Cloned (Comment) | Organism |
---|---|
both isoforms 1 and 2, expression in COS-7 cell with N-terminal His-tag | Branchiostoma floridae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0087 | - |
all-trans-retinal | isoform 1, pH 6.0, 37°C | Branchiostoma floridae | |
0.0089 | - |
all-trans-retinal | isoform 2, pH 6.0, 37°C | Branchiostoma floridae | |
0.098 | - |
NADH | isoform 2, pH 6.0, 37°C | Branchiostoma floridae | |
0.224 | - |
NADH | isoform 1, pH 6.0, 37°C | Branchiostoma floridae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | both isoforms 1 and 2, associated with endoplasmic reticulum with no nuclear or plasma membrane localization | Branchiostoma floridae | 5783 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Branchiostoma floridae | - |
isoforms 1 and 2 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00025 | - |
per mg of microsome, isoform 1 | Branchiostoma floridae |
0.0014 | - |
per mg of microsome, isoform 2 | Branchiostoma floridae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
all-trans retinal + NADH + H+ | oxidative activity is below the detection limit | Branchiostoma floridae | all-trans-retinol + NAD+ | - |
r | |
additional information | negligible activity with 9-cis-retinol | Branchiostoma floridae | ? | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no significant activity with NADPH | Branchiostoma floridae | |
NAD+ | - |
Branchiostoma floridae | |
NADH | - |
Branchiostoma floridae |