BRENDA - Enzyme Database show
show all sequences of 1.1.1.106

Purification of pantoate and dimethylmalate dehydrogenase from Pseudomonas fluorescens UK-1

Mäntsälä, P.; Biochim. Biophys. Acta 526, 25-33 (1978)

Data extracted from this reference:

Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
24000
-
4 * 24000, SDS-PAGE
Pseudomonas fluorescens
80000
-
ultracentrifugation
Pseudomonas fluorescens
86000
-
gel filtration
Pseudomonas fluorescens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(R)-pantoate + NAD+
Pseudomonas fluorescens
-
(R)-4-dehydropantoate + NADH
-
Pseudomonas fluorescens
-
(R)-pantoate + NAD+
Pseudomonas fluorescens UK1
-
(R)-4-dehydropantoate + NADH
-
Pseudomonas fluorescens UK1
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas fluorescens
-
UK1
-
Pseudomonas fluorescens UK1
-
UK1
-
Purification (Commentary)
Commentary
Organism
ammonium sulfate, 50°C, gel filtration, hydroxyapatite, 60°C, gel filtration
Pseudomonas fluorescens
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
19.34
-
-
Pseudomonas fluorescens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R)-pantoate + NAD+
-
207979
Pseudomonas fluorescens
(R)-4-dehydropantoate + NADH
-
207979
Pseudomonas fluorescens
-
(R)-pantoate + NAD+
-
207979
Pseudomonas fluorescens UK1
(R)-4-dehydropantoate + NADH
-
207979
Pseudomonas fluorescens UK1
-
Subunits
Subunits
Commentary
Organism
tetramer
4 * 24000, SDS-PAGE
Pseudomonas fluorescens
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Pseudomonas fluorescens
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Pseudomonas fluorescens
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
24000
-
4 * 24000, SDS-PAGE
Pseudomonas fluorescens
80000
-
ultracentrifugation
Pseudomonas fluorescens
86000
-
gel filtration
Pseudomonas fluorescens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(R)-pantoate + NAD+
Pseudomonas fluorescens
-
(R)-4-dehydropantoate + NADH
-
Pseudomonas fluorescens
-
(R)-pantoate + NAD+
Pseudomonas fluorescens UK1
-
(R)-4-dehydropantoate + NADH
-
Pseudomonas fluorescens UK1
-
Purification (Commentary) (protein specific)
Commentary
Organism
ammonium sulfate, 50°C, gel filtration, hydroxyapatite, 60°C, gel filtration
Pseudomonas fluorescens
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
19.34
-
-
Pseudomonas fluorescens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R)-pantoate + NAD+
-
207979
Pseudomonas fluorescens
(R)-4-dehydropantoate + NADH
-
207979
Pseudomonas fluorescens
-
(R)-pantoate + NAD+
-
207979
Pseudomonas fluorescens UK1
(R)-4-dehydropantoate + NADH
-
207979
Pseudomonas fluorescens UK1
-
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 24000, SDS-PAGE
Pseudomonas fluorescens
Other publictions for EC 1.1.1.106
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
285729
Myöhänen
Evidence for the importance of ...
Pseudomonas fluorescens, Pseudomonas fluorescens UK1
Biochim. Biophys. Acta
614
266-273
1980
-
-
-
-
-
1
4
1
-
-
-
2
-
4
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
1
-
4
-
1
-
-
-
2
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
207979
Mäntsälä
Purification of pantoate and d ...
Pseudomonas fluorescens, Pseudomonas fluorescens UK1
Biochim. Biophys. Acta
526
25-33
1978
-
-
-
-
-
-
-
-
-
-
3
2
-
4
-
-
1
-
-
-
1
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
3
2
-
-
-
1
-
-
1
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285731
Goodhue
The bacterial degradation of p ...
Pseudomonas sp. P2
Biochemistry
5
403-408
1966
-
-
-
-
-
-
-
2
-
-
-
1
-
1
-
-
1
-
-
-
1
1
2
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
-
-
1
-
-
-
1
-
-
1
1
2
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-