BRENDA - Enzyme Database show
show all sequences of 1.1.1.108

Purification and properties of carnitine dehydrogenase from Pseudomonas putida

Goulas, P.; Biochim. Biophys. Acta 957, 335-339 (1988)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.013
-
NADH
potassium phosphate buffer, pH 7
Pseudomonas putida
0.067
-
NADH
Tris-HCl buffer, pH 9
Pseudomonas putida
0.2
-
NAD+
-
Pseudomonas putida
1.1
-
3-dehydrocarnitine
potassium phosphate buffer, pH 7
Pseudomonas putida
1.54
-
3-dehydrocarnitine
Tris-HCl buffer, pH 9
Pseudomonas putida
6.25
-
L-carnitine
-
Pseudomonas putida
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32000
-
2 * 32000, SDS-PAGE
Pseudomonas putida
60000
-
gel filtration
Pseudomonas putida
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(R)-3-hydroxy-4-trimethylaminobutyrate + NAD+
Pseudomonas putida
-
3-dehydrocarnitine + NADH + H+
-
Pseudomonas putida
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas putida
-
-
-
Purification (Commentary)
Commentary
Organism
ammonium sulfate, Sephadex G-200, 5'-AMP-Sepharose 4B, gel filtration
Pseudomonas putida
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
55.3
-
-
Pseudomonas putida
240
-
reduction of 3-dehydrocarnitine at pH 7-7.5
Pseudomonas putida
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R)-3-hydroxy-4-trimethylaminobutyrate + NAD+
-
285741
Pseudomonas putida
3-dehydrocarnitine + NADH + H+
-
285741
Pseudomonas putida
r
carnitine + NAD+
-
285741
Pseudomonas putida
3-dehydrocarnitine + NADH + H+
-
285741
Pseudomonas putida
r
Subunits
Subunits
Commentary
Organism
dimer
2 * 32000, SDS-PAGE
Pseudomonas putida
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
activity declines rapidly above 35C
Pseudomonas putida
Temperature Range [C]
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
10
30
-
Pseudomonas putida
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
7.5
reduction of 3-dehydrocarnitine
Pseudomonas putida
9
-
oxidation of L-carnitine
Pseudomonas putida
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Pseudomonas putida
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Pseudomonas putida
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.013
-
NADH
potassium phosphate buffer, pH 7
Pseudomonas putida
0.067
-
NADH
Tris-HCl buffer, pH 9
Pseudomonas putida
0.2
-
NAD+
-
Pseudomonas putida
1.1
-
3-dehydrocarnitine
potassium phosphate buffer, pH 7
Pseudomonas putida
1.54
-
3-dehydrocarnitine
Tris-HCl buffer, pH 9
Pseudomonas putida
6.25
-
L-carnitine
-
Pseudomonas putida
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32000
-
2 * 32000, SDS-PAGE
Pseudomonas putida
60000
-
gel filtration
Pseudomonas putida
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(R)-3-hydroxy-4-trimethylaminobutyrate + NAD+
Pseudomonas putida
-
3-dehydrocarnitine + NADH + H+
-
Pseudomonas putida
r
Purification (Commentary) (protein specific)
Commentary
Organism
ammonium sulfate, Sephadex G-200, 5'-AMP-Sepharose 4B, gel filtration
Pseudomonas putida
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
55.3
-
-
Pseudomonas putida
240
-
reduction of 3-dehydrocarnitine at pH 7-7.5
Pseudomonas putida
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R)-3-hydroxy-4-trimethylaminobutyrate + NAD+
-
285741
Pseudomonas putida
3-dehydrocarnitine + NADH + H+
-
285741
Pseudomonas putida
r
carnitine + NAD+
-
285741
Pseudomonas putida
3-dehydrocarnitine + NADH + H+
-
285741
Pseudomonas putida
r
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 32000, SDS-PAGE
Pseudomonas putida
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
activity declines rapidly above 35C
Pseudomonas putida
Temperature Range [C] (protein specific)
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
10
30
-
Pseudomonas putida
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
7.5
reduction of 3-dehydrocarnitine
Pseudomonas putida
9
-
oxidation of L-carnitine
Pseudomonas putida
Other publictions for EC 1.1.1.108
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
711514
Arima
Biochemical characterization o ...
Rhizobium sp., Xanthomonas campestris, Xanthomonas campestris pv. translucens
Biosci. Biotechnol. Biochem.
74
1237-1242
2010
-
-
2
-
2
-
-
6
-
-
-
-
-
3
-
1
-
-
-
-
3
-
6
-
-
-
3
-
-
-
-
-
-
-
-
-
-
2
-
-
2
-
-
-
-
6
-
-
-
-
-
-
1
-
-
-
3
-
6
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
700060
Wargo
Identification of genes requir ...
Pseudomonas aeruginosa
Microbiology
155
2411-2419
2009
-
-
-
-
1
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
5
-
-
-
-
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-
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-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
670345
Uanschou
-
What to learn from a comparati ...
Bacteria
Monatsh. Chem.
136
1365-1381
2005
-
1
1
-
-
-
-
-
-
-
1
1
-
1
-
-
-
-
-
1
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
1
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285779
Lin
Continuous production of L-car ...
Pseudomonas putida
J. Biosci. Bioeng.
87
361-364
1999
-
1
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285737
Hanschmann
Purification and properties of ...
Agrobacterium sp., Alcaligenes sp., Pseudomonas putida
Biochim. Biophys. Acta
1290
177-183
1996
-
-
-
-
-
-
3
8
-
-
3
3
-
4
-
-
1
-
-
-
1
-
7
1
2
-
-
-
3
-
-
3
-
-
-
-
-
-
3
-
-
-
-
3
-
8
-
-
3
3
-
-
-
1
-
-
1
-
7
1
2
-
-
-
3
-
-
-
-
-
-
-
-
-
285738
Hanschmann
-
Occurence of carnitine dehydro ...
Agrobacterium sp.
FEMS Microbiol. Lett.
119
371-376
1994
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
2
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
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-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285739
Mori
-
Purification and properties of ...
Rhizobium sp. YS-240
J. Ferment. Bioeng.
78
337-340
1994
-
-
-
-
-
-
11
4
-
-
2
1
-
1
-
-
1
-
-
-
1
-
2
1
1
-
1
-
2
-
1
1
-
-
-
-
-
-
1
-
-
-
-
11
-
4
-
-
2
1
-
-
-
1
-
-
1
-
2
1
1
-
1
-
2
-
1
-
-
-
-
-
-
-
285740
Matsumoto
Fluorometric determination of ...
Pseudomonas aeruginosa
Clin. Chem.
36
2072-2076
1990
-
1
-
-
-
-
-
2
-
-
-
1
-
2
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285741
Goulas
Purification and properties of ...
Pseudomonas putida
Biochim. Biophys. Acta
957
335-339
1988
-
-
-
-
-
-
-
6
-
-
2
1
-
2
-
-
1
-
-
-
2
-
2
1
1
1
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
6
-
-
2
1
-
-
-
1
-
-
2
-
2
1
1
1
-
-
2
-
-
-
-
-
-
-
-
-
285742
Mori
-
Purification and some properti ...
Xanthomonas translucens
Agric. Biol. Chem.
52
249-250
1988
-
-
-
-
-
-
10
4
-
-
2
1
-
1
-
-
1
-
-
-
1
-
2
1
-
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
10
-
4
-
-
2
1
-
-
-
1
-
-
1
-
2
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
285743
Vandecasteele
Enzymatic synthesis of L-carni ...
Pseudomonas aeruginosa, Pseudomonas putida
Appl. Environ. Microbiol.
39
327-334
1980
-
-
-
-
-
-
-
3
-
-
-
2
-
2
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
3
-
-
-
2
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285744
Schnpp
Kinetische Untersuchungen zum ...
Pseudomonas aeruginosa
Eur. J. Biochem.
10
56-60
1969
-
-
-
-
-
-
2
-
-
-
-
1
-
1
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
1
-
-
-
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-
-
1
-
-
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-
2
-
-
-
-
-
1
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285745
Aurich
Reinigung und Eigenschaften de ...
Pseudomonas aeruginosa
Eur. J. Biochem.
6
196-201
1968
-
-
-
-
-
-
-
5
-
-
-
1
-
1
-
-
1
-
-
-
1
-
2
-
1
-
1
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
5
-
-
-
1
-
-
-
1
-
-
1
-
2
-
1
-
1
-
2
-
-
-
-
-
-
-
-
-
285746
Aurich
An inducible carnitine dehydro ...
Pseudomonas aeruginosa
Biochim. Biophys. Acta
139
505-507
1967
-
-
-
-
-
-
4
2
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
1
-
-
-
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-
-
1
-
-
-
-
4
-
2
-
-
-
1
-
-
-
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-
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2
-
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1
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