BRENDA - Enzyme Database show
show all sequences of 1.1.1.105

Chen, W.; Smith, S.M.; Napoli, J.L.: Molecular characterization of a mouse short chain dehydrogenase/reductase active with all-trans-retinol in intact cells, mRDH1

Zhang, M.; J. Biol. Chem. 276, 44083-44090 (2001)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in CHO-K1 cells
Mus musculus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0022
-
9-cis-retinol
pH 8, 37°C
Mus musculus
0.0026
-
all-trans-retinol
pH 8, 37°C
Mus musculus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
all-trans-retinol + NAD+
Mus musculus
RDH1 functions in a path of all-trans-retinoic acid biosynthesis starting early during embryogenesis
all-trans-retinal + NADH + H+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Mus musculus
Q8VIJ7
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
brain
strong mRNA expression
Mus musculus
-
embryo
the mouse embryo expresses RDH1 as early as 7.0 days post-coitus, and expression is especially intense within the neural tube, gut, and neural crest at embryo day 10.5
Mus musculus
-
epididymis
mRNA expression
Mus musculus
-
eye
mRNA expression
Mus musculus
-
heart
strong mRNA expression
Mus musculus
-
kidney
strong mRNA expression
Mus musculus
-
liver
strong mRNA expression
Mus musculus
-
lung
mRNA expression
Mus musculus
-
additional information
RDH1 has widespread and intense mRNA expression in tissues of embryonic and adult mice
Mus musculus
-
ovary
mRNA expression
Mus musculus
-
pancreas
strong mRNA expression
Mus musculus
-
prostate
mRNA expression
Mus musculus
-
skeletal muscle
strong mRNA expression
Mus musculus
-
smooth muscle
mRNA expression
Mus musculus
-
submandibular gland
strong mRNA expression
Mus musculus
-
testis
strong mRNA expression
Mus musculus
-
thymus
mRNA expression
Mus musculus
-
thyroid gland
mRNA expression
Mus musculus
-
uterus
mRNA expression
Mus musculus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
9-cis-retinol + NAD+
higher activity with all-trans-retinol versus 9-cis-retinol
692959
Mus musculus
9-cis-retinal + NADH + H+
-
-
-
?
all-trans-retinol + NAD+
RDH1 functions in a path of all-trans-retinoic acid biosynthesis starting early during embryogenesis
692959
Mus musculus
all-trans-retinal + NADH + H+
-
-
-
?
all-trans-retinol + NAD+
RDH1 has 8–11fold higher activity with NAD+ versus NADP+. Higher activity with all-trans-retinol versus 9-cis-retinol. Multifunctional enzyme. In addition to retinol dehydrogenase activity, RDH1 has strong 3alpha-hydroxy and weak 17beta-hydroxy steroid dehydrogenase activities
692959
Mus musculus
all-trans-retinal + NADH + H+
-
-
-
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Mus musculus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7.5
8
pH 7.5: 54% of maximal activity, pH 8: maximal activity
Mus musculus
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
RDH1 has 8–11fold higher activity with NAD+ versus NADP+
Mus musculus
NADP+
RDH1 has 8–11fold higher activity with NAD+ versus NADP+
Mus musculus
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in CHO-K1 cells
Mus musculus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
RDH1 has 8–11fold higher activity with NAD+ versus NADP+
Mus musculus
NADP+
RDH1 has 8–11fold higher activity with NAD+ versus NADP+
Mus musculus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0022
-
9-cis-retinol
pH 8, 37°C
Mus musculus
0.0026
-
all-trans-retinol
pH 8, 37°C
Mus musculus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
all-trans-retinol + NAD+
Mus musculus
RDH1 functions in a path of all-trans-retinoic acid biosynthesis starting early during embryogenesis
all-trans-retinal + NADH + H+
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
brain
strong mRNA expression
Mus musculus
-
embryo
the mouse embryo expresses RDH1 as early as 7.0 days post-coitus, and expression is especially intense within the neural tube, gut, and neural crest at embryo day 10.5
Mus musculus
-
epididymis
mRNA expression
Mus musculus
-
eye
mRNA expression
Mus musculus
-
heart
strong mRNA expression
Mus musculus
-
kidney
strong mRNA expression
Mus musculus
-
liver
strong mRNA expression
Mus musculus
-
lung
mRNA expression
Mus musculus
-
additional information
RDH1 has widespread and intense mRNA expression in tissues of embryonic and adult mice
Mus musculus
-
ovary
mRNA expression
Mus musculus
-
pancreas
strong mRNA expression
Mus musculus
-
prostate
mRNA expression
Mus musculus
-
skeletal muscle
strong mRNA expression
Mus musculus
-
smooth muscle
mRNA expression
Mus musculus
-
submandibular gland
strong mRNA expression
Mus musculus
-
testis
strong mRNA expression
Mus musculus
-
thymus
mRNA expression
Mus musculus
-
thyroid gland
mRNA expression
Mus musculus
-
uterus
mRNA expression
Mus musculus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
9-cis-retinol + NAD+
higher activity with all-trans-retinol versus 9-cis-retinol
692959
Mus musculus
9-cis-retinal + NADH + H+
-
-
-
?
all-trans-retinol + NAD+
RDH1 functions in a path of all-trans-retinoic acid biosynthesis starting early during embryogenesis
692959
Mus musculus
all-trans-retinal + NADH + H+
-
-
-
?
all-trans-retinol + NAD+
RDH1 has 8–11fold higher activity with NAD+ versus NADP+. Higher activity with all-trans-retinol versus 9-cis-retinol. Multifunctional enzyme. In addition to retinol dehydrogenase activity, RDH1 has strong 3alpha-hydroxy and weak 17beta-hydroxy steroid dehydrogenase activities
692959
Mus musculus
all-trans-retinal + NADH + H+
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Mus musculus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7.5
8
pH 7.5: 54% of maximal activity, pH 8: maximal activity
Mus musculus
Other publictions for EC 1.1.1.105
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738036
Adams
Characterization of human shor ...
Homo sapiens
Chem. Biol. Interact.
FEHLT
0000
2016
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1
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37
849-856
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1
1
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1
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1
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1
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1
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738033
Belyaeva
Evolutionary origins of retino ...
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Chem. Biol. Interact.
234
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5
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5
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738670
Obrochta
Insulin regulates retinol dehy ...
Homo sapiens, Mus musculus
J. Biol. Chem.
290
7259-7268
2015
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7
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-
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-
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-
3
2
3
5
-
-
738610
Adams
The retinaldehyde reductase ac ...
Homo sapiens
J. Biol. Chem.
289
14868-14880
2014
-
-
1
-
-
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3
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2
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2
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1
1
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-
721724
Napoli
Physiological insights into al ...
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Biochim. Biophys. Acta
1821
152-167
2012
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2
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1
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1
1
6
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3
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3
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1
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6
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3
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2
3
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1
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1
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1
6
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3
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1
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6
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7
7
-
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722769
Chen
Reduction of all-trans-retinal ...
Mus musculus 129/Sv and C57BL/6, Mus musculus
J. Biol. Chem.
287
24662-24670
2012
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2
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4
-
1
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1
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3
3
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723076
Yu
Investigation of the associati ...
Homo sapiens
J. Zhejiang Univ. Sci. B
11
836-841
2010
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1
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1
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1
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690842
Takahashi
Characterization of key residu ...
Homo sapiens
Biochem. J.
419
113-122
2009
-
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1
-
12
-
-
6
1
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1
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1
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1
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2
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1
2
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12
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6
1
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1
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1
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691750
Lee
Biochemical characterization o ...
Homo sapiens
Chem. Biol. Interact.
178
182-187
2009
-
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1
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2
1
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2
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2
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2
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1
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2
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8
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3
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1
3
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2
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1
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2
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2
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1
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2
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8
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697544
Strate
Retinol dehydrogenase 10 is a ...
Xenopus laevis
Development
136
461-472
2009
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1
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1
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1
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2
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1
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1
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1
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2
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691705
Pares
Medium- and short-chain dehydr ...
Homo sapiens, Mus musculus, Rattus norvegicus
Cell. Mol. Life Sci.
65
3936-3949
2008
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3
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2
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697796
Moradi
Focus on molecules: Retinol de ...
Homo sapiens
Exp. Eye Res.
87
160-161
2008
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1
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1
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7
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7
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1
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686662
Dalfo
Analysis of the NADH-dependent ...
Branchiostoma floridae
FEBS J.
274
3739-3752
2007
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1
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4
1
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689771
Maeda
Redundant and unique roles of ...
Mus musculus
Proc. Natl. Acad. Sci. USA
104
19565-19570
2007
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667537
Gallego
Comparative functional analysi ...
Homo sapiens
Biochem. J.
399
101-109
2006
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1
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1
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3
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1
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669445
Liden
Understanding retinol metaboli ...
Bos taurus, Homo sapiens, Mus musculus
J. Biol. Chem.
281
13001-13004
2006
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3
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6
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6
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656260
Zhang
Elements in the N-terminal sig ...
Mus musculus
J. Biol. Chem.
279
51482-51489
2004
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1
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654431
Karlsson
13-cis-retinoic acid competiti ...
Homo sapiens
Biochem. Biophys. Res. Commun.
303
273-278
2003
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3
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1
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3
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4
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1
6
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1
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