BRENDA - Enzyme Database show
show all sequences of 1.1.1.112

Monkey liver indanol dehydrogenase. Purification, properties, and kinetic mechanism

Hara, A.; Mouri, K.; Nakagawa, M.; Nakamura, M.; Nakayama, T.; Matsuura, K.; Saweda, H.; J. Biochem. 106, 126-132 (1989)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
competitive inhibition vs. indanol
Macaca fuscata
2,2'-bipyridine
competitive inhibition vs. indanol
Macaca fuscata
4,4'-Bipyridine
competitive inhibition vs. indanol
Macaca fuscata
8-hydroxyquinoline
competitive inhibition vs. indanol
Macaca fuscata
Cibacron blue
noncompetitive inhibition vs. indanol
Macaca fuscata
Hexestrol
competitive inhibition vs. indanol
Macaca fuscata
Medroxyprogesterone acetate
competitive inhibition vs. indanol
Macaca fuscata
progesterone
competitive inhibition vs. indanol
Macaca fuscata
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
36000
-
1 * 36000, SDS-PAGE
Macaca fuscata
38000
-
gel filtration
Macaca fuscata
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Macaca fuscata
-
-
-
Purification (Commentary)
Commentary
Organism
ammonium sulfate, Sephadex G-100, Blue Sepharose, hydroxyapatite, chromatofocusing
Macaca fuscata
Reaction
Reaction
Commentary
Organism
indan-1-ol + NAD(P)+ = indanone + NAD(P)H + H+
A-stereospecific enzyme, ping-pong mechanism
Macaca fuscata
Source Tissue
Source Tissue
Commentary
Organism
Textmining
brain
-
Macaca fuscata
-
heart
-
Macaca fuscata
-
kidney
-
Macaca fuscata
-
liver
-
Macaca fuscata
-
lung
-
Macaca fuscata
-
spleen
-
Macaca fuscata
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
11.1
-
1-acenaphthenol as substrate
Macaca fuscata
Storage Stability
Storage Stability
Organism
4°C, 150 mM KCl, more than 1 mg/ml enzyme concentration, 4 months, little loss of activity
Macaca fuscata
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,2,3,4-tetrahydro-1-naphthol + NAD(P)+
-
285758
Macaca fuscata
1,2,3,4-tetrahydro-1-naphthone + NAD(P)H
-
285758
Macaca fuscata
?
1-acenaphthenol + NAD(P)+
-
285758
Macaca fuscata
1-acenaphthenone + NAD(P)H
-
285758
Macaca fuscata
?
2,3-butanedione + NAD(P)H
-
285758
Macaca fuscata
2,3-butanediol + NAD(P)+
-
285758
Macaca fuscata
?
2,3-heptanedione + NAD(P)H
-
285758
Macaca fuscata
2,3-heptanediol + NAD(P)+
-
285758
Macaca fuscata
?
2-nitrobenzaldehyde + NAD(P)H
-
285758
Macaca fuscata
2-nitrobenzyl alcohol + NAD(P)+
-
285758
Macaca fuscata
?
3-nitrobenzaldehyde + NAD(P)H
-
285758
Macaca fuscata
3-nitrobenzyl alcohol + NAD(P)+
-
285758
Macaca fuscata
?
4-nitrobenzaldehyde + NAD(P)H
-
285758
Macaca fuscata
4-nitrobenzyl alcohol + NAD(P)+
-
285758
Macaca fuscata
?
benzene dihydrodiol + NAD(P)+
-
285758
Macaca fuscata
o-benzoquinone + NAD(P)H
-
285758
Macaca fuscata
?
camphorquinone + NAD(P)H + H+
-
285758
Macaca fuscata
camphor-1,4-diol + NAD(P)+
-
285758
Macaca fuscata
?
cyclohex-2-en-1-ol + NAD(P)+
-
285758
Macaca fuscata
cyclohex-2-en-1-one + NAD(P)H
-
285758
Macaca fuscata
?
indan-(RS)-1-ol + NAD(P)+
-
285758
Macaca fuscata
indan-1-one + NAD(P)H
-
285758
Macaca fuscata
?
indan-(S)-1-ol + NAD(P)+
-
285758
Macaca fuscata
indan-1-one + NAD(P)H
-
285758
Macaca fuscata
?
indan-2-ol + NAD(P)+
-
285758
Macaca fuscata
indan-2-one + NAD(P)H
-
285758
Macaca fuscata
?
trans-1,2-dihydroxy-1,2-dihydronaphthalene + NAD(P)+
-
285758
Macaca fuscata
1,2-naphthoquinone + NAD(P)H
-
285758
Macaca fuscata
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 36000, SDS-PAGE
Macaca fuscata
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
15
50
linear increase
Macaca fuscata
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
48
-
60% activity lost after 10 min, pH 7.0
Macaca fuscata
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.4
-
-
Macaca fuscata
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
5.2
10.5
10 min at 25°C
Macaca fuscata
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Macaca fuscata
NADP+
5 times higher dehydrogenase rates compared to NAD+
Macaca fuscata
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Macaca fuscata
NADP+
5 times higher dehydrogenase rates compared to NAD+
Macaca fuscata
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
competitive inhibition vs. indanol
Macaca fuscata
2,2'-bipyridine
competitive inhibition vs. indanol
Macaca fuscata
4,4'-Bipyridine
competitive inhibition vs. indanol
Macaca fuscata
8-hydroxyquinoline
competitive inhibition vs. indanol
Macaca fuscata
Cibacron blue
noncompetitive inhibition vs. indanol
Macaca fuscata
Hexestrol
competitive inhibition vs. indanol
Macaca fuscata
Medroxyprogesterone acetate
competitive inhibition vs. indanol
Macaca fuscata
progesterone
competitive inhibition vs. indanol
Macaca fuscata
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
36000
-
1 * 36000, SDS-PAGE
Macaca fuscata
38000
-
gel filtration
Macaca fuscata
Purification (Commentary) (protein specific)
Commentary
Organism
ammonium sulfate, Sephadex G-100, Blue Sepharose, hydroxyapatite, chromatofocusing
Macaca fuscata
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
brain
-
Macaca fuscata
-
heart
-
Macaca fuscata
-
kidney
-
Macaca fuscata
-
liver
-
Macaca fuscata
-
lung
-
Macaca fuscata
-
spleen
-
Macaca fuscata
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
11.1
-
1-acenaphthenol as substrate
Macaca fuscata
Storage Stability (protein specific)
Storage Stability
Organism
4°C, 150 mM KCl, more than 1 mg/ml enzyme concentration, 4 months, little loss of activity
Macaca fuscata
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,2,3,4-tetrahydro-1-naphthol + NAD(P)+
-
285758
Macaca fuscata
1,2,3,4-tetrahydro-1-naphthone + NAD(P)H
-
285758
Macaca fuscata
?
1-acenaphthenol + NAD(P)+
-
285758
Macaca fuscata
1-acenaphthenone + NAD(P)H
-
285758
Macaca fuscata
?
2,3-butanedione + NAD(P)H
-
285758
Macaca fuscata
2,3-butanediol + NAD(P)+
-
285758
Macaca fuscata
?
2,3-heptanedione + NAD(P)H
-
285758
Macaca fuscata
2,3-heptanediol + NAD(P)+
-
285758
Macaca fuscata
?
2-nitrobenzaldehyde + NAD(P)H
-
285758
Macaca fuscata
2-nitrobenzyl alcohol + NAD(P)+
-
285758
Macaca fuscata
?
3-nitrobenzaldehyde + NAD(P)H
-
285758
Macaca fuscata
3-nitrobenzyl alcohol + NAD(P)+
-
285758
Macaca fuscata
?
4-nitrobenzaldehyde + NAD(P)H
-
285758
Macaca fuscata
4-nitrobenzyl alcohol + NAD(P)+
-
285758
Macaca fuscata
?
benzene dihydrodiol + NAD(P)+
-
285758
Macaca fuscata
o-benzoquinone + NAD(P)H
-
285758
Macaca fuscata
?
camphorquinone + NAD(P)H + H+
-
285758
Macaca fuscata
camphor-1,4-diol + NAD(P)+
-
285758
Macaca fuscata
?
cyclohex-2-en-1-ol + NAD(P)+
-
285758
Macaca fuscata
cyclohex-2-en-1-one + NAD(P)H
-
285758
Macaca fuscata
?
indan-(RS)-1-ol + NAD(P)+
-
285758
Macaca fuscata
indan-1-one + NAD(P)H
-
285758
Macaca fuscata
?
indan-(S)-1-ol + NAD(P)+
-
285758
Macaca fuscata
indan-1-one + NAD(P)H
-
285758
Macaca fuscata
?
indan-2-ol + NAD(P)+
-
285758
Macaca fuscata
indan-2-one + NAD(P)H
-
285758
Macaca fuscata
?
trans-1,2-dihydroxy-1,2-dihydronaphthalene + NAD(P)+
-
285758
Macaca fuscata
1,2-naphthoquinone + NAD(P)H
-
285758
Macaca fuscata
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 36000, SDS-PAGE
Macaca fuscata
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
15
50
linear increase
Macaca fuscata
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
48
-
60% activity lost after 10 min, pH 7.0
Macaca fuscata
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.4
-
-
Macaca fuscata
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
5.2
10.5
10 min at 25°C
Macaca fuscata
Other publictions for EC 1.1.1.112
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
285758
Hara
Monkey liver indanol dehydroge ...
Macaca fuscata
J. Biochem.
106
126-132
1989
-
-
-
-
-
-
8
-
1
-
2
-
-
2
-
-
1
1
-
7
1
1
14
1
-
1
1
-
1
-
1
2
-
-
-
-
-
-
2
-
-
-
-
8
-
-
1
-
2
-
-
-
-
1
-
7
1
1
14
1
-
1
1
-
1
-
1
-
-
-
-
-
-
-
285759
Hara
3(20)alpha-hydroxysteroi dehyd ...
Macaca fuscata
J. Biochem.
106
900-903
1989
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
3
-
-
18
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
18
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
285760
Hara
Isolation of multiple forms of ...
Oryctolagus cuniculus
Arch. Biochem. Biophys.
249
225-236
1986
-
-
-
-
-
-
8
-
1
-
6
-
-
2
-
-
1
-
-
2
7
-
29
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
8
-
-
1
-
6
-
-
-
-
1
-
2
7
-
29
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
285761
Kulkarni
Human placental indanol dehydr ...
Homo sapiens
Xenobiotica
15
513-519
1985
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
2
6
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
6
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
285762
Billings
The dehydrogenation of 1-indan ...
Bos taurus
J. Biol. Chem.
246
3512-3517
1971
-
-
-
-
-
-
4
-
-
-
-
-
-
1
-
-
1
-
-
3
1
-
8
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
2
-
-
-
-
4
-
-
-
-
-
-
-
-
-
1
-
3
1
-
8
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-