BRENDA - Enzyme Database show
show all sequences of 1.1.1.108

Identification of genes required for Pseudomonas aeruginosa carnitine catabolism

Wargo, M.J.; Hogan, D.A.; Microbiology 155, 2411-2419 (2009)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
additional information
strain PA14 mutants with transposon insertions in PA5385 and PA5388 result in the inability of Pseudomonas aeruginosa to grow on carnitine, mutants from the PAO1 transposon insertions in each of the PA5388, PA5387, PA5386 and PA5388 genes are unable to grow on carnitine; strain PA14 mutants with transposon insertions in PA5385 and PA5388 result in the inability of Pseudomonas aeruginosa to grow on carnitine, mutants from the PAO1 transposon insertions in each of the PA5388, PA5387, PA5386 and PA5388 genes are unable to grow on carnitine; strain PA14 mutants with transposon insertions in PA5385 and PA5388 result in the inability of Pseudomonas aeruginosa to grow on carnitine, mutants from the PAO1 transposon insertions in each of the PA5388, PA5387, PA5386 and PA5388 genes are unable to grow on carnitine; strain PA14 mutants with transposon insertions in PA5385 and PA5388 result in the inability of Pseudomonas aeruginosa to grow on carnitine, mutants from the PAO1 transposon insertions in each of the PA5388, PA5387, PA5386 and PA5388 genes are unable to grow on carnitine
Pseudomonas aeruginosa
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas aeruginosa
Q9HTH5
PA5389; PA14 and PAO1 strains
-
Pseudomonas aeruginosa
Q9HTH6
PA5388; PA14 and PAO1 strains
-
Pseudomonas aeruginosa
Q9HTH7
PA5387; PA14 and PAO1 strains
-
Pseudomonas aeruginosa
Q9HTH8
PA5386; PA14 and PAO1 strains
-
Pseudomonas aeruginosa
Q9HTH9
PA5385; PA14 and PAO1 strains
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
carnitine + NAD+
the PA5388-PA5384 region contains the predicted carnitine dehydrogenase homologue along with other genes required for growth on carnitine. The PA5389 transcription factor (CDHR) is required for growth on carnitine, it is required for induction of the PA5388-PA5384 transcripts in response to carnitine
700060
Pseudomonas aeruginosa
3-dehydrocarnitine + NADH + H+
-
-
-
?
carnitine + NAD+
the PA5389 transcription factor (CDHR) is required for growth on carnitine, it is required for induction of the PA5388-PA5384 transcripts in response to carnitine
700060
Pseudomonas aeruginosa
3-dehydrocarnitine + NADH + H+
-
-
-
?
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
strain PA14 mutants with transposon insertions in PA5385 and PA5388 result in the inability of Pseudomonas aeruginosa to grow on carnitine, mutants from the PAO1 transposon insertions in each of the PA5388, PA5387, PA5386 and PA5388 genes are unable to grow on carnitine
Pseudomonas aeruginosa
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
carnitine + NAD+
the PA5388-PA5384 region contains the predicted carnitine dehydrogenase homologue along with other genes required for growth on carnitine. The PA5389 transcription factor (CDHR) is required for growth on carnitine, it is required for induction of the PA5388-PA5384 transcripts in response to carnitine
700060
Pseudomonas aeruginosa
3-dehydrocarnitine + NADH + H+
-
-
-
?
carnitine + NAD+
the PA5389 transcription factor (CDHR) is required for growth on carnitine, it is required for induction of the PA5388-PA5384 transcripts in response to carnitine
700060
Pseudomonas aeruginosa
3-dehydrocarnitine + NADH + H+
-
-
-
?
Other publictions for EC 1.1.1.108
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
711514
Arima
Biochemical characterization o ...
Rhizobium sp., Xanthomonas campestris, Xanthomonas campestris pv. translucens
Biosci. Biotechnol. Biochem.
74
1237-1242
2010
-
-
2
-
2
-
-
6
-
-
-
-
-
3
-
1
-
-
-
-
3
-
6
-
-
-
3
-
-
-
-
-
-
-
-
-
-
2
-
-
2
-
-
-
-
6
-
-
-
-
-
-
1
-
-
-
3
-
6
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
700060
Wargo
Identification of genes requir ...
Pseudomonas aeruginosa
Microbiology
155
2411-2419
2009
-
-
-
-
1
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
670345
Uanschou
-
What to learn from a comparati ...
Bacteria
Monatsh. Chem.
136
1365-1381
2005
-
1
1
-
-
-
-
-
-
-
1
1
-
1
-
-
-
-
-
1
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
1
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285779
Lin
Continuous production of L-car ...
Pseudomonas putida
J. Biosci. Bioeng.
87
361-364
1999
-
1
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285737
Hanschmann
Purification and properties of ...
Agrobacterium sp., Alcaligenes sp., Pseudomonas putida
Biochim. Biophys. Acta
1290
177-183
1996
-
-
-
-
-
-
3
8
-
-
3
3
-
4
-
-
1
-
-
-
1
-
7
1
2
-
-
-
3
-
-
3
-
-
-
-
-
-
3
-
-
-
-
3
-
8
-
-
3
3
-
-
-
1
-
-
1
-
7
1
2
-
-
-
3
-
-
-
-
-
-
-
-
-
285738
Hanschmann
-
Occurence of carnitine dehydro ...
Agrobacterium sp.
FEMS Microbiol. Lett.
119
371-376
1994
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
2
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285739
Mori
-
Purification and properties of ...
Rhizobium sp. YS-240
J. Ferment. Bioeng.
78
337-340
1994
-
-
-
-
-
-
11
4
-
-
2
1
-
1
-
-
1
-
-
-
1
-
2
1
1
-
1
-
2
-
1
1
-
-
-
-
-
-
1
-
-
-
-
11
-
4
-
-
2
1
-
-
-
1
-
-
1
-
2
1
1
-
1
-
2
-
1
-
-
-
-
-
-
-
285740
Matsumoto
Fluorometric determination of ...
Pseudomonas aeruginosa
Clin. Chem.
36
2072-2076
1990
-
1
-
-
-
-
-
2
-
-
-
1
-
2
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285741
Goulas
Purification and properties of ...
Pseudomonas putida
Biochim. Biophys. Acta
957
335-339
1988
-
-
-
-
-
-
-
6
-
-
2
1
-
2
-
-
1
-
-
-
2
-
2
1
1
1
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
6
-
-
2
1
-
-
-
1
-
-
2
-
2
1
1
1
-
-
2
-
-
-
-
-
-
-
-
-
285742
Mori
-
Purification and some properti ...
Xanthomonas translucens
Agric. Biol. Chem.
52
249-250
1988
-
-
-
-
-
-
10
4
-
-
2
1
-
1
-
-
1
-
-
-
1
-
2
1
-
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
10
-
4
-
-
2
1
-
-
-
1
-
-
1
-
2
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
285743
Vandecasteele
Enzymatic synthesis of L-carni ...
Pseudomonas aeruginosa, Pseudomonas putida
Appl. Environ. Microbiol.
39
327-334
1980
-
-
-
-
-
-
-
3
-
-
-
2
-
2
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
3
-
-
-
2
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285744
Schnpp
Kinetische Untersuchungen zum ...
Pseudomonas aeruginosa
Eur. J. Biochem.
10
56-60
1969
-
-
-
-
-
-
2
-
-
-
-
1
-
1
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285745
Aurich
Reinigung und Eigenschaften de ...
Pseudomonas aeruginosa
Eur. J. Biochem.
6
196-201
1968
-
-
-
-
-
-
-
5
-
-
-
1
-
1
-
-
1
-
-
-
1
-
2
-
1
-
1
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
5
-
-
-
1
-
-
-
1
-
-
1
-
2
-
1
-
1
-
2
-
-
-
-
-
-
-
-
-
285746
Aurich
An inducible carnitine dehydro ...
Pseudomonas aeruginosa
Biochim. Biophys. Acta
139
505-507
1967
-
-
-
-
-
-
4
2
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
4
-
2
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-