BRENDA - Enzyme Database show
show all sequences of 1.1.1.108

Purification and properties of carnitine dehydrogenase from Pseudomonas sp. YS-240

Mori, N.; Mitsuzumi, H.; Kitamoto, Y.; J. Ferment. Bioeng. 78, 337-340 (1994)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
5,5'-dithiobis(2-nitrobenzoate)
-
Rhizobium sp. YS-240
Ag+
-
Rhizobium sp. YS-240
Ca2+
60-70% inhibition
Rhizobium sp. YS-240
Co2+
60-70% inhibition
Rhizobium sp. YS-240
Cu+
60-70% inhibition
Rhizobium sp. YS-240
Cu2+
-
Rhizobium sp. YS-240
Hg+
strong inhibition
Rhizobium sp. YS-240
Hg2+
-
Rhizobium sp. YS-240
o-phenanthroline
38% inhibition
Rhizobium sp. YS-240
p-chloromercuribenzoate
-
Rhizobium sp. YS-240
Zn2+
60-70% inhibition
Rhizobium sp. YS-240
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.019
-
NADH
-
Rhizobium sp. YS-240
0.1
-
NAD+
-
Rhizobium sp. YS-240
0.54
-
3-dehydrocarnitine
-
Rhizobium sp. YS-240
1.2
-
L-carnitine
-
Rhizobium sp. YS-240
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
50000
-
2 * 50000, SDS-PAGE
Rhizobium sp. YS-240
110000
-
gel filtration
Rhizobium sp. YS-240
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(R)-3-hydroxy-4-trimethylaminobutyrate + NAD+
Rhizobium sp. YS-240
-
3-dehydrocarnitine + NADH + H+
-
Rhizobium sp. YS-240
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rhizobium sp. YS-240
-
-
-
Purification (Commentary)
Commentary
Organism
DEAE-Toyopearl, ammonium sulfate, hydroxylapatite, Sephacryl S-200, hydroxylapatite
Rhizobium sp. YS-240
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
23.3
-
-
Rhizobium sp. YS-240
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R)-3-hydroxy-4-trimethylaminobutyrate + NAD+
-
285739
Rhizobium sp. YS-240
3-dehydrocarnitine + NADH + H+
-
285739
Rhizobium sp. YS-240
r
carnitine + NAD+
-
285739
Rhizobium sp. YS-240
3-dehydrocarnitine + NADH + H+
-
285739
Rhizobium sp. YS-240
r
Subunits
Subunits
Commentary
Organism
dimer
2 * 50000, SDS-PAGE
Rhizobium sp. YS-240
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
-
Rhizobium sp. YS-240
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
35
-
30 min in 100 mM potassium phosphate, pH 7.5, enzyme is rapidly inactivated above 40°C
Rhizobium sp. YS-240
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
8
reduction of 3-dehydrocarnitine
Rhizobium sp. YS-240
9.5
-
oxidation of L-carnitine
Rhizobium sp. YS-240
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
7
8
-
Rhizobium sp. YS-240
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Rhizobium sp. YS-240
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Rhizobium sp. YS-240
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
5,5'-dithiobis(2-nitrobenzoate)
-
Rhizobium sp. YS-240
Ag+
-
Rhizobium sp. YS-240
Ca2+
60-70% inhibition
Rhizobium sp. YS-240
Co2+
60-70% inhibition
Rhizobium sp. YS-240
Cu+
60-70% inhibition
Rhizobium sp. YS-240
Cu2+
-
Rhizobium sp. YS-240
Hg+
strong inhibition
Rhizobium sp. YS-240
Hg2+
-
Rhizobium sp. YS-240
o-phenanthroline
38% inhibition
Rhizobium sp. YS-240
p-chloromercuribenzoate
-
Rhizobium sp. YS-240
Zn2+
60-70% inhibition
Rhizobium sp. YS-240
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.019
-
NADH
-
Rhizobium sp. YS-240
0.1
-
NAD+
-
Rhizobium sp. YS-240
0.54
-
3-dehydrocarnitine
-
Rhizobium sp. YS-240
1.2
-
L-carnitine
-
Rhizobium sp. YS-240
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
50000
-
2 * 50000, SDS-PAGE
Rhizobium sp. YS-240
110000
-
gel filtration
Rhizobium sp. YS-240
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(R)-3-hydroxy-4-trimethylaminobutyrate + NAD+
Rhizobium sp. YS-240
-
3-dehydrocarnitine + NADH + H+
-
Rhizobium sp. YS-240
r
Purification (Commentary) (protein specific)
Commentary
Organism
DEAE-Toyopearl, ammonium sulfate, hydroxylapatite, Sephacryl S-200, hydroxylapatite
Rhizobium sp. YS-240
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
23.3
-
-
Rhizobium sp. YS-240
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R)-3-hydroxy-4-trimethylaminobutyrate + NAD+
-
285739
Rhizobium sp. YS-240
3-dehydrocarnitine + NADH + H+
-
285739
Rhizobium sp. YS-240
r
carnitine + NAD+
-
285739
Rhizobium sp. YS-240
3-dehydrocarnitine + NADH + H+
-
285739
Rhizobium sp. YS-240
r
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 50000, SDS-PAGE
Rhizobium sp. YS-240
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
-
Rhizobium sp. YS-240
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
35
-
30 min in 100 mM potassium phosphate, pH 7.5, enzyme is rapidly inactivated above 40°C
Rhizobium sp. YS-240
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
8
reduction of 3-dehydrocarnitine
Rhizobium sp. YS-240
9.5
-
oxidation of L-carnitine
Rhizobium sp. YS-240
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
7
8
-
Rhizobium sp. YS-240
Other publictions for EC 1.1.1.108
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
711514
Arima
Biochemical characterization o ...
Rhizobium sp., Xanthomonas campestris, Xanthomonas campestris pv. translucens
Biosci. Biotechnol. Biochem.
74
1237-1242
2010
-
-
2
-
2
-
-
6
-
-
-
-
-
3
-
1
-
-
-
-
3
-
6
-
-
-
3
-
-
-
-
-
-
-
-
-
-
2
-
-
2
-
-
-
-
6
-
-
-
-
-
-
1
-
-
-
3
-
6
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
700060
Wargo
Identification of genes requir ...
Pseudomonas aeruginosa
Microbiology
155
2411-2419
2009
-
-
-
-
1
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
670345
Uanschou
-
What to learn from a comparati ...
Bacteria
Monatsh. Chem.
136
1365-1381
2005
-
1
1
-
-
-
-
-
-
-
1
1
-
1
-
-
-
-
-
1
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
1
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285779
Lin
Continuous production of L-car ...
Pseudomonas putida
J. Biosci. Bioeng.
87
361-364
1999
-
1
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285737
Hanschmann
Purification and properties of ...
Agrobacterium sp., Alcaligenes sp., Pseudomonas putida
Biochim. Biophys. Acta
1290
177-183
1996
-
-
-
-
-
-
3
8
-
-
3
3
-
4
-
-
1
-
-
-
1
-
7
1
2
-
-
-
3
-
-
3
-
-
-
-
-
-
3
-
-
-
-
3
-
8
-
-
3
3
-
-
-
1
-
-
1
-
7
1
2
-
-
-
3
-
-
-
-
-
-
-
-
-
285738
Hanschmann
-
Occurence of carnitine dehydro ...
Agrobacterium sp.
FEMS Microbiol. Lett.
119
371-376
1994
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
2
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285739
Mori
-
Purification and properties of ...
Rhizobium sp. YS-240
J. Ferment. Bioeng.
78
337-340
1994
-
-
-
-
-
-
11
4
-
-
2
1
-
1
-
-
1
-
-
-
1
-
2
1
1
-
1
-
2
-
1
1
-
-
-
-
-
-
1
-
-
-
-
11
-
4
-
-
2
1
-
-
-
1
-
-
1
-
2
1
1
-
1
-
2
-
1
-
-
-
-
-
-
-
285740
Matsumoto
Fluorometric determination of ...
Pseudomonas aeruginosa
Clin. Chem.
36
2072-2076
1990
-
1
-
-
-
-
-
2
-
-
-
1
-
2
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285741
Goulas
Purification and properties of ...
Pseudomonas putida
Biochim. Biophys. Acta
957
335-339
1988
-
-
-
-
-
-
-
6
-
-
2
1
-
2
-
-
1
-
-
-
2
-
2
1
1
1
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
6
-
-
2
1
-
-
-
1
-
-
2
-
2
1
1
1
-
-
2
-
-
-
-
-
-
-
-
-
285742
Mori
-
Purification and some properti ...
Xanthomonas translucens
Agric. Biol. Chem.
52
249-250
1988
-
-
-
-
-
-
10
4
-
-
2
1
-
1
-
-
1
-
-
-
1
-
2
1
-
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
10
-
4
-
-
2
1
-
-
-
1
-
-
1
-
2
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
285743
Vandecasteele
Enzymatic synthesis of L-carni ...
Pseudomonas aeruginosa, Pseudomonas putida
Appl. Environ. Microbiol.
39
327-334
1980
-
-
-
-
-
-
-
3
-
-
-
2
-
2
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
3
-
-
-
2
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285744
Schnöpp
Kinetische Untersuchungen zum ...
Pseudomonas aeruginosa
Eur. J. Biochem.
10
56-60
1969
-
-
-
-
-
-
2
-
-
-
-
1
-
1
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285745
Aurich
Reinigung und Eigenschaften de ...
Pseudomonas aeruginosa
Eur. J. Biochem.
6
196-201
1968
-
-
-
-
-
-
-
5
-
-
-
1
-
1
-
-
1
-
-
-
1
-
2
-
1
-
1
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
5
-
-
-
1
-
-
-
1
-
-
1
-
2
-
1
-
1
-
2
-
-
-
-
-
-
-
-
-
285746
Aurich
An inducible carnitine dehydro ...
Pseudomonas aeruginosa
Biochim. Biophys. Acta
139
505-507
1967
-
-
-
-
-
-
4
2
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
4
-
2
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-