acta
1297
1-8
1996
-
-
-
-
-
-
7
6
1
-
2
1
-
1
-
-
1
-
-
-
-
-
5
1
1
-
-
-
1
-
-
1
1
-
-
-
-
-
1
-
-
-
-
7
1
6
1
-
2
1
-
-
-
1
-
-
-
-
5
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
389443
Metzger
Partial purification of rat li ...
Rattus norvegicus
Biochem. Biophys. Res. Commun.
26
742-747
1967
-
-
-
-
-
-
-
2
1
-
-
1
-
1
-
-
1
-
-
1
-
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
2
1
-
-
1
-
-
-
1
-
1
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
285790
Cline
The isolation of three sugar d ...
Pseudomonas sp., Pseudomonas sp. G6
J. Biol. Chem.
240
4488-4492
1965
-
-
-
-
-
-
-
-
1
-
-
2
-
5
-
-
1
-
-
-
1
4
3
-
1
-
1
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
-
2
-
-
-
1
-
-
1
4
3
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
285791
Cline
Enzymatic characterization and ...
Pseudomonas sp., Pseudomonas sp. G6
J. Biol. Chem.
240
4493-4497
1965
-
-
-
-
-
-
-
3
1
-
-
1
-
5
-
-
1
-
-
-
-
-
5
-
1
-
-
-
1
1
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
3
1
-
-
1
-
-
-
1
-
-
-
-
5
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
285797
Cline
Some physical properties of th ...
Pseudomonas sp., Pseudomonas sp. G6
J. Biol. Chem.
240
4498-4502
1965
-
-
-
-
-
-
-
1
1
-
3
2
-
5
-
-
1
-
-
-
-
-
4
1
-
-
1
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
1
1
-
3
2
-
-
-
1
-
-
-
-
4
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-