BRENDA - Enzyme Database show
show all sequences of 1.1.1.106

The bacterial degradation of pantothenic acid. II. Enzymatic formation of aldopantoic acid

Goodhue, C.T.; Snell, E.E.; Biochemistry 5, 403-408 (1966)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.033
-
D-pantoate
at pH 10.0
Pseudomonas sp. P2
0.5
-
ketopantoate
-
Pseudomonas sp. P2
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(R)-pantoate + NAD+
Pseudomonas sp. P2
-
(R)-4-dehydropantoate + NADH
-
Pseudomonas sp. P2
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas sp. P2
-
-
-
Purification (Commentary)
Commentary
Organism
50°C, protamine sulfate, calcium phosphate gel, ammonium sulfate, 50°C
Pseudomonas sp. P2
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6
-
-
Pseudomonas sp. P2
Storage Stability
Storage Stability
Organism
-20°C, 2 mM potassium phosphate, pH 7.0, 0.01% EDTA, 1 mM mercaptoethanol, several months, no loss of activity
Pseudomonas sp. P2
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R)-pantoate + NAD+
-
285731
Pseudomonas sp. P2
(R)-4-dehydropantoate + NADH
-
285731
Pseudomonas sp. P2
r
2-oxo-pantoate + NAD+
-
285731
Pseudomonas sp. P2
2-oxo-4-dehydropantoate
-
285731
Pseudomonas sp. P2
?
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7
10
linear activity increase
Pseudomonas sp. P2
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Pseudomonas sp. P2
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Pseudomonas sp. P2
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.033
-
D-pantoate
at pH 10.0
Pseudomonas sp. P2
0.5
-
ketopantoate
-
Pseudomonas sp. P2
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(R)-pantoate + NAD+
Pseudomonas sp. P2
-
(R)-4-dehydropantoate + NADH
-
Pseudomonas sp. P2
r
Purification (Commentary) (protein specific)
Commentary
Organism
50°C, protamine sulfate, calcium phosphate gel, ammonium sulfate, 50°C
Pseudomonas sp. P2
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6
-
-
Pseudomonas sp. P2
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, 2 mM potassium phosphate, pH 7.0, 0.01% EDTA, 1 mM mercaptoethanol, several months, no loss of activity
Pseudomonas sp. P2
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R)-pantoate + NAD+
-
285731
Pseudomonas sp. P2
(R)-4-dehydropantoate + NADH
-
285731
Pseudomonas sp. P2
r
2-oxo-pantoate + NAD+
-
285731
Pseudomonas sp. P2
2-oxo-4-dehydropantoate
-
285731
Pseudomonas sp. P2
?
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7
10
linear activity increase
Pseudomonas sp. P2
Other publictions for EC 1.1.1.106
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
285729
Myöhänen
Evidence for the importance of ...
Pseudomonas fluorescens, Pseudomonas fluorescens UK1
Biochim. Biophys. Acta
614
266-273
1980
-
-
-
-
-
1
4
1
-
-
-
2
-
4
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
1
-
4
-
1
-
-
-
2
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
207979
Mäntsälä
Purification of pantoate and d ...
Pseudomonas fluorescens, Pseudomonas fluorescens UK1
Biochim. Biophys. Acta
526
25-33
1978
-
-
-
-
-
-
-
-
-
-
3
2
-
4
-
-
1
-
-
-
1
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
3
2
-
-
-
1
-
-
1
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285731
Goodhue
The bacterial degradation of p ...
Pseudomonas sp. P2
Biochemistry
5
403-408
1966
-
-
-
-
-
-
-
2
-
-
-
1
-
1
-
-
1
-
-
-
1
1
2
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
-
-
1
-
-
-
1
-
-
1
1
2
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-