BRENDA - Enzyme Database show
show all sequences of 1.1.1.108

Purification and some properties of carnitine dehydrogenase from Xanthomonas translucens

Mori, N.; Kasugai, T.; Kitamoto, Y.; Ichikawa, Y.; Agric. Biol. Chem. 52, 249-250 (1988)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Ag+
-
Xanthomonas translucens
Ca2+
-
Xanthomonas translucens
Co2+
-
Xanthomonas translucens
Cu2+
-
Xanthomonas translucens
Hg+
-
Xanthomonas translucens
Li+
-
Xanthomonas translucens
Mn2+
-
Xanthomonas translucens
Ni2+
strong inhibition
Xanthomonas translucens
p-chloromercuribenzoate
-
Xanthomonas translucens
Zn2+
-
Xanthomonas translucens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.04
-
NADH
-
Xanthomonas translucens
0.25
-
NAD+
-
Xanthomonas translucens
1.71
-
3-dehydrocarnitine
-
Xanthomonas translucens
10
-
L-carnitine
-
Xanthomonas translucens
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
37000
-
2 * 37000, SDS-PAGE
Xanthomonas translucens
74000
-
gel filtration
Xanthomonas translucens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(R)-3-hydroxy-4-trimethylaminobutyrate + NAD+
Xanthomonas translucens
-
3-dehydrocarnitine + NADH + H+
-
Xanthomonas translucens
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Xanthomonas translucens
-
-
-
Purification (Commentary)
Commentary
Organism
ammonium sulfate, DEAE-cellulose, Sephadex G-100, 5'-AMP Sepharose 4B, Sephadex G-100
Xanthomonas translucens
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
21
-
-
Xanthomonas translucens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R)-3-hydroxy-4-trimethylaminobutyrate + NAD+
-
285742
Xanthomonas translucens
3-dehydrocarnitine + NADH + H+
-
285742
Xanthomonas translucens
r
carnitine + NAD+
-
285742
Xanthomonas translucens
3-dehydrocarnitine + NADH + H+
-
285742
Xanthomonas translucens
r
Subunits
Subunits
Commentary
Organism
dimer
2 * 37000, SDS-PAGE
Xanthomonas translucens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
reduction of 3-dehydrocarnitine
Xanthomonas translucens
9.5
-
oxidation of L-carnitine
Xanthomonas translucens
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Xanthomonas translucens
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Xanthomonas translucens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ag+
-
Xanthomonas translucens
Ca2+
-
Xanthomonas translucens
Co2+
-
Xanthomonas translucens
Cu2+
-
Xanthomonas translucens
Hg+
-
Xanthomonas translucens
Li+
-
Xanthomonas translucens
Mn2+
-
Xanthomonas translucens
Ni2+
strong inhibition
Xanthomonas translucens
p-chloromercuribenzoate
-
Xanthomonas translucens
Zn2+
-
Xanthomonas translucens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.04
-
NADH
-
Xanthomonas translucens
0.25
-
NAD+
-
Xanthomonas translucens
1.71
-
3-dehydrocarnitine
-
Xanthomonas translucens
10
-
L-carnitine
-
Xanthomonas translucens
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
37000
-
2 * 37000, SDS-PAGE
Xanthomonas translucens
74000
-
gel filtration
Xanthomonas translucens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(R)-3-hydroxy-4-trimethylaminobutyrate + NAD+
Xanthomonas translucens
-
3-dehydrocarnitine + NADH + H+
-
Xanthomonas translucens
r
Purification (Commentary) (protein specific)
Commentary
Organism
ammonium sulfate, DEAE-cellulose, Sephadex G-100, 5'-AMP Sepharose 4B, Sephadex G-100
Xanthomonas translucens
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
21
-
-
Xanthomonas translucens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R)-3-hydroxy-4-trimethylaminobutyrate + NAD+
-
285742
Xanthomonas translucens
3-dehydrocarnitine + NADH + H+
-
285742
Xanthomonas translucens
r
carnitine + NAD+
-
285742
Xanthomonas translucens
3-dehydrocarnitine + NADH + H+
-
285742
Xanthomonas translucens
r
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 37000, SDS-PAGE
Xanthomonas translucens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
reduction of 3-dehydrocarnitine
Xanthomonas translucens
9.5
-
oxidation of L-carnitine
Xanthomonas translucens
Other publictions for EC 1.1.1.108
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
711514
Arima
Biochemical characterization o ...
Rhizobium sp., Xanthomonas campestris, Xanthomonas campestris pv. translucens
Biosci. Biotechnol. Biochem.
74
1237-1242
2010
-
-
2
-
2
-
-
6
-
-
-
-
-
3
-
1
-
-
-
-
3
-
6
-
-
-
3
-
-
-
-
-
-
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-
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2
-
-
2
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6
-
-
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-
-
1
-
-
-
3
-
6
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
700060
Wargo
Identification of genes requir ...
Pseudomonas aeruginosa
Microbiology
155
2411-2419
2009
-
-
-
-
1
-
-
-
-
-
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5
-
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5
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4
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5
-
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-
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670345
Uanschou
-
What to learn from a comparati ...
Bacteria
Monatsh. Chem.
136
1365-1381
2005
-
1
1
-
-
-
-
-
-
-
1
1
-
1
-
-
-
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-
1
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2
1
-
-
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-
-
-
-
1
-
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1
1
1
-
-
-
-
-
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-
-
1
1
-
-
-
-
-
1
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285779
Lin
Continuous production of L-car ...
Pseudomonas putida
J. Biosci. Bioeng.
87
361-364
1999
-
1
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
4
-
-
-
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-
1
-
-
-
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1
-
1
-
-
-
-
-
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-
-
-
-
2
-
-
-
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-
4
-
-
-
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-
-
-
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-
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-
-
-
-
-
285737
Hanschmann
Purification and properties of ...
Agrobacterium sp., Alcaligenes sp., Pseudomonas putida
Biochim. Biophys. Acta
1290
177-183
1996
-
-
-
-
-
-
3
8
-
-
3
3
-
4
-
-
1
-
-
-
1
-
7
1
2
-
-
-
3
-
-
3
-
-
-
-
-
-
3
-
-
-
-
3
-
8
-
-
3
3
-
-
-
1
-
-
1
-
7
1
2
-
-
-
3
-
-
-
-
-
-
-
-
-
285738
Hanschmann
-
Occurence of carnitine dehydro ...
Agrobacterium sp.
FEMS Microbiol. Lett.
119
371-376
1994
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
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2
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3
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1
-
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1
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1
-
-
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2
-
3
-
-
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-
-
-
-
-
-
-
-
-
-
-
285739
Mori
-
Purification and properties of ...
Rhizobium sp. YS-240
J. Ferment. Bioeng.
78
337-340
1994
-
-
-
-
-
-
11
4
-
-
2
1
-
1
-
-
1
-
-
-
1
-
2
1
1
-
1
-
2
-
1
1
-
-
-
-
-
-
1
-
-
-
-
11
-
4
-
-
2
1
-
-
-
1
-
-
1
-
2
1
1
-
1
-
2
-
1
-
-
-
-
-
-
-
285740
Matsumoto
Fluorometric determination of ...
Pseudomonas aeruginosa
Clin. Chem.
36
2072-2076
1990
-
1
-
-
-
-
-
2
-
-
-
1
-
2
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285741
Goulas
Purification and properties of ...
Pseudomonas putida
Biochim. Biophys. Acta
957
335-339
1988
-
-
-
-
-
-
-
6
-
-
2
1
-
2
-
-
1
-
-
-
2
-
2
1
1
1
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
6
-
-
2
1
-
-
-
1
-
-
2
-
2
1
1
1
-
-
2
-
-
-
-
-
-
-
-
-
285742
Mori
-
Purification and some properti ...
Xanthomonas translucens
Agric. Biol. Chem.
52
249-250
1988
-
-
-
-
-
-
10
4
-
-
2
1
-
1
-
-
1
-
-
-
1
-
2
1
-
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
10
-
4
-
-
2
1
-
-
-
1
-
-
1
-
2
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
285743
Vandecasteele
Enzymatic synthesis of L-carni ...
Pseudomonas aeruginosa, Pseudomonas putida
Appl. Environ. Microbiol.
39
327-334
1980
-
-
-
-
-
-
-
3
-
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2
-
2
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1
-
4
-
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-
-
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-
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2
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-
-
2
-
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3
-
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-
2
-
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-
1
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285744
Schnöpp
Kinetische Untersuchungen zum ...
Pseudomonas aeruginosa
Eur. J. Biochem.
10
56-60
1969
-
-
-
-
-
-
2
-
-
-
-
1
-
1
-
-
-
-
-
-
2
-
2
-
-
-
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-
1
-
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1
-
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2
-
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-
1
-
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-
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-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285745
Aurich
Reinigung und Eigenschaften de ...
Pseudomonas aeruginosa
Eur. J. Biochem.
6
196-201
1968
-
-
-
-
-
-
-
5
-
-
-
1
-
1
-
-
1
-
-
-
1
-
2
-
1
-
1
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
5
-
-
-
1
-
-
-
1
-
-
1
-
2
-
1
-
1
-
2
-
-
-
-
-
-
-
-
-
285746
Aurich
An inducible carnitine dehydro ...
Pseudomonas aeruginosa
Biochim. Biophys. Acta
139
505-507
1967
-
-
-
-
-
-
4
2
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
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-
1
-
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1
-
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-
1
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4
-
2
-
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-
1
-
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2
-
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1
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