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Literature summary for 1.1.1.105 extracted from

  • Lee, S.A.; Belyaeva, O.V.; Kedishvili, N.Y.
    Biochemical characterization of human epidermal retinol dehydrogenase 2 (2009), Chem. Biol. Interact., 178, 182-187.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Sf9 insect cells and in HEK-293 cells Homo sapiens
RDH-E2 is expressed in Sf9 insect cells as a fusion to the C-terminal His6-tag Homo sapiens

General Stability

General Stability Organism
gel-filtration of RDH-E2 preparation to remove imidazole results in significant loss of activity (about 90%) despite nearly complete recovery of the protein suggesting that RDH-E2 is sensitive to desalting Homo sapiens
the enzyme is sensitive to desalting Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
imidazole increasing the amount of imidazole in the reaction mixture with purified RDH-E2 from 25 to 225mM significantly decreases RDH-E2 activity Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
Homo sapiens 5783
-
endoplasmic reticulum RDH-E2 protein exhibits a punctuate localization pattern, surrounding the nucleus suggesting that RDH-E2 is associated with endoplasmic reticulum Homo sapiens 5783
-
membrane of endoplasmic reticulum Homo sapiens 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
all-trans-retinol + NAD+ Homo sapiens exhibits a strong preference for NAD+/NADH as cofactors. Activity toward all-trans-retinal in the presence of NADH is 2fold lower than activity with all-trans-retinol and NAD+. The preference for NAD+ suggests that RDH-E2 is likely to function in the oxidative direction in vivo, further supporting its potential role in the oxidation of retinol to retinaldehyde for retinoic acid biosynthesis in human keratinocytes all-trans-retinal + NADH + H+
-
r
additional information Homo sapiens RDH-E2 may be involved in the pathogenesis of psoriasis through its potential role in retinoic acid biosynthesis and stimulation of keratinocyte proliferation ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q8N3Y7
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-NTA resin column chromatography Homo sapiens
recombinant enzyme Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
epidermis retinal dehydrogenase 2 is significantly elevated in psoriatic skin Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
all-trans-retinal + NADH + H+
-
Homo sapiens all-trans-retinol + NAD+
-
?
all-trans-retinaldehyde + NADH + H+
-
Homo sapiens ?
-
?
all-trans-retinol + NAD+ exhibits a strong preference for NAD+/NADH as cofactors. Activity toward all-trans-retinal in the presence of NADH is 2fold lower than activity with all-trans-retinol and NAD+. The preference for NAD+ suggests that RDH-E2 is likely to function in the oxidative direction in vivo, further supporting its potential role in the oxidation of retinol to retinaldehyde for retinoic acid biosynthesis in human keratinocytes Homo sapiens all-trans-retinal + NADH + H+
-
r
all-trans-retinol + NAD+ exhibits a strong preference for NAD+/NADH as cofactors. Activity with NAD+ is about 10fold higher than activity in presence of NADP+. Activity toward all-trans-retinal in the presence of NADH is 2fold lower than activity with all-trans-retinol and NAD+ Homo sapiens all-trans-retinal + NADH + H+
-
r
all-trans-retinol + NADP+ exhibits a strong preference for NAD+/NADH as cofactors. Activity with NAD+ is about 10fold higher than activity in presence of NADP+ Homo sapiens all-trans-retinal + NADPH + H+
-
r
additional information no activity was detected with 11-cis-retinol or 11-cis-retinaldehyde as substrates with either NAD+/NADH or NADP+/NADPH Homo sapiens ?
-
?
additional information RDH-E2 may be involved in the pathogenesis of psoriasis through its potential role in retinoic acid biosynthesis and stimulation of keratinocyte proliferation Homo sapiens ?
-
?
additional information no activity is detected with 11-cis-retinol or 11-cis-retinaldehyde as substrates with either NAD+/NADH or NADP+/NADPH Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
epidermal retinol dehydrogenase 2
-
Homo sapiens
RDH-E2
-
Homo sapiens
retinol dehydrogenase 2
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD+ exhibits a strong preference for NAD+/NADH as cofactors. Activity with NAD+ is about 10fold higher than activity in presence of NADP+ Homo sapiens
NADH exhibits a strong preference for NAD+/NADH as cofactors Homo sapiens
NADP+ exhibits a strong preference for NAD+/NADH as cofactors. Activity with NAD+ is about 10fold higher than activity in presence of NADP+ Homo sapiens