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Literature summary for 1.1.1.108 extracted from

  • Hanschmann, H.; Ehricht, R.; Kleber, H.P.
    Purification and properties of L(-)-carnitine dehydrogenase from Agrobacterium sp. (1996), Biochim. Biophys. Acta, 1290, 177-183.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ L-carnitine dehydrogenase, complete inhibition Agrobacterium sp.
Hg2+ L-carnitine dehydrogenase, complete inhibition Agrobacterium sp.
trimethylamine L-carnitine dehydrogenase, strong inhibition Agrobacterium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.018
-
NAD+ L-carnitine dehydrogenase, two different forms of the enzyme or two binding sites Agrobacterium sp.
0.032
-
NADH L-carnitine dehydrogenase Agrobacterium sp.
0.042
-
NAD+ L-carnitine dehydrogenase, two different forms of the enzyme or two binding sites Agrobacterium sp.
0.14
-
NAD+
-
Alcaligenes sp.
0.29
-
L-carnitine L-carnitine dehydrogenase, two different forms of the enzyme or two binding sites Agrobacterium sp.
0.72
-
3-dehydrocarnitine L-carnitine dehydrogenase Agrobacterium sp.
6.1
-
L-carnitine L-carnitine dehydrogenase, two different forms of the enzyme or two binding sites Agrobacterium sp.
9.3
-
L-carnitine
-
Alcaligenes sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51000
-
gel filtration Alcaligenes sp.
57000
-
2 * 57000, L-carnitine dehydrogenase, SDS-PAGE Agrobacterium sp.
114000
-
gel filtration, L-carnitine dehydrogenase Agrobacterium sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-3-hydroxy-4-trimethylaminobutyrate + NAD+ Pseudomonas putida
-
3-dehydrocarnitine + NADH + H+
-
r
(R)-3-hydroxy-4-trimethylaminobutyrate + NAD+ Alcaligenes sp.
-
3-dehydrocarnitine + NADH + H+
-
r
(R)-3-hydroxy-4-trimethylaminobutyrate + NAD+ Agrobacterium sp.
-
3-dehydrocarnitine + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Agrobacterium sp.
-
DSM 8888, D-carnitine- and L-carnitine dehydrogenases are induced by D-carnitine and L-carnitine respectively
-
Alcaligenes sp.
-
-
-
Pseudomonas putida
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sepharose FF, Blue-Sepharose, Superose 12HR, Fractogel TSK Butyl, L-carnitine dehydrogenase Agrobacterium sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
16.75
-
L-carnitine dehydrogenase Agrobacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-3-hydroxy-4-trimethylaminobutyrate + NAD+
-
Pseudomonas putida 3-dehydrocarnitine + NADH + H+
-
r
(R)-3-hydroxy-4-trimethylaminobutyrate + NAD+
-
Alcaligenes sp. 3-dehydrocarnitine + NADH + H+
-
r
(R)-3-hydroxy-4-trimethylaminobutyrate + NAD+
-
Agrobacterium sp. 3-dehydrocarnitine + NADH + H+
-
r
carnitine + NAD+
-
Pseudomonas putida 3-dehydrocarnitine + NADH + H+
-
r
carnitine + NAD+
-
Alcaligenes sp. 3-dehydrocarnitine + NADH + H+
-
r
carnitine + NAD+
-
Agrobacterium sp. 3-dehydrocarnitine + NADH + H+
-
r
carnitine amide + NAD+ L-carnitine dehydrogenase, weak activity Agrobacterium sp. 3-dehydrocarnitine amide + NADH + H+
-
?

Subunits

Subunits Comment Organism
dimer 2 * 57000, L-carnitine dehydrogenase, SDS-PAGE Agrobacterium sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
L-carnitine dehydrogenase Agrobacterium sp.
50
-
-
Alcaligenes sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6 6.5 L-carnitine dehydrogenase, oxidation of L-carnitine Agrobacterium sp.
9
-
oxidation of L-carnitine Alcaligenes sp.
9.5
-
L-carnitine dehydrogenase, oxidation of L-carnitine Agrobacterium sp.

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Pseudomonas putida
NAD+
-
Alcaligenes sp.
NAD+
-
Agrobacterium sp.