BRENDA - Enzyme Database show
show all sequences of 1.1.1.107

Purification and characterization of pyridoxal 4-dehydrogenase from Aureobacterium luteolum

Trongpanich, Y.; Abe, K.; Kaneda, Y.; Morita, T.; Yagi, T.; Biosci. Biotechnol. Biochem. 66, 543-548 (2002)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
8-hydroxyquinoline
1 mM, 78% inhibition
Microbacterium luteolum
o-phenanthroline
1 mM, 15% inhibition
Microbacterium luteolum
pyridoxal
substrate inhibition, 90% inhibition at 2 mM
Microbacterium luteolum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.133
-
NAD+
30C, pH 8.0
Microbacterium luteolum
0.473
-
pyridoxal
30C, pH 8.0
Microbacterium luteolum
1.41
-
NADP+
30C, pH 8.0
Microbacterium luteolum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
38000
-
2 * 38000, SDS-PAGE
Microbacterium luteolum
80000
-
gel filtration
Microbacterium luteolum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Microbacterium luteolum
-
growth on pyridoxine as carbon and nitrogen source
-
Purification (Commentary)
Commentary
Organism
-
Microbacterium luteolum
Storage Stability
Storage Stability
Organism
4C, 1% 2-mercaptoethanol, 24 h, no loss of activity, 2-mercaptoethanol is essential for stability
Microbacterium luteolum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
no substrate: pyridoxal-5-phosphate, pyridoxamine, pyridoxine, 2-nitrobenzaldehyde, 3-nitrobenzaldehyde, 4-nitrobenzaldehyde, o-phthalaldehyde, 5-nitrosalicylaldehyde, 2-carboxybenzaldehyde
655029
Microbacterium luteolum
?
-
-
-
-
pyridine-4-aldehyde + NAD+
0.9% of the activity against pyridoxal
655029
Microbacterium luteolum
4-pyridoxolactone + NADH + H+
-
-
-
?
pyridoxal + NAD+
-
655029
Microbacterium luteolum
4-pyridoxolactone + NADH + H+
-
-
-
?
pyridoxal + NADP+
-
655029
Microbacterium luteolum
4-pyridoxolactone + NADPH + H+
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 38000, SDS-PAGE
Microbacterium luteolum
More
N-terminal amino acid analysis
Microbacterium luteolum
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
-
Microbacterium luteolum
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
50
-
10 min, no residual activity
Microbacterium luteolum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
8.5
sodium phosphate buffer
Microbacterium luteolum
8.5
-
MOPS buffer
Microbacterium luteolum
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Microbacterium luteolum
NADP+
-
Microbacterium luteolum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Microbacterium luteolum
NADP+
-
Microbacterium luteolum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
8-hydroxyquinoline
1 mM, 78% inhibition
Microbacterium luteolum
o-phenanthroline
1 mM, 15% inhibition
Microbacterium luteolum
pyridoxal
substrate inhibition, 90% inhibition at 2 mM
Microbacterium luteolum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.133
-
NAD+
30C, pH 8.0
Microbacterium luteolum
0.473
-
pyridoxal
30C, pH 8.0
Microbacterium luteolum
1.41
-
NADP+
30C, pH 8.0
Microbacterium luteolum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
38000
-
2 * 38000, SDS-PAGE
Microbacterium luteolum
80000
-
gel filtration
Microbacterium luteolum
Purification (Commentary) (protein specific)
Commentary
Organism
-
Microbacterium luteolum
Storage Stability (protein specific)
Storage Stability
Organism
4C, 1% 2-mercaptoethanol, 24 h, no loss of activity, 2-mercaptoethanol is essential for stability
Microbacterium luteolum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
no substrate: pyridoxal-5-phosphate, pyridoxamine, pyridoxine, 2-nitrobenzaldehyde, 3-nitrobenzaldehyde, 4-nitrobenzaldehyde, o-phthalaldehyde, 5-nitrosalicylaldehyde, 2-carboxybenzaldehyde
655029
Microbacterium luteolum
?
-
-
-
-
pyridine-4-aldehyde + NAD+
0.9% of the activity against pyridoxal
655029
Microbacterium luteolum
4-pyridoxolactone + NADH + H+
-
-
-
?
pyridoxal + NAD+
-
655029
Microbacterium luteolum
4-pyridoxolactone + NADH + H+
-
-
-
?
pyridoxal + NADP+
-
655029
Microbacterium luteolum
4-pyridoxolactone + NADPH + H+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 38000, SDS-PAGE
Microbacterium luteolum
More
N-terminal amino acid analysis
Microbacterium luteolum
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
-
Microbacterium luteolum
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
50
-
10 min, no residual activity
Microbacterium luteolum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
8.5
sodium phosphate buffer
Microbacterium luteolum
8.5
-
MOPS buffer
Microbacterium luteolum
Other publictions for EC 1.1.1.107
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
721873
Chu
The crystal structure of SDR-t ...
Mesorhizobium loti
Biosci. Biotechnol. Biochem.
75
388-390
2011
-
-
1
-
-
-
-
-
-
-
-
1
-
2
-
-
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-
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2
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1
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1
1
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1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
3
3
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-
-
710764
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Crystallization and preliminar ...
Mesorhizobium loti
Acta Crystallogr. Sect. F
66
718-720
2010
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-
1
1
-
-
-
-
-
-
1
-
-
4
-
-
1
-
-
-
1
-
1
1
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-
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-
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1
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1
1
1
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1
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1
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1
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1
1
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-
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-
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-
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688568
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Determination of individual vi ...
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J. Nutr. Sci. Vitaminol.
54
18-24
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1
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-
-
-
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1
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1
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-
-
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-
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-
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2
-
1
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2
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1
-
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1
-
1
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-
-
-
-
-
-
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-
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
699047
Tamura
Synthesis of 4-pyridoxolactone ...
Escherichia coli, Escherichia coli JM109
J. Biosci. Bioeng.
106
460-465
2008
-
-
1
-
-
-
-
-
-
-
-
-
-
30
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
667329
Yokochi
Identification of a new tetram ...
Mesorhizobium loti
Arch. Biochem. Biophys.
452
1-8
2006
-
-
1
-
-
-
-
2
1
-
4
2
-
3
-
-
1
-
-
-
1
-
4
1
1
1
3
2
1
1
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
2
1
-
4
2
-
-
-
1
-
-
1
-
4
1
1
1
3
2
1
1
-
-
-
-
-
-
-
-
654111
Yokochi
Crystallization and preliminar ...
Microbacterium luteolum
Acta Crystallogr. Sect. D
60
2061-2062
2004
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
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1
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-
-
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-
-
-
-
656239
Yokochi
Molecular cloning, expression, ...
Microbacterium luteolum, Microbacterium luteolum YK-1
J. Biol. Chem.
279
37377-37384
2004
-
-
1
-
-
-
-
7
-
-
2
-
-
5
-
-
-
-
-
-
1
-
11
1
1
-
2
4
2
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
7
-
-
2
-
-
-
-
-
-
-
1
-
11
1
1
-
2
4
2
-
-
-
-
-
-
-
-
-
655029
Trongpanich
Purification and characterizat ...
Microbacterium luteolum
Biosci. Biotechnol. Biochem.
66
543-548
2002
-
-
-
-
-
-
3
3
-
-
2
-
-
4
-
-
1
-
-
-
-
1
4
2
1
-
1
-
2
-
-
2
-
-
-
-
-
-
2
-
-
-
-
3
-
3
-
-
2
-
-
-
-
1
-
-
-
1
4
2
1
-
1
-
2
-
-
-
-
-
-
-
-
-
80781
Burg
The bacterial oxidation of vit ...
Pseudomonas sp.
J. Biol. Chem.
244
2585-2589
1969
1
-
-
-
-
-
11
2
-
-
-
2
-
1
-
-
1
-
-
-
-
-
4
-
-
-
-
-
1
1
-
1
-
-
-
1
-
-
1
-
-
-
-
11
-
2
-
-
-
2
-
-
-
1
-
-
-
-
4
-
-
-
-
-
1
1
-
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