Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
photoreceptor outer segment | - |
Mus musculus | 1750 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
all-trans-retinol-[cellular-retinol-binding-protein] + NAD+ | Mus musculus | - |
all-trans-retinal-[cellular-retinol-binding-protein] + NADH + H+ | - |
? | |
all-trans-retinol-[cellular-retinol-binding-protein] + NAD+ | Mus musculus C57BL/6 x 129/Sv | - |
all-trans-retinal-[cellular-retinol-binding-protein] + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Mus musculus C57BL/6 x 129/Sv | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
eye | RDH8 localizes to photoreceptor outer segments | Mus musculus | - |
retina | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
all-trans-retinol-[cellular-retinol-binding-protein] + NAD+ | - |
Mus musculus | all-trans-retinal-[cellular-retinol-binding-protein] + NADH + H+ | - |
? | |
all-trans-retinol-[cellular-retinol-binding-protein] + NAD+ | - |
Mus musculus C57BL/6 x 129/Sv | all-trans-retinal-[cellular-retinol-binding-protein] + NADH + H+ | - |
? | |
additional information | no activity with decanal | Mus musculus | ? | - |
? | |
additional information | no activity with decanal | Mus musculus C57BL/6 x 129/Sv | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RDH8 | - |
Mus musculus |
retinoid dehydrogenase/reductase | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Mus musculus |
General Information | Comment | Organism |
---|---|---|
evolution | RDHs that catalyze the interconversion of retinal and retinol involved in rhodopsin turnover are members of the family of short chain dehydrogenase/reductases | Mus musculus |
malfunction | outer segments of rods deficient in Rdh8 fail to reduce all-trans-retinal. Following exposure to light, a leak of retinoids from outer to inner segments is detected in rods from both wild-type and knock-out mice. In cells lacking Rdh8 or Rdh12, EC 1.1.1.300, this leak is mainly all-trans-retinal, overview. Retinal reductase activity is lost in RDH12-deficient mutants | Mus musculus |
physiological function | in vertebrate rod cells, retinoid dehydrogenases/reductases are critical for reducing the reactive aldehyde all-trans-retinal that is released by photoactivated rhodopsin, to all-trans-retinol. Reduction of all-trans-retinal in vertebrate rod photoreceptors requires the combined action of RDH8 and RDH12. RDH8 in the outer segment provides most of the activity needed to reduce all-trans-retinal generated by the light response, overview | Mus musculus |