BRENDA - Enzyme Database show
show all sequences of 1.1.1.11

Identification, purification, and characterization of a D-arabinitol-specific dehydrogenase from Candida tropicalis

Quong, M.W.; Miyada, C.G.; Switchenko, A.C.; Goodman, T.C.; Biochem. Biophys. Res. Commun. 196, 1323-1329 (1993)

Data extracted from this reference:

General Stability
General Stability
Organism
Mg2+ stabilizes
Candida tropicalis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.12
-
NAD+
-
Candida tropicalis
39.8
-
D-arabinitol
-
Candida tropicalis
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
1-5 mM increases activity about 50%, also enhances stability
Candida tropicalis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
31000
-
x * 31000, SDS-PAGE
Candida tropicalis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-arabinitol + NAD+
Candida tropicalis
-
D-ribulose + NADH + H+
-
Candida tropicalis
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Candida tropicalis
-
-
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
96
-
-
Candida tropicalis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-arabinitol + NAD+
-
9566
Candida tropicalis
D-ribulose + NADH + H+
-
9566
Candida tropicalis
?
Galactitol + NAD+
1.6% activity
9566
Candida tropicalis
?
-
-
-
?
L-Xylulose + NADH
3% activity
9566
Candida tropicalis
?
-
-
-
?
additional information
no activity with D-mannitol
9566
Candida tropicalis
?
-
-
-
-
Xylitol + NAD+
5% activity
9566
Candida tropicalis
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 31000, SDS-PAGE
Candida tropicalis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
optimal activity, reduction
Candida tropicalis
pH Range
pH Minimum
pH Maximum
Commentary
Organism
10
11
enzyme activity increases with increasing pH, measurements above pH 11.0 not feasible
Candida tropicalis
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
6
9.5
more labile at increasing pH from 6.0-9.5
Candida tropicalis
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Candida tropicalis
NADH
-
Candida tropicalis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Candida tropicalis
NADH
-
Candida tropicalis
General Stability (protein specific)
General Stability
Organism
Mg2+ stabilizes
Candida tropicalis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.12
-
NAD+
-
Candida tropicalis
39.8
-
D-arabinitol
-
Candida tropicalis
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
1-5 mM increases activity about 50%, also enhances stability
Candida tropicalis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
31000
-
x * 31000, SDS-PAGE
Candida tropicalis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-arabinitol + NAD+
Candida tropicalis
-
D-ribulose + NADH + H+
-
Candida tropicalis
?
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
96
-
-
Candida tropicalis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-arabinitol + NAD+
-
9566
Candida tropicalis
D-ribulose + NADH + H+
-
9566
Candida tropicalis
?
Galactitol + NAD+
1.6% activity
9566
Candida tropicalis
?
-
-
-
?
L-Xylulose + NADH
3% activity
9566
Candida tropicalis
?
-
-
-
?
additional information
no activity with D-mannitol
9566
Candida tropicalis
?
-
-
-
-
Xylitol + NAD+
5% activity
9566
Candida tropicalis
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 31000, SDS-PAGE
Candida tropicalis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
optimal activity, reduction
Candida tropicalis
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
10
11
enzyme activity increases with increasing pH, measurements above pH 11.0 not feasible
Candida tropicalis
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
6
9.5
more labile at increasing pH from 6.0-9.5
Candida tropicalis
Other publictions for EC 1.1.1.11
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
701082
Cheng
Cloning, purification and char ...
Gluconobacter oxydans
Protein J.
28
263-272
2009
1
1
1
-
-
-
3
8
-
3
3
-
-
4
-
-
1
-
-
-
12
2
18
3
1
1
-
7
2
-
1
2
-
2
-
1
1
2
4
-
-
-
-
3
-
12
-
3
4
-
-
-
-
2
-
-
13
2
18
3
1
1
-
12
3
-
1
2
1
-
-
1
6
6
670549
LaFayette
Arabitol dehydrogenase as a se ...
Escherichia coli, Escherichia coli C
Plant Cell Rep.
24
596-602
2005
-
1
1
-
-
-
-
-
-
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285753
Murray
Isolation, characterization an ...
Candida tropicalis
Gene
155
123-128
1995
-
-
1
-
-
-
-
-
-
-
2
1
-
1
-
-
-
-
-
-
1
-
3
1
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
1
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285754
Hallborn
A short-chain dehydrogenase ge ...
Scheffersomyces stipitis
Yeast
11
839-847
1995
-
-
1
-
-
-
-
2
-
1
2
3
-
1
-
-
-
-
-
-
3
-
6
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
2
-
1
2
3
-
-
-
-
-
-
3
-
6
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
9565
Wong
D-Arabitol metabolism in Candi ...
Candida albicans, Candida albicans B331
J. Bacteriol.
175
6314-6320
1993
-
-
1
-
-
-
-
-
-
-
1
2
-
7
-
-
-
-
-
-
2
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
2
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
9566
Quong
Identification, purification, ...
Candida tropicalis
Biochem. Biophys. Res. Commun.
196
1323-1329
1993
-
-
-
-
-
1
-
2
-
1
1
1
-
1
-
-
-
-
-
-
1
-
5
1
-
-
-
-
1
1
1
2
-
-
-
-
-
-
2
-
-
1
-
-
-
2
-
1
1
1
-
-
-
-
-
-
1
-
5
1
-
-
-
-
1
1
1
-
-
-
-
-
-
-
285747
Neuberger
Purification and properties of ...
Enterobacter aerogenes
Biochem. J.
183
31-42
1979
-
-
1
-
-
1
-
3
-
-
4
1
-
4
-
-
1
-
-
-
1
1
2
1
1
-
1
-
-
-
-
1
-
-
-
-
-
1
1
-
-
1
-
-
-
3
-
-
4
1
-
-
-
1
-
-
1
1
2
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
285748
Wilson
Regulation of D-xylose and D-a ...
Enterobacter aerogenes, Enterobacter aerogenes PRL-R3
J. Bacteriol.
113
1404-1411
1973
-
-
-
-
-
-
-
-
-
-
-
2
-
7
-
-
-
-
-
-
1
-
2
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
285749
Fossitt
-
D-Arabitol dehydrogenase ...
Enterobacter aerogenes, Enterobacter aerogenes PRL-R3
Methods Enzymol.
9
184-187
1966
-
-
-
-
-
-
-
2
-
-
-
2
-
7
-
-
1
-
-
-
-
1
2
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
2
-
-
-
2
-
-
-
1
-
-
-
1
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
285750
Wood
Ribitol and D-arabitol utiliza ...
Enterobacter aerogenes, Enterobacter aerogenes PRL-R3
J. Biol. Chem.
236
2190-2195
1961
-
-
-
-
-
-
-
2
-
-
-
2
-
7
-
-
1
-
-
-
1
1
2
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
2
-
-
-
1
-
-
1
1
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
285751
Lin
An inducible D-arabitol dehydr ...
Enterobacter aerogenes
J. Biol. Chem.
236
31-36
1961
-
-
-
-
-
-
3
2
-
-
-
1
-
2
-
-
1
-
-
-
1
-
2
-
-
-
2
-
1
1
-
3
-
-
-
-
-
-
3
-
-
-
-
3
-
2
-
-
-
1
-
-
-
1
-
-
1
-
2
-
-
-
2
-
1
1
-
-
-
-
-
-
-
-