Cloned (Comment) | Organism |
---|---|
expressed in COS-1 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
G43A/G47A/G49A | the triple mutation completely abolishes the enzymatic activity of RDH10 without affecting its protein level | Homo sapiens |
K214A | the mutation completely abolishes the enzymatic activity of RDH10 without affecting its protein level | Homo sapiens |
K214R | the mutation completely abolishes the enzymatic activity of RDH10 without affecting its protein level | Homo sapiens |
N169A | the mutation completely abolishes the enzymatic activity of RDH10 without affecting its protein level | Homo sapiens |
N169D | the mutation completely abolishes the enzymatic activity of RDH10 without affecting its protein level | Homo sapiens |
S197A | mutant retains significant enzymatic activities, although lower than that of wild type enzyme | Homo sapiens |
S197C | the mutation completely abolishes the enzymatic activity of RDH10 without affecting its protein level | Homo sapiens |
S197G | mutant retains significant enzymatic activities, although lower than that of wild type enzyme | Homo sapiens |
S197T | the mutation completely abolishes the enzymatic activity of RDH10 without affecting its protein level | Homo sapiens |
S197V | the mutation completely abolishes the enzymatic activity of RDH10 without affecting its protein level | Homo sapiens |
Y210A | the mutation completely abolishes the enzymatic activity of RDH10 without affecting its protein level | Homo sapiens |
Y210F | the mutation completely abolishes the enzymatic activity of RDH10 without affecting its protein level | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0015 | - |
NADPH | wild type enzyme, in 100 mM sodium phosphate, pH 7.4 | Homo sapiens | |
0.0041 | - |
all-trans-retinol | wild type enzyme, in 100 mM sodium phosphate, pH 7.4 | Homo sapiens | |
0.027 | - |
NADP+ | wild type enzyme, in 100 mM sodium phosphate, pH 7.4 | Homo sapiens | |
0.036 | - |
NAD+ | wild type enzyme, in 100 mM sodium phosphate, pH 7.4 | Homo sapiens | |
0.11 | - |
NADH | wild type enzyme, in 100 mM sodium phosphate, pH 7.4 | Homo sapiens | |
0.57 | - |
all-trans-retinal | wild type enzyme, in 100 mM sodium phosphate, pH 7.4 | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Homo sapiens | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q8IZV5 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
all-trans-retinal + NADH + H+ | wild type RDH10 catalyzes both oxidation of all-trans retinol and reduction of all-trans retinal in vitro. On cultured cells, however, oxidation is the favored reaction catalyzed by RDH10. | Homo sapiens | all-trans-retinol + NAD+ | - |
? | |
all-trans-retinal-[cellular-retinol-binding-protein] + NADPH + H+ | - |
Homo sapiens | all-trans-retinol-[cellular-retinol-binding-protein] + NADP+ | - |
? | |
all-trans-retinol-[cellular-retinol-binding-protein] + NADP+ | - |
Homo sapiens | all-trans-retinal-[cellular-retinol-binding-protein] + NADPH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
RDH10 | - |
Homo sapiens |
retinol dehydrogenase 10 | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | the enzyme has higher affinity for NADH than for NADPH | Homo sapiens | |
NADPH | the enzyme has lower affinity for NADPH than for NADH | Homo sapiens |