BRENDA - Enzyme Database show
show all sequences of 1.1.1.11

An inducible D-arabitol dehydrogenase from Aerobacter aerogenes

Lin, E.C.C.; J. Biol. Chem. 236, 31-36 (1961)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
2 mM, 37% inhibition
Klebsiella aerogenes
2,2'-dipyridyl
2 mM, 19% inhibition
Klebsiella aerogenes
diethyldithiocarbamate
10 mM, 28% inhibition
Klebsiella aerogenes
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5
-
D-arabinitol
-
Klebsiella aerogenes
6.1
-
D-mannitol
-
Klebsiella aerogenes
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-arabinitol + NAD+
Klebsiella aerogenes
-
D-xylulose + NADH + H+
-
Klebsiella aerogenes
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Klebsiella aerogenes
-
strain P14 (guanine auxotroph), derived from strain 1033
-
Purification (Commentary)
Commentary
Organism
strain P14
Klebsiella aerogenes
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
7.5
-
-
Klebsiella aerogenes
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-arabinitol + NAD+
-
285751
Klebsiella aerogenes
D-xylulose + NADH + H+
-
285751
Klebsiella aerogenes
r
D-mannitol + NAD+
-
285751
Klebsiella aerogenes
D-fructose + NADH + H+
-
285751
Klebsiella aerogenes
?
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
43
-
half-life: 50 min
Klebsiella aerogenes
45
-
half-life: 7 min
Klebsiella aerogenes
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
D-arabinitol, D-mannitol
Klebsiella aerogenes
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7.5
10
at pH 7.5 and 10.0 about 50% relative activity, D-arabinitol
Klebsiella aerogenes
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
no activity with NADPH
Klebsiella aerogenes
NAD+
-
Klebsiella aerogenes
NADH
-
Klebsiella aerogenes
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
no activity with NADPH
Klebsiella aerogenes
NAD+
-
Klebsiella aerogenes
NADH
-
Klebsiella aerogenes
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
2 mM, 37% inhibition
Klebsiella aerogenes
2,2'-dipyridyl
2 mM, 19% inhibition
Klebsiella aerogenes
diethyldithiocarbamate
10 mM, 28% inhibition
Klebsiella aerogenes
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5
-
D-arabinitol
-
Klebsiella aerogenes
6.1
-
D-mannitol
-
Klebsiella aerogenes
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-arabinitol + NAD+
Klebsiella aerogenes
-
D-xylulose + NADH + H+
-
Klebsiella aerogenes
r
Purification (Commentary) (protein specific)
Commentary
Organism
strain P14
Klebsiella aerogenes
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
7.5
-
-
Klebsiella aerogenes
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-arabinitol + NAD+
-
285751
Klebsiella aerogenes
D-xylulose + NADH + H+
-
285751
Klebsiella aerogenes
r
D-mannitol + NAD+
-
285751
Klebsiella aerogenes
D-fructose + NADH + H+
-
285751
Klebsiella aerogenes
?
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
43
-
half-life: 50 min
Klebsiella aerogenes
45
-
half-life: 7 min
Klebsiella aerogenes
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
D-arabinitol, D-mannitol
Klebsiella aerogenes
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7.5
10
at pH 7.5 and 10.0 about 50% relative activity, D-arabinitol
Klebsiella aerogenes
Other publictions for EC 1.1.1.11
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
740490
Khan
Metabolic engineering of the c ...
Saccharomyces cerevisiae
Front. Plant Sci.
6
725
2015
-
2
1
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
2
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
701082
Cheng
Cloning, purification and char ...
Gluconobacter oxydans
Protein J.
28
263-272
2009
1
1
1
-
-
-
3
8
-
3
3
-
-
4
-
-
1
-
-
-
12
2
18
3
1
1
-
7
2
-
1
2
-
2
-
1
1
2
4
-
-
-
-
3
-
12
-
3
4
-
-
-
-
2
-
-
13
2
18
3
1
1
-
12
3
-
1
2
1
-
-
1
6
6
670549
LaFayette
Arabitol dehydrogenase as a se ...
Escherichia coli, Escherichia coli C
Plant Cell Rep.
24
596-602
2005
-
1
1
-
-
-
-
-
-
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285753
Murray
Isolation, characterization an ...
Candida tropicalis
Gene
155
123-128
1995
-
-
1
-
-
-
-
-
-
-
2
1
-
1
-
-
-
-
-
-
1
-
3
1
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
1
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285754
Hallborn
A short-chain dehydrogenase ge ...
Scheffersomyces stipitis
Yeast
11
839-847
1995
-
-
1
-
-
-
-
2
-
1
2
3
-
1
-
-
-
-
-
-
3
-
6
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
2
-
1
2
3
-
-
-
-
-
-
3
-
6
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
9565
Wong
D-Arabitol metabolism in Candi ...
Candida albicans, Candida albicans B331
J. Bacteriol.
175
6314-6320
1993
-
-
1
-
-
-
-
-
-
-
1
2
-
6
-
-
-
-
-
-
2
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
2
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
9566
Quong
Identification, purification, ...
Candida tropicalis
Biochem. Biophys. Res. Commun.
196
1323-1329
1993
-
-
-
-
-
1
-
2
-
1
1
1
-
1
-
-
-
-
-
-
1
-
5
1
-
-
-
-
1
1
1
2
-
-
-
-
-
-
2
-
-
1
-
-
-
2
-
1
1
1
-
-
-
-
-
-
1
-
5
1
-
-
-
-
1
1
1
-
-
-
-
-
-
-
285747
Neuberger
Purification and properties of ...
Klebsiella aerogenes
Biochem. J.
183
31-42
1979
-
-
1
-
-
1
-
3
-
-
4
1
-
4
-
-
1
-
-
-
1
1
2
1
1
-
1
-
-
-
-
1
-
-
-
-
-
1
1
-
-
1
-
-
-
3
-
-
4
1
-
-
-
1
-
-
1
1
2
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
285748
Wilson
Regulation of D-xylose and D-a ...
Klebsiella aerogenes, Klebsiella aerogenes PRL-R3
J. Bacteriol.
113
1404-1411
1973
-
-
-
-
-
-
-
-
-
-
-
2
-
7
-
-
-
-
-
-
1
-
2
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
285749
Fossitt
-
D-Arabitol dehydrogenase ...
Klebsiella aerogenes, Klebsiella aerogenes PRL-R3
Methods Enzymol.
9
184-187
1966
-
-
-
-
-
-
-
2
-
-
-
2
-
7
-
-
1
-
-
-
-
1
2
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
2
-
-
-
2
-
-
-
1
-
-
-
1
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
285750
Wood
Ribitol and D-arabitol utiliza ...
Klebsiella aerogenes, Klebsiella aerogenes PRL-R3
J. Biol. Chem.
236
2190-2195
1961
-
-
-
-
-
-
-
2
-
-
-
2
-
7
-
-
1
-
-
-
1
1
2
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
2
-
-
-
1
-
-
1
1
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
285751
Lin
An inducible D-arabitol dehydr ...
Klebsiella aerogenes
J. Biol. Chem.
236
31-36
1961
-
-
-
-
-
-
3
2
-
-
-
1
-
2
-
-
1
-
-
-
1
-
2
-
-
-
2
-
1
1
-
3
-
-
-
-
-
-
3
-
-
-
-
3
-
2
-
-
-
1
-
-
-
1
-
-
1
-
2
-
-
-
2
-
1
1
-
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-
-
-
-
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