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Literature summary for 1.1.1.105 extracted from

  • Dalfo, D.; Marques, N.; Albalat, R.
    Analysis of the NADH-dependent retinaldehyde reductase activity of amphioxus retinol dehydrogenase enzymes enhances our understanding of the evolution of the retinol dehydrogenase family (2007), FEBS J., 274, 3739-3752.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
both isoforms 1 and 2, expression in COS-7 cell with N-terminal His-tag Branchiostoma floridae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0087
-
all-trans-retinal isoform 1, pH 6.0, 37°C Branchiostoma floridae
0.0089
-
all-trans-retinal isoform 2, pH 6.0, 37°C Branchiostoma floridae
0.098
-
NADH isoform 2, pH 6.0, 37°C Branchiostoma floridae
0.224
-
NADH isoform 1, pH 6.0, 37°C Branchiostoma floridae

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum both isoforms 1 and 2, associated with endoplasmic reticulum with no nuclear or plasma membrane localization Branchiostoma floridae 5783
-

Organism

Organism UniProt Comment Textmining
Branchiostoma floridae
-
isoforms 1 and 2
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00025
-
per mg of microsome, isoform 1 Branchiostoma floridae
0.0014
-
per mg of microsome, isoform 2 Branchiostoma floridae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
all-trans retinal + NADH + H+ oxidative activity is below the detection limit Branchiostoma floridae all-trans-retinol + NAD+
-
r
additional information negligible activity with 9-cis-retinol Branchiostoma floridae ?
-
?

Cofactor

Cofactor Comment Organism Structure
additional information no significant activity with NADPH Branchiostoma floridae
NAD+
-
Branchiostoma floridae
NADH
-
Branchiostoma floridae