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show all sequences of 1.1.1.11

Purification and properties of Klebsiella aerogenes D-arabitol dehydrogenase

Neuberger, M.S.; Patterson, R.A.; Hartley, B.S.; Biochem. J. 183, 31-42 (1979)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene over-expressed in Escherichia coli K12
Enterobacter aerogenes
General Stability
General Stability
Organism
glycerol stabilizes
Enterobacter aerogenes
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.04
-
NAD+
-
Enterobacter aerogenes
20
-
D-arabinitol
-
Enterobacter aerogenes
70
-
D-mannitol
-
Enterobacter aerogenes
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
43000
-
gel filtration, ultracentrifugation
Enterobacter aerogenes
44000
-
sedimentation equilibrium
Enterobacter aerogenes
46000
-
calculated from amino acid composition
Enterobacter aerogenes
46500
-
1 * 46500, SDS-PAGE
Enterobacter aerogenes
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-arabinitol + NAD+
Enterobacter aerogenes
-
D-xylulose + NADH + H+
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Enterobacter aerogenes
-
i.e. Klebsiella pneumoniae, gene expression in Escherichia coli K12
-
Purification (Commentary)
Commentary
Organism
recombinant protein from Escherichia coli
Enterobacter aerogenes
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
37.1
-
-
Enterobacter aerogenes
Storage Stability
Storage Stability
Organism
-20°C, protein concentration 50 mg/ml, 50% v/v glycerol, stable 3 months, loss of activity less than 50%
Enterobacter aerogenes
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-arabinitol + NAD+
-
285747
Enterobacter aerogenes
D-xylulose + NADH + H+
-
-
-
-
D-mannitol + NAD+
-
285747
Enterobacter aerogenes
D-fructose + NADH + H+
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 46500, SDS-PAGE
Enterobacter aerogenes
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
28
-
enzyme assay at
Enterobacter aerogenes
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
45
-
20 min
Enterobacter aerogenes
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Enterobacter aerogenes
Cloned(Commentary) (protein specific)
Commentary
Organism
gene over-expressed in Escherichia coli K12
Enterobacter aerogenes
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Enterobacter aerogenes
General Stability (protein specific)
General Stability
Organism
glycerol stabilizes
Enterobacter aerogenes
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.04
-
NAD+
-
Enterobacter aerogenes
20
-
D-arabinitol
-
Enterobacter aerogenes
70
-
D-mannitol
-
Enterobacter aerogenes
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
43000
-
gel filtration, ultracentrifugation
Enterobacter aerogenes
44000
-
sedimentation equilibrium
Enterobacter aerogenes
46000
-
calculated from amino acid composition
Enterobacter aerogenes
46500
-
1 * 46500, SDS-PAGE
Enterobacter aerogenes
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-arabinitol + NAD+
Enterobacter aerogenes
-
D-xylulose + NADH + H+
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant protein from Escherichia coli
Enterobacter aerogenes
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
37.1
-
-
Enterobacter aerogenes
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, protein concentration 50 mg/ml, 50% v/v glycerol, stable 3 months, loss of activity less than 50%
Enterobacter aerogenes
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-arabinitol + NAD+
-
285747
Enterobacter aerogenes
D-xylulose + NADH + H+
-
-
-
-
D-mannitol + NAD+
-
285747
Enterobacter aerogenes
D-fructose + NADH + H+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 46500, SDS-PAGE
Enterobacter aerogenes
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
28
-
enzyme assay at
Enterobacter aerogenes
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
45
-
20 min
Enterobacter aerogenes
Other publictions for EC 1.1.1.11
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
701082
Cheng
Cloning, purification and char ...
Gluconobacter oxydans
Protein J.
28
263-272
2009
1
1
1
-
-
-
3
8
-
3
3
-
-
4
-
-
1
-
-
-
12
2
18
3
1
1
-
7
2
-
1
2
-
2
-
1
1
2
4
-
-
-
-
3
-
12
-
3
4
-
-
-
-
2
-
-
13
2
18
3
1
1
-
12
3
-
1
2
1
-
-
1
6
6
670549
LaFayette
Arabitol dehydrogenase as a se ...
Escherichia coli, Escherichia coli C
Plant Cell Rep.
24
596-602
2005
-
1
1
-
-
-
-
-
-
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285753
Murray
Isolation, characterization an ...
Candida tropicalis
Gene
155
123-128
1995
-
-
1
-
-
-
-
-
-
-
2
1
-
1
-
-
-
-
-
-
1
-
3
1
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
1
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285754
Hallborn
A short-chain dehydrogenase ge ...
Scheffersomyces stipitis
Yeast
11
839-847
1995
-
-
1
-
-
-
-
2
-
1
2
3
-
1
-
-
-
-
-
-
3
-
6
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
2
-
1
2
3
-
-
-
-
-
-
3
-
6
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
9565
Wong
D-Arabitol metabolism in Candi ...
Candida albicans, Candida albicans B331
J. Bacteriol.
175
6314-6320
1993
-
-
1
-
-
-
-
-
-
-
1
2
-
7
-
-
-
-
-
-
2
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
2
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
9566
Quong
Identification, purification, ...
Candida tropicalis
Biochem. Biophys. Res. Commun.
196
1323-1329
1993
-
-
-
-
-
1
-
2
-
1
1
1
-
1
-
-
-
-
-
-
1
-
5
1
-
-
-
-
1
1
1
2
-
-
-
-
-
-
2
-
-
1
-
-
-
2
-
1
1
1
-
-
-
-
-
-
1
-
5
1
-
-
-
-
1
1
1
-
-
-
-
-
-
-
285747
Neuberger
Purification and properties of ...
Enterobacter aerogenes
Biochem. J.
183
31-42
1979
-
-
1
-
-
1
-
3
-
-
4
1
-
4
-
-
1
-
-
-
1
1
2
1
1
-
1
-
-
-
-
1
-
-
-
-
-
1
1
-
-
1
-
-
-
3
-
-
4
1
-
-
-
1
-
-
1
1
2
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
285748
Wilson
Regulation of D-xylose and D-a ...
Enterobacter aerogenes, Enterobacter aerogenes PRL-R3
J. Bacteriol.
113
1404-1411
1973
-
-
-
-
-
-
-
-
-
-
-
2
-
7
-
-
-
-
-
-
1
-
2
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
285749
Fossitt
-
D-Arabitol dehydrogenase ...
Enterobacter aerogenes, Enterobacter aerogenes PRL-R3
Methods Enzymol.
9
184-187
1966
-
-
-
-
-
-
-
2
-
-
-
2
-
7
-
-
1
-
-
-
-
1
2
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
2
-
-
-
2
-
-
-
1
-
-
-
1
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
285750
Wood
Ribitol and D-arabitol utiliza ...
Enterobacter aerogenes, Enterobacter aerogenes PRL-R3
J. Biol. Chem.
236
2190-2195
1961
-
-
-
-
-
-
-
2
-
-
-
2
-
7
-
-
1
-
-
-
1
1
2
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
2
-
-
-
1
-
-
1
1
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
285751
Lin
An inducible D-arabitol dehydr ...
Enterobacter aerogenes
J. Biol. Chem.
236
31-36
1961
-
-
-
-
-
-
3
2
-
-
-
1
-
2
-
-
1
-
-
-
1
-
2
-
-
-
2
-
1
1
-
3
-
-
-
-
-
-
3
-
-
-
-
3
-
2
-
-
-
1
-
-
-
1
-
-
1
-
2
-
-
-
2
-
1
1
-
-
-
-
-
-
-
-