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Literature summary for 1.1.1.105 extracted from

  • Hong, S.H.; Ngo, H.P.; Kang, L.W.; Oh, D.K.
    Characterization of alcohol dehydrogenase from Kangiella koreensis and its application to production of all-trans-retinol (2015), Biotechnol. Lett., 37, 849-856.
    View publication on PubMed

Application

Application Comment Organism
synthesis under optimized conditions, the enzyme produces 600 mg all-trans-retinol per l after 3 h, with a conversion yield of 27.3% (w/w) and a productivity of 200 mg per l and h Kangiella koreensis

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli ER2566 cells Kangiella koreensis
expression in Escherichia coli Kangiella koreensis

Inhibitors

Inhibitors Comment Organism Structure
additional information the enzyme is not influenced by EDTA Kangiella koreensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0018
-
all-trans-retinal at pH 6.5 and 60°C Kangiella koreensis

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the enzyme activity is not affected by monovalent or divalent cations Kangiella koreensis
Zn2+ contains Zn2+ Kangiella koreensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39900
-
-
Kangiella koreensis
40000
-
-
Kangiella koreensis
40000
-
2 * 40000, SDS-PAGE Kangiella koreensis
80000
-
gel filtration Kangiella koreensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
all-trans-retinal + NADH + H+ Kangiella koreensis
-
all-trans-retinol + NAD+
-
r
all-trans-retinal + NADH + H+ Kangiella koreensis SW-125
-
all-trans-retinol + NAD+
-
r
all-trans-retinol + NAD+ Kangiella koreensis
-
all-trans-retinal + NADH + H+
-
r
all-trans-retinol + NAD+ Kangiella koreensis SW-125
-
all-trans-retinal + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Kangiella koreensis
-
-
-
Kangiella koreensis C7R702
-
-
Kangiella koreensis SW-125
-
-
-

Purification (Commentary)

Purification (Comment) Organism
immobilized metal ion affinity chromatography Kangiella koreensis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0213
-
pH 6.5, 60°C Kangiella koreensis
0.0213
-
with all-trans-retinal as substrate, at pH 6.5 and 60°C Kangiella koreensis
0.024
-
with all-trans-retinol as substrate, at pH 6.5 and 60°C Kangiella koreensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
all-trans retinal + NADH + H+ 26.8% of the activity with butan-1-ol Kangiella koreensis all-trans retinol + NAD+
-
?
all-trans-retinal + NADH + H+
-
Kangiella koreensis all-trans-retinol + NAD+
-
r
all-trans-retinal + NADH + H+ 26% activity compared to butyl alcohol Kangiella koreensis all-trans-retinol + NAD+
-
r
all-trans-retinal + NADH + H+
-
Kangiella koreensis SW-125 all-trans-retinol + NAD+
-
r
all-trans-retinal + NADH + H+ 26% activity compared to butyl alcohol Kangiella koreensis SW-125 all-trans-retinol + NAD+
-
r
all-trans-retinol + NAD+
-
Kangiella koreensis all-trans-retinal + NADH + H+
-
r
all-trans-retinol + NAD+ 30.2% activity compared to butyl alcohol Kangiella koreensis all-trans-retinal + NADH + H+
-
?
all-trans-retinol + NAD+
-
Kangiella koreensis SW-125 all-trans-retinal + NADH + H+
-
r
all-trans-retinol + NAD+ 30.2% activity compared to butyl alcohol Kangiella koreensis SW-125 all-trans-retinal + NADH + H+
-
?
butyl alcohol + NAD+ 100% activity Kangiella koreensis butanal + NADH + H+
-
?
butyl alcohol + NAD+ 100% activity Kangiella koreensis SW-125 butanal + NADH + H+
-
?
additional information enzyme is an alcohol dehydrogenase, EC 1.1.1.1, with activity for all-trans-retinol using NAD+ as a cofactor Kangiella koreensis ?
-
?

Subunits

Subunits Comment Organism
? x * 40000, SDS-PAGE, x * 39900, calculated Kangiella koreensis
homodimer 2 * 40000, SDS-PAGE Kangiella koreensis
homodimer 2 * 39900, cslculated from amino acid sequence Kangiella koreensis

Synonyms

Synonyms Comment Organism
ADH
-
Kangiella koreensis
alcohol dehydrogenase
-
Kangiella koreensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Kangiella koreensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.028
-
all-trans-retinal at pH 6.5 and 60°C Kangiella koreensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Kangiella koreensis

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Kangiella koreensis
NADH
-
Kangiella koreensis
NADP+
-
Kangiella koreensis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
15.7
-
all-trans-retinal at pH 6.5 and 60°C Kangiella koreensis