BRENDA - Enzyme Database show
show all sequences of 1.1.1.107

Molecular cloning, expression, and properties of an unusual aldo-keto reductase family enzyme, pyridoxal 4-dehydrogenase, that catalyzes irreversible oxidation of pyridoxal

Yokochi, N.; Yoshikane, Y.; Trongpanich, Y.; Ohnishi, K.; Yagi, T.; J. Biol. Chem. 279, 37377-37384 (2004)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
-
Microbacterium luteolum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.042
-
NAD+
cosubstrate L-fucose, pH 8.0, 30C
Microbacterium luteolum
0.05
-
NAD+
cosubstrate pyridoxal, pH 8.0, 30C
Microbacterium luteolum
0.41
-
L-fucose
pH 8.0, 30C
Microbacterium luteolum
0.58
-
NADP+
cosubstrate L-fucose, pH 8.0, 30C
Microbacterium luteolum
0.91
-
D-arabinose
pH 8.0, 30C
Microbacterium luteolum
3.5
-
L-Xylose
pH 8.0, 30C
Microbacterium luteolum
13
-
pyridoxal
pH 8.0, 30C
Microbacterium luteolum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
38000
-
2 * 38000, SDS-PAGE
Microbacterium luteolum
84000
-
gel filtration
Microbacterium luteolum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Microbacterium luteolum
Q76KC2
strain YK-1
-
Microbacterium luteolum YK-1
Q76KC2
strain YK-1
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
635
-
pH 8.0, 30C
Microbacterium luteolum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-arabinose + NAD+
-
656239
Microbacterium luteolum
? + NADH
-
-
-
?
D-arabinose + NAD+
-
656239
Microbacterium luteolum YK-1
? + NADH
-
-
-
?
L-fucose + NAD+
-
656239
Microbacterium luteolum
? + NADH
-
-
-
?
L-fucose + NAD+
-
656239
Microbacterium luteolum YK-1
? + NADH
-
-
-
?
L-xylose + NAD+
-
656239
Microbacterium luteolum
? + NADH
-
-
-
?
L-xylose + NAD+
-
656239
Microbacterium luteolum YK-1
? + NADH
-
-
-
?
pyridoxal + NAD+
-
656239
Microbacterium luteolum
4-pyridoxolactone + NADH + H+
-
-
-
?
pyridoxal + NAD+
-
656239
Microbacterium luteolum YK-1
4-pyridoxolactone + NADH + H+
-
-
-
?
pyridoxal + NADP+
-
656239
Microbacterium luteolum
4-pyridoxolactone + NADPH + H+
-
-
-
-
pyridoxal + NADP+
-
656239
Microbacterium luteolum
4-pyridoxolactone + NADPH + H+
-
-
-
?
pyridoxal + NADP+
-
656239
Microbacterium luteolum YK-1
4-pyridoxolactone + NADPH + H+
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 38000, SDS-PAGE
Microbacterium luteolum
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
-
Microbacterium luteolum
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
40
-
stable up to
Microbacterium luteolum
45
-
10 min, 75% inactivation
Microbacterium luteolum
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
59.2
-
pyridoxal
pH 8.0, 30C
Microbacterium luteolum
125
-
L-fucose
pH 8.0, 30C
Microbacterium luteolum
175
-
D-arabinose
pH 8.0, 30C
Microbacterium luteolum
214
-
L-Xylose
pH 8.0, 30C
Microbacterium luteolum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
substrate pyridoxal
Microbacterium luteolum
9.5
-
substrate L-fucose
Microbacterium luteolum
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Microbacterium luteolum
NADP+
-
Microbacterium luteolum
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Microbacterium luteolum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Microbacterium luteolum
NADP+
-
Microbacterium luteolum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.042
-
NAD+
cosubstrate L-fucose, pH 8.0, 30C
Microbacterium luteolum
0.05
-
NAD+
cosubstrate pyridoxal, pH 8.0, 30C
Microbacterium luteolum
0.41
-
L-fucose
pH 8.0, 30C
Microbacterium luteolum
0.58
-
NADP+
cosubstrate L-fucose, pH 8.0, 30C
Microbacterium luteolum
0.91
-
D-arabinose
pH 8.0, 30C
Microbacterium luteolum
3.5
-
L-Xylose
pH 8.0, 30C
Microbacterium luteolum
13
-
pyridoxal
pH 8.0, 30C
Microbacterium luteolum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
38000
-
2 * 38000, SDS-PAGE
Microbacterium luteolum
84000
-
gel filtration
Microbacterium luteolum
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
635
-
pH 8.0, 30C
Microbacterium luteolum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-arabinose + NAD+
-
656239
Microbacterium luteolum
? + NADH
-
-
-
?
D-arabinose + NAD+
-
656239
Microbacterium luteolum YK-1
? + NADH
-
-
-
?
L-fucose + NAD+
-
656239
Microbacterium luteolum
? + NADH
-
-
-
?
L-fucose + NAD+
-
656239
Microbacterium luteolum YK-1
? + NADH
-
-
-
?
L-xylose + NAD+
-
656239
Microbacterium luteolum
? + NADH
-
-
-
?
L-xylose + NAD+
-
656239
Microbacterium luteolum YK-1
? + NADH
-
-
-
?
pyridoxal + NAD+
-
656239
Microbacterium luteolum
4-pyridoxolactone + NADH + H+
-
-
-
?
pyridoxal + NAD+
-
656239
Microbacterium luteolum YK-1
4-pyridoxolactone + NADH + H+
-
-
-
?
pyridoxal + NADP+
-
656239
Microbacterium luteolum
4-pyridoxolactone + NADPH + H+
-
-
-
-
pyridoxal + NADP+
-
656239
Microbacterium luteolum
4-pyridoxolactone + NADPH + H+
-
-
-
?
pyridoxal + NADP+
-
656239
Microbacterium luteolum YK-1
4-pyridoxolactone + NADPH + H+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 38000, SDS-PAGE
Microbacterium luteolum
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
-
Microbacterium luteolum
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
40
-
stable up to
Microbacterium luteolum
45
-
10 min, 75% inactivation
Microbacterium luteolum
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
59.2
-
pyridoxal
pH 8.0, 30C
Microbacterium luteolum
125
-
L-fucose
pH 8.0, 30C
Microbacterium luteolum
175
-
D-arabinose
pH 8.0, 30C
Microbacterium luteolum
214
-
L-Xylose
pH 8.0, 30C
Microbacterium luteolum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
substrate pyridoxal
Microbacterium luteolum
9.5
-
substrate L-fucose
Microbacterium luteolum
Other publictions for EC 1.1.1.107
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
721873
Chu
The crystal structure of SDR-t ...
Mesorhizobium loti
Biosci. Biotechnol. Biochem.
75
388-390
2011
-
-
1
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
2
-
-
-
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-
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1
-
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-
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1
1
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-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
710764
Chu
Crystallization and preliminar ...
Mesorhizobium loti
Acta Crystallogr. Sect. F
66
718-720
2010
-
-
1
1
-
-
-
-
-
-
1
-
-
4
-
-
1
-
-
-
1
-
1
1
-
-
-
-
-
-
-
1
-
-
-
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-
1
1
1
-
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1
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1
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1
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1
1
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-
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-
-
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-
688568
Nishimura
Determination of individual vi ...
Saccharomyces cerevisiae
J. Nutr. Sci. Vitaminol.
54
18-24
2008
-
1
-
-
-
-
-
-
-
-
-
1
-
1
-
-
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-
-
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2
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1
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2
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1
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1
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1
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1
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-
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-
2
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
699047
Tamura
Synthesis of 4-pyridoxolactone ...
Escherichia coli, Escherichia coli JM109
J. Biosci. Bioeng.
106
460-465
2008
-
-
1
-
-
-
-
-
-
-
-
-
-
30
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
667329
Yokochi
Identification of a new tetram ...
Mesorhizobium loti
Arch. Biochem. Biophys.
452
1-8
2006
-
-
1
-
-
-
-
2
1
-
4
2
-
3
-
-
1
-
-
-
1
-
4
1
1
1
3
2
1
1
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
2
1
-
4
2
-
-
-
1
-
-
1
-
4
1
1
1
3
2
1
1
-
-
-
-
-
-
-
-
654111
Yokochi
Crystallization and preliminar ...
Microbacterium luteolum
Acta Crystallogr. Sect. D
60
2061-2062
2004
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
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-
-
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-
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1
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-
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-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
656239
Yokochi
Molecular cloning, expression, ...
Microbacterium luteolum, Microbacterium luteolum YK-1
J. Biol. Chem.
279
37377-37384
2004
-
-
1
-
-
-
-
7
-
-
2
-
-
5
-
-
-
-
-
-
1
-
11
1
1
-
2
4
2
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
7
-
-
2
-
-
-
-
-
-
-
1
-
11
1
1
-
2
4
2
-
-
-
-
-
-
-
-
-
655029
Trongpanich
Purification and characterizat ...
Microbacterium luteolum
Biosci. Biotechnol. Biochem.
66
543-548
2002
-
-
-
-
-
-
3
3
-
-
2
-
-
4
-
-
1
-
-
-
-
1
4
2
1
-
1
-
2
-
-
2
-
-
-
-
-
-
2
-
-
-
-
3
-
3
-
-
2
-
-
-
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1
-
-
-
1
4
2
1
-
1
-
2
-
-
-
-
-
-
-
-
-
80781
Burg
The bacterial oxidation of vit ...
Pseudomonas sp.
J. Biol. Chem.
244
2585-2589
1969
1
-
-
-
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-
11
2
-
-
-
2
-
1
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1
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4
-
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-
1
1
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1
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1
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1
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11
-
2
-
-
-
2
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1
-
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-
4
-
-
-
-
-
1
1
-
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