BRENDA - Enzyme Database show
show all sequences of 1.1.1.11

A short-chain dehydrogenase gene from Pichia stipitis having D-arabinitol dehydrogenase activity

Hallborn, J.; Walfridsson, M.; Penttilae, M.; Keraenen, S.; Hahn-Haegerdal, B.; Yeast 11, 839-847 (1995)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene over-expressed in Saccharomyces cerevisiae S700
Scheffersomyces stipitis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.2
-
NAD+
-
Scheffersomyces stipitis
20
-
D-arabinitol
-
Scheffersomyces stipitis
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
no divalent cations are required for activity
Scheffersomyces stipitis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
30000
-
4 * 30000, calculated from gene sequence and molecular weight of native enzyme
Scheffersomyces stipitis
110000
-
native gradient PAGE
Scheffersomyces stipitis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-arabinitol + NAD+
Scheffersomyces stipitis
recombinant protein from Saccharomyces cerevisiae
D-ribulose + NADH + H+
-
Scheffersomyces stipitis
?
D-arabinitol + NAD+
Scheffersomyces stipitis
D-arabitol i.e. D-arabinitol
D-xylulose + NADH + H+
-
Scheffersomyces stipitis
-
additional information
Scheffersomyces stipitis
D-arabinitol dehydrogenases (ArDH) from fungi and yeast oxidize D-arabinitol to D-ribulose, whereas bacterial ArDH oxidizes D-arabinitol to D-xylulose
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Scheffersomyces stipitis
-
strain CBS 6054
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
0.2
-
D-xylulose, of recombinant protein
Scheffersomyces stipitis
0.78
-
-
Scheffersomyces stipitis
68.25
-
D-arabinitol, of recombinant protein
Scheffersomyces stipitis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-arabinitol + NAD+
recombinant protein from Saccharomyces cerevisiae
285754
Scheffersomyces stipitis
D-ribulose + NADH + H+
-
285754
Scheffersomyces stipitis
?
D-arabinitol + NAD+
D-arabitol i.e. D-arabinitol
285754
Scheffersomyces stipitis
D-xylulose + NADH + H+
-
285754
Scheffersomyces stipitis
-
D-glucitol + NAD+
recombinant protein from Saccharomyces cerevisiae and enzyme from Pichia stipitis
285754
Scheffersomyces stipitis
?
-
-
-
?
Galactitol + NAD+
recombinant protein from Saccharomyces cerevisiae
285754
Scheffersomyces stipitis
?
-
-
-
?
additional information
D-arabinitol dehydrogenases (ArDH) from fungi and yeast oxidize D-arabinitol to D-ribulose, whereas bacterial ArDH oxidizes D-arabinitol to D-xylulose
285754
Scheffersomyces stipitis
?
-
-
-
-
Xylitol + NAD+
recombinant protein from Saccharomyces cerevisiae and enzyme from Pichia stipitis
285754
Scheffersomyces stipitis
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
tetramer
4 * 30000, calculated from gene sequence and molecular weight of native enzyme
Scheffersomyces stipitis
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
no activity with NADPH
Scheffersomyces stipitis
Cloned(Commentary) (protein specific)
Commentary
Organism
gene over-expressed in Saccharomyces cerevisiae S700
Scheffersomyces stipitis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
no activity with NADPH
Scheffersomyces stipitis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.2
-
NAD+
-
Scheffersomyces stipitis
20
-
D-arabinitol
-
Scheffersomyces stipitis
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
no divalent cations are required for activity
Scheffersomyces stipitis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
30000
-
4 * 30000, calculated from gene sequence and molecular weight of native enzyme
Scheffersomyces stipitis
110000
-
native gradient PAGE
Scheffersomyces stipitis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-arabinitol + NAD+
Scheffersomyces stipitis
recombinant protein from Saccharomyces cerevisiae
D-ribulose + NADH + H+
-
Scheffersomyces stipitis
?
D-arabinitol + NAD+
Scheffersomyces stipitis
D-arabitol i.e. D-arabinitol
D-xylulose + NADH + H+
-
Scheffersomyces stipitis
-
additional information
Scheffersomyces stipitis
D-arabinitol dehydrogenases (ArDH) from fungi and yeast oxidize D-arabinitol to D-ribulose, whereas bacterial ArDH oxidizes D-arabinitol to D-xylulose
?
-
-
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
0.2
-
D-xylulose, of recombinant protein
Scheffersomyces stipitis
0.78
-
-
Scheffersomyces stipitis
68.25
-
D-arabinitol, of recombinant protein
Scheffersomyces stipitis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-arabinitol + NAD+
recombinant protein from Saccharomyces cerevisiae
285754
Scheffersomyces stipitis
D-ribulose + NADH + H+
-
285754
Scheffersomyces stipitis
?
D-arabinitol + NAD+
D-arabitol i.e. D-arabinitol
285754
Scheffersomyces stipitis
D-xylulose + NADH + H+
-
285754
Scheffersomyces stipitis
-
D-glucitol + NAD+
recombinant protein from Saccharomyces cerevisiae and enzyme from Pichia stipitis
285754
Scheffersomyces stipitis
?
-
-
-
?
Galactitol + NAD+
recombinant protein from Saccharomyces cerevisiae
285754
Scheffersomyces stipitis
?
-
-
-
?
additional information
D-arabinitol dehydrogenases (ArDH) from fungi and yeast oxidize D-arabinitol to D-ribulose, whereas bacterial ArDH oxidizes D-arabinitol to D-xylulose
285754
Scheffersomyces stipitis
?
-
-
-
-
Xylitol + NAD+
recombinant protein from Saccharomyces cerevisiae and enzyme from Pichia stipitis
285754
Scheffersomyces stipitis
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 30000, calculated from gene sequence and molecular weight of native enzyme
Scheffersomyces stipitis
Other publictions for EC 1.1.1.11
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
701082
Cheng
Cloning, purification and char ...
Gluconobacter oxydans
Protein J.
28
263-272
2009
1
1
1
-
-
-
3
8
-
3
3
-
-
4
-
-
1
-
-
-
12
2
18
3
1
1
-
7
2
-
1
2
-
2
-
1
1
2
4
-
-
-
-
3
-
12
-
3
4
-
-
-
-
2
-
-
13
2
18
3
1
1
-
12
3
-
1
2
1
-
-
1
6
6
670549
LaFayette
Arabitol dehydrogenase as a se ...
Escherichia coli, Escherichia coli C
Plant Cell Rep.
24
596-602
2005
-
1
1
-
-
-
-
-
-
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285753
Murray
Isolation, characterization an ...
Candida tropicalis
Gene
155
123-128
1995
-
-
1
-
-
-
-
-
-
-
2
1
-
1
-
-
-
-
-
-
1
-
3
1
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
1
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285754
Hallborn
A short-chain dehydrogenase ge ...
Scheffersomyces stipitis
Yeast
11
839-847
1995
-
-
1
-
-
-
-
2
-
1
2
3
-
1
-
-
-
-
-
-
3
-
6
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
2
-
1
2
3
-
-
-
-
-
-
3
-
6
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
9565
Wong
D-Arabitol metabolism in Candi ...
Candida albicans, Candida albicans B331
J. Bacteriol.
175
6314-6320
1993
-
-
1
-
-
-
-
-
-
-
1
2
-
7
-
-
-
-
-
-
2
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
2
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
9566
Quong
Identification, purification, ...
Candida tropicalis
Biochem. Biophys. Res. Commun.
196
1323-1329
1993
-
-
-
-
-
1
-
2
-
1
1
1
-
1
-
-
-
-
-
-
1
-
5
1
-
-
-
-
1
1
1
2
-
-
-
-
-
-
2
-
-
1
-
-
-
2
-
1
1
1
-
-
-
-
-
-
1
-
5
1
-
-
-
-
1
1
1
-
-
-
-
-
-
-
285747
Neuberger
Purification and properties of ...
Enterobacter aerogenes
Biochem. J.
183
31-42
1979
-
-
1
-
-
1
-
3
-
-
4
1
-
4
-
-
1
-
-
-
1
1
2
1
1
-
1
-
-
-
-
1
-
-
-
-
-
1
1
-
-
1
-
-
-
3
-
-
4
1
-
-
-
1
-
-
1
1
2
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
285748
Wilson
Regulation of D-xylose and D-a ...
Enterobacter aerogenes, Enterobacter aerogenes PRL-R3
J. Bacteriol.
113
1404-1411
1973
-
-
-
-
-
-
-
-
-
-
-
2
-
7
-
-
-
-
-
-
1
-
2
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
285749
Fossitt
-
D-Arabitol dehydrogenase ...
Enterobacter aerogenes, Enterobacter aerogenes PRL-R3
Methods Enzymol.
9
184-187
1966
-
-
-
-
-
-
-
2
-
-
-
2
-
7
-
-
1
-
-
-
-
1
2
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
2
-
-
-
2
-
-
-
1
-
-
-
1
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
285750
Wood
Ribitol and D-arabitol utiliza ...
Enterobacter aerogenes, Enterobacter aerogenes PRL-R3
J. Biol. Chem.
236
2190-2195
1961
-
-
-
-
-
-
-
2
-
-
-
2
-
7
-
-
1
-
-
-
1
1
2
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
2
-
-
-
1
-
-
1
1
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
285751
Lin
An inducible D-arabitol dehydr ...
Enterobacter aerogenes
J. Biol. Chem.
236
31-36
1961
-
-
-
-
-
-
3
2
-
-
-
1
-
2
-
-
1
-
-
-
1
-
2
-
-
-
2
-
1
1
-
3
-
-
-
-
-
-
3
-
-
-
-
3
-
2
-
-
-
1
-
-
-
1
-
-
1
-
2
-
-
-
2
-
1
1
-
-
-
-
-
-
-
-